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A characteristic protein in nasal discharge differentiating non-allergic chronic rhinosinusitis from allergic rhinitis.
Rhinology. 2002 Mar; 40(1):13-7.R

Abstract

To differentiate non-allergic chronic rhinosinusitis (vasomotor rhinitis) from allergic rhinitis, a characteristic protein in the nasal discharge was studied. The subjects consisted of 10 patients with perennial allergic rhinitis to house dust, 10 patients without perennial rhinitis without antigen (clinically defined as non-allergic chronic rhinosinusitis) and 5 normal volunteers without nasal disease as a control group. The total protein in the nasal discharge was determined by Lowry's method and analysis of the protein components was made by SDS-PAGE. It was found that the nasal discharge obtained from the case with perennial allergic rhinitis contained a high concentration of albumin (25.9 micrograms/ml) and a characteristic protein band with an estimated molecular weight of 26 kilo-Daltons (kD) on a SDS-PAGE, in a concentration of 15.8 micrograms/ml. In contrast, the nasal discharge from non-allergic chronic rhinosonusitis patients contained a lower concentration of albumin (12.9 micrograms/ml) than that of the allergic rhinitis patients, and the concentration of the characteristic protein 26 kD was only 2.3 micrograms/ml. The 26 kD protein was considered to originate from the nasal glands, since its secretion could be provoked by stimulation of the nasal glands of the normal volunteers with a 1% pilocarpine spray. The low concentration of albumin and the characteristic protein 26 kD in the nasal discharge thus appeared to differentiate patients with non-allergic chronic rhinosinusitis from those with perennial allergic rhinitis.

Authors+Show Affiliations

Department of Otorhinolaryngology, Kitasato University, School of Medicine, Kanagawa, Japan. iguchi@med.kitasato-u.ac.jpNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

12012948

Citation

Iguchi, Yoshiaki, et al. "A Characteristic Protein in Nasal Discharge Differentiating Non-allergic Chronic Rhinosinusitis From Allergic Rhinitis." Rhinology, vol. 40, no. 1, 2002, pp. 13-7.
Iguchi Y, Yao K, Okamoto M. A characteristic protein in nasal discharge differentiating non-allergic chronic rhinosinusitis from allergic rhinitis. Rhinology. 2002;40(1):13-7.
Iguchi, Y., Yao, K., & Okamoto, M. (2002). A characteristic protein in nasal discharge differentiating non-allergic chronic rhinosinusitis from allergic rhinitis. Rhinology, 40(1), 13-7.
Iguchi Y, Yao K, Okamoto M. A Characteristic Protein in Nasal Discharge Differentiating Non-allergic Chronic Rhinosinusitis From Allergic Rhinitis. Rhinology. 2002;40(1):13-7. PubMed PMID: 12012948.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A characteristic protein in nasal discharge differentiating non-allergic chronic rhinosinusitis from allergic rhinitis. AU - Iguchi,Yoshiaki, AU - Yao,Kazuo, AU - Okamoto,Makito, PY - 2002/5/16/pubmed PY - 2002/11/26/medline PY - 2002/5/16/entrez SP - 13 EP - 7 JF - Rhinology JO - Rhinology VL - 40 IS - 1 N2 - To differentiate non-allergic chronic rhinosinusitis (vasomotor rhinitis) from allergic rhinitis, a characteristic protein in the nasal discharge was studied. The subjects consisted of 10 patients with perennial allergic rhinitis to house dust, 10 patients without perennial rhinitis without antigen (clinically defined as non-allergic chronic rhinosinusitis) and 5 normal volunteers without nasal disease as a control group. The total protein in the nasal discharge was determined by Lowry's method and analysis of the protein components was made by SDS-PAGE. It was found that the nasal discharge obtained from the case with perennial allergic rhinitis contained a high concentration of albumin (25.9 micrograms/ml) and a characteristic protein band with an estimated molecular weight of 26 kilo-Daltons (kD) on a SDS-PAGE, in a concentration of 15.8 micrograms/ml. In contrast, the nasal discharge from non-allergic chronic rhinosonusitis patients contained a lower concentration of albumin (12.9 micrograms/ml) than that of the allergic rhinitis patients, and the concentration of the characteristic protein 26 kD was only 2.3 micrograms/ml. The 26 kD protein was considered to originate from the nasal glands, since its secretion could be provoked by stimulation of the nasal glands of the normal volunteers with a 1% pilocarpine spray. The low concentration of albumin and the characteristic protein 26 kD in the nasal discharge thus appeared to differentiate patients with non-allergic chronic rhinosinusitis from those with perennial allergic rhinitis. SN - 0300-0729 UR - https://www.unboundmedicine.com/medline/citation/12012948/A_characteristic_protein_in_nasal_discharge_differentiating_non_allergic_chronic_rhinosinusitis_from_allergic_rhinitis_ L2 - https://www.rhinologyjournal.com/Abstract.php?id=114 DB - PRIME DP - Unbound Medicine ER -