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Genetic and molecular control of folate-homocysteine metabolism in mutant mice.
Mamm Genome 2002; 13(5):259-67MG

Abstract

Hyperhomocysteinemia adversely affects fundamental aspects of fetal development, adulthood, and aging, but the role of elevated homocysteine levels in these birth defects and adult diseases remains unclear. Mouse models are valuable for investigating the causes and consequences of hyperhomocysteinemia. We used a phenotype-based approach to identify mouse mutants for studying the relation between single gene mutations, homocysteine levels as a measure of the status of homocysteine metabolism, and gene expression profiles as a way to assess the impact of protein deficiency in mutant mice on steady-state transcription levels of genes in the folate-homocysteine pathways. These mutants were selected based on their propensity to produce phenotypes that are reminiscent of those associated with anomalies in folate-homocysteine metabolism in humans. We report identification of new, single-gene mouse models of homocysteinemia and characterization of their molecular and physiological impact on folate-homocysteine metabolism. Mutations in several genes involved in the hedgehog and WNT signal transduction pathways, as well as a gene involved in lipid metabolism, resulted in elevated homocysteine levels and altered expression profiles of folate-homocysteine metabolism genes. These results begin to unravel the complex relations between elevation of a single amino acid in the blood and the diverse birth defects and adult diseases associated with hyperhomocysteinemia.

Authors+Show Affiliations

Department of Genetics, Case Western Reserve University School of Medicine, University Hospitals of Cleveland, 10900 Euclid Avenue, Cleveland, OH 44106, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12016514

Citation

Ernest, Sheila, et al. "Genetic and Molecular Control of Folate-homocysteine Metabolism in Mutant Mice." Mammalian Genome : Official Journal of the International Mammalian Genome Society, vol. 13, no. 5, 2002, pp. 259-67.
Ernest S, Christensen B, Gilfix BM, et al. Genetic and molecular control of folate-homocysteine metabolism in mutant mice. Mamm Genome. 2002;13(5):259-67.
Ernest, S., Christensen, B., Gilfix, B. M., Mamer, O. A., Hosack, A., Rodier, M., ... Nadeau, J. H. (2002). Genetic and molecular control of folate-homocysteine metabolism in mutant mice. Mammalian Genome : Official Journal of the International Mammalian Genome Society, 13(5), pp. 259-67.
Ernest S, et al. Genetic and Molecular Control of Folate-homocysteine Metabolism in Mutant Mice. Mamm Genome. 2002;13(5):259-67. PubMed PMID: 12016514.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic and molecular control of folate-homocysteine metabolism in mutant mice. AU - Ernest,Sheila, AU - Christensen,Benedicte, AU - Gilfix,Brian M, AU - Mamer,Orval A, AU - Hosack,Angela, AU - Rodier,Mitchell, AU - Colmenares,Clemencia, AU - McGrath,James, AU - Bale,Allen, AU - Balling,Rudi, AU - Sankoff,David, AU - Rosenblatt,David S, AU - Nadeau,Joseph H, PY - 2001/06/20/received PY - 2002/02/01/accepted PY - 2002/5/23/pubmed PY - 2002/9/11/medline PY - 2002/5/23/entrez SP - 259 EP - 67 JF - Mammalian genome : official journal of the International Mammalian Genome Society JO - Mamm. Genome VL - 13 IS - 5 N2 - Hyperhomocysteinemia adversely affects fundamental aspects of fetal development, adulthood, and aging, but the role of elevated homocysteine levels in these birth defects and adult diseases remains unclear. Mouse models are valuable for investigating the causes and consequences of hyperhomocysteinemia. We used a phenotype-based approach to identify mouse mutants for studying the relation between single gene mutations, homocysteine levels as a measure of the status of homocysteine metabolism, and gene expression profiles as a way to assess the impact of protein deficiency in mutant mice on steady-state transcription levels of genes in the folate-homocysteine pathways. These mutants were selected based on their propensity to produce phenotypes that are reminiscent of those associated with anomalies in folate-homocysteine metabolism in humans. We report identification of new, single-gene mouse models of homocysteinemia and characterization of their molecular and physiological impact on folate-homocysteine metabolism. Mutations in several genes involved in the hedgehog and WNT signal transduction pathways, as well as a gene involved in lipid metabolism, resulted in elevated homocysteine levels and altered expression profiles of folate-homocysteine metabolism genes. These results begin to unravel the complex relations between elevation of a single amino acid in the blood and the diverse birth defects and adult diseases associated with hyperhomocysteinemia. SN - 0938-8990 UR - https://www.unboundmedicine.com/medline/citation/12016514/Genetic_and_molecular_control_of_folate_homocysteine_metabolism_in_mutant_mice_ L2 - https://dx.doi.org/10.1007/s00335-001-3054-2 DB - PRIME DP - Unbound Medicine ER -