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Neuronal degeneration and glial cell-responses following trimethyltin intoxication in the rat.
Acta Neuropathol 2002; 103(6):575-82AN

Abstract

Trimethyltin (TMT) preferentially induces neuronal changes in the hippocampus and pyriform cortex. In the present study we investigated the time course of microglial and astroglial responses associated with neurodegeneration after the administration of TMT (i.p. 9 mg/kg or 12 mg/kg body weight) in the rat. At a dosage of 9 mg/kg TMT, neurodegeneration was clearly demonstrated in the CA1 and CA3 regions of the hippocampus as argyrophilic (dark) neurons by day 4 using the Gallyas-Braak (G-B) impregnation method that has been shown to be sensitive and specific for neurodegeneration. Early microglial response was immunohistochemically shown with anti-microglial response factor-1 (MRF-1) antibody in the CA3 by day 1, preceding neurodegeneration morphologically detected by the G-B method. Activation of astrocytes was revealed by immunohistochemical staining for glial fibrillary acidic protein (GFAP) by day 2. In parallel with the maximal neurodegeneration, large numbers of hypertrophied microglia and astrocytes were observed in the CA1 and CA3 by day 7. Numbers of degenerative neurons appeared to be closely associated with adjacent microglia by the double staining of G-B impregnation and MRF-1 immunohistochemistry. The number of reactive microglia considerably decreased to the resting state by day 14, while hypertrophied astrocytes were still prominent in the CA3 up to day 21. With the high dose of TMT, granule cells in the dentate gyrus and CA1 and CA3 pyramidal cells were significantly impregnated. After TMT treatment, accompaning neurodegeneration we observed early response of microglia and prolonged activation of astrocytes, suggesting an individual role of glial cells in maintenance and repair of damaged neurons following brain injury.

Authors+Show Affiliations

Department of Schizophrenia Research, Division of Psychiatric Disorders Research, Tokyo Institute of Psychiatry, 2-1-8 Kamikitazawa, Setagaya-ku 156-8585, Japan. shaga@prit.go.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12012089

Citation

Haga, S, et al. "Neuronal Degeneration and Glial Cell-responses Following Trimethyltin Intoxication in the Rat." Acta Neuropathologica, vol. 103, no. 6, 2002, pp. 575-82.
Haga S, Haga C, Aizawa T, et al. Neuronal degeneration and glial cell-responses following trimethyltin intoxication in the rat. Acta Neuropathol. 2002;103(6):575-82.
Haga, S., Haga, C., Aizawa, T., & Ikeda, K. (2002). Neuronal degeneration and glial cell-responses following trimethyltin intoxication in the rat. Acta Neuropathologica, 103(6), pp. 575-82.
Haga S, et al. Neuronal Degeneration and Glial Cell-responses Following Trimethyltin Intoxication in the Rat. Acta Neuropathol. 2002;103(6):575-82. PubMed PMID: 12012089.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuronal degeneration and glial cell-responses following trimethyltin intoxication in the rat. AU - Haga,S, AU - Haga,C, AU - Aizawa,T, AU - Ikeda,K, Y1 - 2002/02/06/ PY - 2001/08/16/received PY - 2001/10/15/revised PY - 2001/11/12/accepted PY - 2002/5/16/pubmed PY - 2002/8/8/medline PY - 2002/5/16/entrez SP - 575 EP - 82 JF - Acta neuropathologica JO - Acta Neuropathol. VL - 103 IS - 6 N2 - Trimethyltin (TMT) preferentially induces neuronal changes in the hippocampus and pyriform cortex. In the present study we investigated the time course of microglial and astroglial responses associated with neurodegeneration after the administration of TMT (i.p. 9 mg/kg or 12 mg/kg body weight) in the rat. At a dosage of 9 mg/kg TMT, neurodegeneration was clearly demonstrated in the CA1 and CA3 regions of the hippocampus as argyrophilic (dark) neurons by day 4 using the Gallyas-Braak (G-B) impregnation method that has been shown to be sensitive and specific for neurodegeneration. Early microglial response was immunohistochemically shown with anti-microglial response factor-1 (MRF-1) antibody in the CA3 by day 1, preceding neurodegeneration morphologically detected by the G-B method. Activation of astrocytes was revealed by immunohistochemical staining for glial fibrillary acidic protein (GFAP) by day 2. In parallel with the maximal neurodegeneration, large numbers of hypertrophied microglia and astrocytes were observed in the CA1 and CA3 by day 7. Numbers of degenerative neurons appeared to be closely associated with adjacent microglia by the double staining of G-B impregnation and MRF-1 immunohistochemistry. The number of reactive microglia considerably decreased to the resting state by day 14, while hypertrophied astrocytes were still prominent in the CA3 up to day 21. With the high dose of TMT, granule cells in the dentate gyrus and CA1 and CA3 pyramidal cells were significantly impregnated. After TMT treatment, accompaning neurodegeneration we observed early response of microglia and prolonged activation of astrocytes, suggesting an individual role of glial cells in maintenance and repair of damaged neurons following brain injury. SN - 0001-6322 UR - https://www.unboundmedicine.com/medline/citation/12012089/Neuronal_degeneration_and_glial_cell_responses_following_trimethyltin_intoxication_in_the_rat_ L2 - https://dx.doi.org/10.1007/s00401-001-0505-5 DB - PRIME DP - Unbound Medicine ER -