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Differential gene expression during seed germination in barley (Hordeum vulgare L.).
Funct Integr Genomics. 2002 May; 2(1-2):28-39.FI

Abstract

A barley cDNA macroarray comprising 1,440 unique genes was used to analyze the spatial and temporal patterns of gene expression in embryo, scutellum and endosperm tissue during different stages of germination. Among the set of expressed genes, 69 displayed the highest mRNA level in endosperm tissue, 58 were up-regulated in both embryo and scutellum, 11 were specifically expressed in the embryo and 16 in scutellum tissue. Based on Blast X analyses, 70% of the differentially expressed genes could be assigned a putative function. One set of genes, expressed in both embryo and scutellum tissue, included functions in cell division, protein translation, nucleotide metabolism, carbohydrate metabolism and some transporters. The other set of genes expressed in endosperm encodes several metabolic pathways including carbohydrate and amino acid metabolism as well as protease inhibitors and storage proteins. As shown for a storage protein and a trypsin inhibitor, the endosperm of the germinating barley grain contains a considerable amount of residual mRNA which was produced during seed development and which is degraded during early stages of germination. Based on similar expression patterns in the endosperm tissue, we identified 29 genes which may undergo the same degradation process.

Authors+Show Affiliations

Potokina E
Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany. potokina@ipk-gatersleben.de
Sreenivasulu N
No affiliation info available
Altschmied L
No affiliation info available
Michalek W
No affiliation info available
Graner A
No affiliation info available

MeSH

Expressed Sequence TagsGene Expression ProfilingGene Expression Regulation, PlantGerminationHordeumOligonucleotide Array Sequence AnalysisRNA, MessengerSeeds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12021848

Citation

Potokina, E, et al. "Differential Gene Expression During Seed Germination in Barley (Hordeum Vulgare L.)." Functional & Integrative Genomics, vol. 2, no. 1-2, 2002, pp. 28-39.
Potokina E, Sreenivasulu N, Altschmied L, et al. Differential gene expression during seed germination in barley (Hordeum vulgare L.). Funct Integr Genomics. 2002;2(1-2):28-39.
Potokina, E., Sreenivasulu, N., Altschmied, L., Michalek, W., & Graner, A. (2002). Differential gene expression during seed germination in barley (Hordeum vulgare L.). Functional & Integrative Genomics, 2(1-2), 28-39.
Potokina E, et al. Differential Gene Expression During Seed Germination in Barley (Hordeum Vulgare L.). Funct Integr Genomics. 2002;2(1-2):28-39. PubMed PMID: 12021848.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential gene expression during seed germination in barley (Hordeum vulgare L.). AU - Potokina,E, AU - Sreenivasulu,N, AU - Altschmied,L, AU - Michalek,W, AU - Graner,A, Y1 - 2002/03/28/ PY - 2001/12/03/received PY - 2002/01/31/accepted PY - 2002/5/22/pubmed PY - 2002/11/26/medline PY - 2002/5/22/entrez SP - 28 EP - 39 JF - Functional & integrative genomics JO - Funct Integr Genomics VL - 2 IS - 1-2 N2 - A barley cDNA macroarray comprising 1,440 unique genes was used to analyze the spatial and temporal patterns of gene expression in embryo, scutellum and endosperm tissue during different stages of germination. Among the set of expressed genes, 69 displayed the highest mRNA level in endosperm tissue, 58 were up-regulated in both embryo and scutellum, 11 were specifically expressed in the embryo and 16 in scutellum tissue. Based on Blast X analyses, 70% of the differentially expressed genes could be assigned a putative function. One set of genes, expressed in both embryo and scutellum tissue, included functions in cell division, protein translation, nucleotide metabolism, carbohydrate metabolism and some transporters. The other set of genes expressed in endosperm encodes several metabolic pathways including carbohydrate and amino acid metabolism as well as protease inhibitors and storage proteins. As shown for a storage protein and a trypsin inhibitor, the endosperm of the germinating barley grain contains a considerable amount of residual mRNA which was produced during seed development and which is degraded during early stages of germination. Based on similar expression patterns in the endosperm tissue, we identified 29 genes which may undergo the same degradation process. SN - 1438-793X UR - https://www.unboundmedicine.com/medline/citation/12021848/Differential_gene_expression_during_seed_germination_in_barley__Hordeum_vulgare_L___ DB - PRIME DP - Unbound Medicine ER -