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Nitric oxide donor stimulated increase of cyclic GMP in the goldfish retina.
Vis Neurosci. 2001 Nov-Dec; 18(6):849-56.VN

Abstract

Nitric oxide (NO) activates soluble guanylyl cyclase (sGC) and the resulting increase in cyclic guanosine monophosphate (cGMP) is an important intracellular signalling pathway in the vertebrate retina. Immunocytochemical detection of cGMP following exposure to NO donors has proven an effective method of identifying cells that express sGC. While such an approach has proven useful for the study of several vertebrate retinas, it has not been applied to the well-characterized teleost retina. Therefore, in the present study, we have applied this approach to the retina of the goldfish (Carassius auratus). In the presence of the phosphodiesterase (PDE) inhibitor 3-isobutyl-1-methylxanthine (IBMX), incubation of goldfish eyecups in Ringer's solution containing (+/-)-S-nitroso-N-acetylpenicillamine (SNAP) increased cGMP-like immunoreactivity (cG-ir) in bipolar, horizontal, amacrine, and ganglion cells and in ganglion cell axons and optic nerve. Weak labeling was observed in horizontal cells but no change in cG-ir was noted within photoreceptors. The NO donor-stimulated increases of cG-ir in horizontal, bipolar, amacrine, and ganglion cells are consistent with known physiological effects of NO on these neurons. The physiological significance of NO action at the level of optic nerve is not known. The lack of an effect of SNAP on cG-ir in photoreceptors was unexpected, as there are known physiological actions of NO, mediated by cGMP, on these neurons. Although this may be due to insufficient sensitivity of immunolabeling, this result may indicate a difference between isoforms of sGC or cGMP PDE in these neurons, compared to neurons where exogenous NO increased cG-ir.

Authors+Show Affiliations

Department of Anatomy, Dalhousie University, Halifax, Nova Scotia, Canada. wbaldrid@is.dal.caNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12020075

Citation

Baldridge, W H., and A J. Fischer. "Nitric Oxide Donor Stimulated Increase of Cyclic GMP in the Goldfish Retina." Visual Neuroscience, vol. 18, no. 6, 2001, pp. 849-56.
Baldridge WH, Fischer AJ. Nitric oxide donor stimulated increase of cyclic GMP in the goldfish retina. Vis Neurosci. 2001;18(6):849-56.
Baldridge, W. H., & Fischer, A. J. (2001). Nitric oxide donor stimulated increase of cyclic GMP in the goldfish retina. Visual Neuroscience, 18(6), 849-56.
Baldridge WH, Fischer AJ. Nitric Oxide Donor Stimulated Increase of Cyclic GMP in the Goldfish Retina. Vis Neurosci. 2001 Nov-Dec;18(6):849-56. PubMed PMID: 12020075.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nitric oxide donor stimulated increase of cyclic GMP in the goldfish retina. AU - Baldridge,W H, AU - Fischer,A J, PY - 2002/5/22/pubmed PY - 2002/7/9/medline PY - 2002/5/22/entrez SP - 849 EP - 56 JF - Visual neuroscience JO - Vis Neurosci VL - 18 IS - 6 N2 - Nitric oxide (NO) activates soluble guanylyl cyclase (sGC) and the resulting increase in cyclic guanosine monophosphate (cGMP) is an important intracellular signalling pathway in the vertebrate retina. Immunocytochemical detection of cGMP following exposure to NO donors has proven an effective method of identifying cells that express sGC. While such an approach has proven useful for the study of several vertebrate retinas, it has not been applied to the well-characterized teleost retina. Therefore, in the present study, we have applied this approach to the retina of the goldfish (Carassius auratus). In the presence of the phosphodiesterase (PDE) inhibitor 3-isobutyl-1-methylxanthine (IBMX), incubation of goldfish eyecups in Ringer's solution containing (+/-)-S-nitroso-N-acetylpenicillamine (SNAP) increased cGMP-like immunoreactivity (cG-ir) in bipolar, horizontal, amacrine, and ganglion cells and in ganglion cell axons and optic nerve. Weak labeling was observed in horizontal cells but no change in cG-ir was noted within photoreceptors. The NO donor-stimulated increases of cG-ir in horizontal, bipolar, amacrine, and ganglion cells are consistent with known physiological effects of NO on these neurons. The physiological significance of NO action at the level of optic nerve is not known. The lack of an effect of SNAP on cG-ir in photoreceptors was unexpected, as there are known physiological actions of NO, mediated by cGMP, on these neurons. Although this may be due to insufficient sensitivity of immunolabeling, this result may indicate a difference between isoforms of sGC or cGMP PDE in these neurons, compared to neurons where exogenous NO increased cG-ir. SN - 0952-5238 UR - https://www.unboundmedicine.com/medline/citation/12020075/Nitric_oxide_donor_stimulated_increase_of_cyclic_GMP_in_the_goldfish_retina_ L2 - https://www.cambridge.org/core/product/identifier/S0952523801186013/type/journal_article DB - PRIME DP - Unbound Medicine ER -