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TNBS ileitis evokes hyperexcitability and changes in ionic membrane properties of nociceptive DRG neurons.
Am J Physiol Gastrointest Liver Physiol. 2002 Jun; 282(6):G1045-51.AJ

Abstract

This study examines whether intestinal inflammation leads to changes in the properties of ion channels in dorsal root ganglia (DRG) neurons. Ileitis was induced by injection of trinitrobenzene sulfonic acid (TNBS), and DRG neurons innervating the ileum were labeled using fast blue. Intracellular recording techniques were used to measure electrophysiological properties of acutely dissociated neurons 12-24 h after dissection. Nociceptive neurons were identified by sensitivity to capsaicin, tetrodotoxin resistance, and size (<30 microm). The action potential threshold in neurons from TNBS-treated animals was reduced by >70% compared with controls (P < 0.001), but the resting membrane potential was unchanged. Cell diameter, input resistance (67%), and action potential upstroke velocity (22%) increased in the TNBS group (P < 0.05). The number of action potentials discharged increased in the TNBS group (P < 0.001), whereas application of 4-aminopyridine to control cells mimicked this effect. This study demonstrates that ileitis induces hyperexcitability in nociceptive DRG neurons and changes in the properties of Na(+) and K(+) channels at the soma, which persist after removal from the inflamed environment.

Authors+Show Affiliations

Gastrointestinal Diseases Research Unit, Queen's University, Kingston, Ontario, Canada K7L 5G2.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12016130

Citation

Moore, Beverley A., et al. "TNBS Ileitis Evokes Hyperexcitability and Changes in Ionic Membrane Properties of Nociceptive DRG Neurons." American Journal of Physiology. Gastrointestinal and Liver Physiology, vol. 282, no. 6, 2002, pp. G1045-51.
Moore BA, Stewart TM, Hill C, et al. TNBS ileitis evokes hyperexcitability and changes in ionic membrane properties of nociceptive DRG neurons. Am J Physiol Gastrointest Liver Physiol. 2002;282(6):G1045-51.
Moore, B. A., Stewart, T. M., Hill, C., & Vanner, S. J. (2002). TNBS ileitis evokes hyperexcitability and changes in ionic membrane properties of nociceptive DRG neurons. American Journal of Physiology. Gastrointestinal and Liver Physiology, 282(6), G1045-51.
Moore BA, et al. TNBS Ileitis Evokes Hyperexcitability and Changes in Ionic Membrane Properties of Nociceptive DRG Neurons. Am J Physiol Gastrointest Liver Physiol. 2002;282(6):G1045-51. PubMed PMID: 12016130.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TNBS ileitis evokes hyperexcitability and changes in ionic membrane properties of nociceptive DRG neurons. AU - Moore,Beverley A, AU - Stewart,Timothy M R, AU - Hill,Ceredwyn, AU - Vanner,Stephen J, PY - 2002/5/23/pubmed PY - 2002/6/21/medline PY - 2002/5/23/entrez SP - G1045 EP - 51 JF - American journal of physiology. Gastrointestinal and liver physiology JO - Am J Physiol Gastrointest Liver Physiol VL - 282 IS - 6 N2 - This study examines whether intestinal inflammation leads to changes in the properties of ion channels in dorsal root ganglia (DRG) neurons. Ileitis was induced by injection of trinitrobenzene sulfonic acid (TNBS), and DRG neurons innervating the ileum were labeled using fast blue. Intracellular recording techniques were used to measure electrophysiological properties of acutely dissociated neurons 12-24 h after dissection. Nociceptive neurons were identified by sensitivity to capsaicin, tetrodotoxin resistance, and size (<30 microm). The action potential threshold in neurons from TNBS-treated animals was reduced by >70% compared with controls (P < 0.001), but the resting membrane potential was unchanged. Cell diameter, input resistance (67%), and action potential upstroke velocity (22%) increased in the TNBS group (P < 0.05). The number of action potentials discharged increased in the TNBS group (P < 0.001), whereas application of 4-aminopyridine to control cells mimicked this effect. This study demonstrates that ileitis induces hyperexcitability in nociceptive DRG neurons and changes in the properties of Na(+) and K(+) channels at the soma, which persist after removal from the inflamed environment. SN - 0193-1857 UR - https://www.unboundmedicine.com/medline/citation/12016130/TNBS_ileitis_evokes_hyperexcitability_and_changes_in_ionic_membrane_properties_of_nociceptive_DRG_neurons_ L2 - https://journals.physiology.org/doi/10.1152/ajpgi.00406.2001?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -