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The soil flagellate Proleptomonas faecicola: cell organisation and phylogeny suggest that the only described free-living trypanosomatid is not a kinetoplastid but has cercomonad affinities.
Protist. 2002 Mar; 153(1):9-24.P

Abstract

The only putative free-living trypanosomatid is Proleptomonas faecicola described first by Woodcock in 1916 as a coprophilic flagellate with striking Leptomonas-like flagellar movement but lacking a kinetoplast. P faecicola was later identified by Sandon in 1927 as a widespread non-phagotrophic inhabitant of soils. No division stages were seen by either observer. An organism conforming to Woodcock's light microscope description has been isolated from tapwater and cultivated axenically in various serum-containing media. Division has been shown to occur in an aflagellate stage enclosed in a thin cyst wall. Electron microscopy of the flagellate stage reveals that, in addition to the long locomotory flagellum, a second non-motile flagellum is present attached to the body along its entire length. The flagellate's ultrastructure lacks all the major features of the Trypanosomatidae. The several mitochondria of Proleptomonas have tubular cristae and lie between intracytoplasmic microtubules originating as a loose cone associated with the flagellar basal bodies. This cytoskeleton is much reduced in the division cyst. A comparable Proleptomonas-like flagellate with similar division cysts has been observed in soil samples from farmland. Phylogenetic analysis based on SSU rRNA gene sequences suggests that the cultured organism identified here as Proleptomonas is unrelated to the Kinetoplastida and has affinities with the Phylum Cercozoa Cavalier-Smith, even though in morphology, life cycle and mode of feeding it bears little resemblance to any member of that diverse grouping.

Authors+Show Affiliations

Division of Environmental and Evolutionary Biology, University of Glasgow, UK. k.vickerman@bio.gla.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12022280

Citation

Vickerman, Keith, et al. "The Soil Flagellate Proleptomonas Faecicola: Cell Organisation and Phylogeny Suggest That the Only Described Free-living Trypanosomatid Is Not a Kinetoplastid but Has Cercomonad Affinities." Protist, vol. 153, no. 1, 2002, pp. 9-24.
Vickerman K, Le Ray D, Hoef-Emden K, et al. The soil flagellate Proleptomonas faecicola: cell organisation and phylogeny suggest that the only described free-living trypanosomatid is not a kinetoplastid but has cercomonad affinities. Protist. 2002;153(1):9-24.
Vickerman, K., Le Ray, D., Hoef-Emden, K., & De Jonckheere, J. (2002). The soil flagellate Proleptomonas faecicola: cell organisation and phylogeny suggest that the only described free-living trypanosomatid is not a kinetoplastid but has cercomonad affinities. Protist, 153(1), 9-24.
Vickerman K, et al. The Soil Flagellate Proleptomonas Faecicola: Cell Organisation and Phylogeny Suggest That the Only Described Free-living Trypanosomatid Is Not a Kinetoplastid but Has Cercomonad Affinities. Protist. 2002;153(1):9-24. PubMed PMID: 12022280.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The soil flagellate Proleptomonas faecicola: cell organisation and phylogeny suggest that the only described free-living trypanosomatid is not a kinetoplastid but has cercomonad affinities. AU - Vickerman,Keith, AU - Le Ray,Dominique, AU - Hoef-Emden,Kerstin, AU - De Jonckheere,Johan, PY - 2002/5/23/pubmed PY - 2002/12/10/medline PY - 2002/5/23/entrez SP - 9 EP - 24 JF - Protist JO - Protist VL - 153 IS - 1 N2 - The only putative free-living trypanosomatid is Proleptomonas faecicola described first by Woodcock in 1916 as a coprophilic flagellate with striking Leptomonas-like flagellar movement but lacking a kinetoplast. P faecicola was later identified by Sandon in 1927 as a widespread non-phagotrophic inhabitant of soils. No division stages were seen by either observer. An organism conforming to Woodcock's light microscope description has been isolated from tapwater and cultivated axenically in various serum-containing media. Division has been shown to occur in an aflagellate stage enclosed in a thin cyst wall. Electron microscopy of the flagellate stage reveals that, in addition to the long locomotory flagellum, a second non-motile flagellum is present attached to the body along its entire length. The flagellate's ultrastructure lacks all the major features of the Trypanosomatidae. The several mitochondria of Proleptomonas have tubular cristae and lie between intracytoplasmic microtubules originating as a loose cone associated with the flagellar basal bodies. This cytoskeleton is much reduced in the division cyst. A comparable Proleptomonas-like flagellate with similar division cysts has been observed in soil samples from farmland. Phylogenetic analysis based on SSU rRNA gene sequences suggests that the cultured organism identified here as Proleptomonas is unrelated to the Kinetoplastida and has affinities with the Phylum Cercozoa Cavalier-Smith, even though in morphology, life cycle and mode of feeding it bears little resemblance to any member of that diverse grouping. SN - 1434-4610 UR - https://www.unboundmedicine.com/medline/citation/12022280/full_citation/The_soil_flagellate_Proleptomonas_faecicola:_cell_organisation_and_phylogeny_suggest_that_the_only_described_free_living_trypanosomatid_is_not_a_kinetoplastid_but_has_cercomonad_affinities_ L2 - http://www.arb-silva.de/search/show/ssu/pubid/12022280 DB - PRIME DP - Unbound Medicine ER -