Tags

Type your tag names separated by a space and hit enter

Inositol lipid binding and membrane localization of isolated pleckstrin homology (PH) domains. Studies on the PH domains of phospholipase C delta 1 and p130.
J Biol Chem. 2002 Jul 26; 277(30):27412-22.JB

Abstract

The relationship between the ability of isolated pleckstrin homology (PH) domains to bind inositol lipids or soluble inositol phosphates in vitro and to localize to cellular membranes in live cells was examined by comparing the PH domains of phospholipase Cdelta(1) (PLCdelta(1)) and the recently cloned PLC-like protein p130 fused to the green fluorescent protein (GFP). The prominent membrane localization of PLCdelta(1)PH-GFP was paralleled with high affinity binding to inositol 1,4,5-trisphosphate (InsP(3)) as well as to phosphatidylinositol 4,5-bisphosphate-containing lipid vesicles or nitrocellulose membrane strips. In contrast, no membrane localization was observed with p130PH-GFP despite its InsP(3) and phosphatidylinositol 4,5-bisphosphate-binding properties being comparable with those of PLCdelta(1)PH-GFP. The N-terminal ligand binding domain of the type I InsP(3) receptor also failed to localize to the plasma membrane despite its 5-fold higher affinity to InsP(3) than the PH domains. By using a chimeric approach and cassette mutagenesis, the C-terminal alpha-helix and the short loop between the beta6-beta7 sheets of the PLCdelta(1)PH domain, in addition to its InsP(3)-binding region, were identified as critical components for membrane localization in intact cells. These data indicate that binding to the inositol phosphate head group is necessary but may not be sufficient for membrane localization of the PLCdelta(1)PH-GFP fusion protein, and motifs located within the C-terminal half of the PH domain provide auxiliary contacts with additional membrane components.

Authors+Show Affiliations

Endocrinology and Reproduction Research Branch, NICHD/National Institutes of Health, 49 Convent Drive, Bldg. 49, Bethesda, MD 20892, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12019260

Citation

Várnai, Péter, et al. "Inositol Lipid Binding and Membrane Localization of Isolated Pleckstrin Homology (PH) Domains. Studies On the PH Domains of Phospholipase C Delta 1 and P130." The Journal of Biological Chemistry, vol. 277, no. 30, 2002, pp. 27412-22.
Várnai P, Lin X, Lee SB, et al. Inositol lipid binding and membrane localization of isolated pleckstrin homology (PH) domains. Studies on the PH domains of phospholipase C delta 1 and p130. J Biol Chem. 2002;277(30):27412-22.
Várnai, P., Lin, X., Lee, S. B., Tuymetova, G., Bondeva, T., Spät, A., Rhee, S. G., Hajnóczky, G., & Balla, T. (2002). Inositol lipid binding and membrane localization of isolated pleckstrin homology (PH) domains. Studies on the PH domains of phospholipase C delta 1 and p130. The Journal of Biological Chemistry, 277(30), 27412-22.
Várnai P, et al. Inositol Lipid Binding and Membrane Localization of Isolated Pleckstrin Homology (PH) Domains. Studies On the PH Domains of Phospholipase C Delta 1 and P130. J Biol Chem. 2002 Jul 26;277(30):27412-22. PubMed PMID: 12019260.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inositol lipid binding and membrane localization of isolated pleckstrin homology (PH) domains. Studies on the PH domains of phospholipase C delta 1 and p130. AU - Várnai,Péter, AU - Lin,Xuena, AU - Lee,Sang Bong, AU - Tuymetova,Galina, AU - Bondeva,Tzvetanka, AU - Spät,Andras, AU - Rhee,Sue Goo, AU - Hajnóczky,György, AU - Balla,Tamas, Y1 - 2002/05/17/ PY - 2002/5/23/pubmed PY - 2002/8/23/medline PY - 2002/5/23/entrez SP - 27412 EP - 22 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 277 IS - 30 N2 - The relationship between the ability of isolated pleckstrin homology (PH) domains to bind inositol lipids or soluble inositol phosphates in vitro and to localize to cellular membranes in live cells was examined by comparing the PH domains of phospholipase Cdelta(1) (PLCdelta(1)) and the recently cloned PLC-like protein p130 fused to the green fluorescent protein (GFP). The prominent membrane localization of PLCdelta(1)PH-GFP was paralleled with high affinity binding to inositol 1,4,5-trisphosphate (InsP(3)) as well as to phosphatidylinositol 4,5-bisphosphate-containing lipid vesicles or nitrocellulose membrane strips. In contrast, no membrane localization was observed with p130PH-GFP despite its InsP(3) and phosphatidylinositol 4,5-bisphosphate-binding properties being comparable with those of PLCdelta(1)PH-GFP. The N-terminal ligand binding domain of the type I InsP(3) receptor also failed to localize to the plasma membrane despite its 5-fold higher affinity to InsP(3) than the PH domains. By using a chimeric approach and cassette mutagenesis, the C-terminal alpha-helix and the short loop between the beta6-beta7 sheets of the PLCdelta(1)PH domain, in addition to its InsP(3)-binding region, were identified as critical components for membrane localization in intact cells. These data indicate that binding to the inositol phosphate head group is necessary but may not be sufficient for membrane localization of the PLCdelta(1)PH-GFP fusion protein, and motifs located within the C-terminal half of the PH domain provide auxiliary contacts with additional membrane components. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/12019260/Inositol_lipid_binding_and_membrane_localization_of_isolated_pleckstrin_homology__PH__domains__Studies_on_the_PH_domains_of_phospholipase_C_delta_1_and_p130_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=12019260 DB - PRIME DP - Unbound Medicine ER -