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Dietary fat source regulates ob gene expression in white adipose tissue of rats under hyperphagic feeding.
Br J Nutr. 2002 May; 87(5):427-34.BJ

Abstract

This work was designed to investigate the effect of different lipid sources on ob gene expression and serum leptin levels in rats with two different feeding protocols: (1) free access to food; or (2) energy-controlled feeding. Male Wistar rats were fed diets containing 40 % energy as fat (olive oil, sunflower oil or beef tallow), for 4 weeks. In Expt 1 rats had free access to food, and in Expt 2 rats were fed a controlled amount of food (16 g/d, equivalent to 300 kJ/d). Insulin and leptin were determined by ELISA and ob mRNA by Northern blot. When rats had free access to food, ob mRNA levels were higher in animals fed either olive oil or sunflower oil than in those fed beef tallow. In marked contrast with feeding ad libitum, no differences were found among dietary fat groups in rats fed energy-controlled diets. When both feeding protocols were compared, free access to food induced an increased expression of ob mRNA in perirenal and/or epididymal adipose tissues from rats fed either olive oil or sunflower oil, but not from rats fed beef tallow. Dietary lipid type did not induce modifications in serum leptin concentrations. A tendency to higher serum leptin levels was observed more in rats with free access to food than in rats fed energy-controlled feeding. No differences were found in insulin levels. Dietary fat type importantly affects ob mRNA expression in rat white adipose tissue under hyperphagic conditions. Further study is needed in order to elucidate the mechanism underlying this effect.

Authors+Show Affiliations

Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12010582

Citation

Rodríguez, Víctor M., et al. "Dietary Fat Source Regulates Ob Gene Expression in White Adipose Tissue of Rats Under Hyperphagic Feeding." The British Journal of Nutrition, vol. 87, no. 5, 2002, pp. 427-34.
Rodríguez VM, Picó C, Portillo MP, et al. Dietary fat source regulates ob gene expression in white adipose tissue of rats under hyperphagic feeding. Br J Nutr. 2002;87(5):427-34.
Rodríguez, V. M., Picó, C., Portillo, M. P., Teresa Macarulla, M., & Palou, A. (2002). Dietary fat source regulates ob gene expression in white adipose tissue of rats under hyperphagic feeding. The British Journal of Nutrition, 87(5), 427-34.
Rodríguez VM, et al. Dietary Fat Source Regulates Ob Gene Expression in White Adipose Tissue of Rats Under Hyperphagic Feeding. Br J Nutr. 2002;87(5):427-34. PubMed PMID: 12010582.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary fat source regulates ob gene expression in white adipose tissue of rats under hyperphagic feeding. AU - Rodríguez,Víctor M, AU - Picó,Catalina, AU - Portillo,María P, AU - Teresa Macarulla,M, AU - Palou,Andreu, PY - 2002/5/16/pubmed PY - 2002/6/20/medline PY - 2002/5/16/entrez SP - 427 EP - 34 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 87 IS - 5 N2 - This work was designed to investigate the effect of different lipid sources on ob gene expression and serum leptin levels in rats with two different feeding protocols: (1) free access to food; or (2) energy-controlled feeding. Male Wistar rats were fed diets containing 40 % energy as fat (olive oil, sunflower oil or beef tallow), for 4 weeks. In Expt 1 rats had free access to food, and in Expt 2 rats were fed a controlled amount of food (16 g/d, equivalent to 300 kJ/d). Insulin and leptin were determined by ELISA and ob mRNA by Northern blot. When rats had free access to food, ob mRNA levels were higher in animals fed either olive oil or sunflower oil than in those fed beef tallow. In marked contrast with feeding ad libitum, no differences were found among dietary fat groups in rats fed energy-controlled diets. When both feeding protocols were compared, free access to food induced an increased expression of ob mRNA in perirenal and/or epididymal adipose tissues from rats fed either olive oil or sunflower oil, but not from rats fed beef tallow. Dietary lipid type did not induce modifications in serum leptin concentrations. A tendency to higher serum leptin levels was observed more in rats with free access to food than in rats fed energy-controlled feeding. No differences were found in insulin levels. Dietary fat type importantly affects ob mRNA expression in rat white adipose tissue under hyperphagic conditions. Further study is needed in order to elucidate the mechanism underlying this effect. SN - 0007-1145 UR - https://www.unboundmedicine.com/medline/citation/12010582/Dietary_fat_source_regulates_ob_gene_expression_in_white_adipose_tissue_of_rats_under_hyperphagic_feeding_ L2 - https://medlineplus.gov/dietaryfats.html DB - PRIME DP - Unbound Medicine ER -