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Downregulation of P2X3 receptor-dependent sensory functions in A/J inbred mouse strain.
Eur J Neurosci 2002; 15(9):1444-50EJ

Abstract

There is large variability in the various pain responses including those to tissue injury among inbred mouse strains. However, the determinant factors for the strain-specific differences remain unknown. The P2X3 sensory-specific ATP-gated channel has been implicated as a damage-sensing molecule that evokes a pain sensation by receiving endogenous ATP from injured tissue. In this study, to clarify the contribution of the sensory P2X3 signalling to strain-specific differences in tissue injury pain, we examined whether the P2X3-mediated in vivo and in vitro responses in dorsal root ganglion (DRG) neurons are changed in the A/J inbred mouse strain, which is known to be resistant to tissue injury pain caused by formalin. Here we found that A/J mice exhibited a low magnitude of nocifensive behaviour induced by the P2X agonist alpha,beta-methylene ATP (alpha beta meATP) into the hindpaw compared with C57BL/6 J mice. This behaviour was blocked by P2X3 antisense oligodeoxynucleotides. The low magnitude of the in vivo pain sensation could be observed similarly in the in vitro response; the increase in the intracellular Ca(2+) increase by alpha beta meATP in capsaicin-sensitive DRG neurons from A/J mice was significantly lower than that from C57BL/6 J mice. In A/J DRG neurons the P2X3 protein level was significantly lower compared with C57BL/6 J DRG neurons. The change in P2X3 protein was selective because P2X2 protein was expressed equally in both strains. The present study suggests that the downregulation of sensory P2X3 could be one of the molecular predispositions to low sensitivity to tissue injury pain in the A/J inbred mouse strain.

Authors+Show Affiliations

Section of Neuropharmacology, Division of Pharmacology, National Institute of Health Sciences, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12028354

Citation

Tsuda, Makoto, et al. "Downregulation of P2X3 Receptor-dependent Sensory Functions in A/J Inbred Mouse Strain." The European Journal of Neuroscience, vol. 15, no. 9, 2002, pp. 1444-50.
Tsuda M, Shigemoto-Mogami Y, Ueno S, et al. Downregulation of P2X3 receptor-dependent sensory functions in A/J inbred mouse strain. Eur J Neurosci. 2002;15(9):1444-50.
Tsuda, M., Shigemoto-Mogami, Y., Ueno, S., Koizumi, S., Ueda, H., Iwanaga, T., & Inoue, K. (2002). Downregulation of P2X3 receptor-dependent sensory functions in A/J inbred mouse strain. The European Journal of Neuroscience, 15(9), pp. 1444-50.
Tsuda M, et al. Downregulation of P2X3 Receptor-dependent Sensory Functions in A/J Inbred Mouse Strain. Eur J Neurosci. 2002;15(9):1444-50. PubMed PMID: 12028354.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Downregulation of P2X3 receptor-dependent sensory functions in A/J inbred mouse strain. AU - Tsuda,Makoto, AU - Shigemoto-Mogami,Yukari, AU - Ueno,Shinya, AU - Koizumi,Schuichi, AU - Ueda,Hiroshi, AU - Iwanaga,Toshihiko, AU - Inoue,Kazuhide, PY - 2002/5/25/pubmed PY - 2002/7/27/medline PY - 2002/5/25/entrez SP - 1444 EP - 50 JF - The European journal of neuroscience JO - Eur. J. Neurosci. VL - 15 IS - 9 N2 - There is large variability in the various pain responses including those to tissue injury among inbred mouse strains. However, the determinant factors for the strain-specific differences remain unknown. The P2X3 sensory-specific ATP-gated channel has been implicated as a damage-sensing molecule that evokes a pain sensation by receiving endogenous ATP from injured tissue. In this study, to clarify the contribution of the sensory P2X3 signalling to strain-specific differences in tissue injury pain, we examined whether the P2X3-mediated in vivo and in vitro responses in dorsal root ganglion (DRG) neurons are changed in the A/J inbred mouse strain, which is known to be resistant to tissue injury pain caused by formalin. Here we found that A/J mice exhibited a low magnitude of nocifensive behaviour induced by the P2X agonist alpha,beta-methylene ATP (alpha beta meATP) into the hindpaw compared with C57BL/6 J mice. This behaviour was blocked by P2X3 antisense oligodeoxynucleotides. The low magnitude of the in vivo pain sensation could be observed similarly in the in vitro response; the increase in the intracellular Ca(2+) increase by alpha beta meATP in capsaicin-sensitive DRG neurons from A/J mice was significantly lower than that from C57BL/6 J mice. In A/J DRG neurons the P2X3 protein level was significantly lower compared with C57BL/6 J DRG neurons. The change in P2X3 protein was selective because P2X2 protein was expressed equally in both strains. The present study suggests that the downregulation of sensory P2X3 could be one of the molecular predispositions to low sensitivity to tissue injury pain in the A/J inbred mouse strain. SN - 0953-816X UR - https://www.unboundmedicine.com/medline/citation/12028354/Downregulation_of_P2X3_receptor_dependent_sensory_functions_in_A/J_inbred_mouse_strain_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0953-816X&date=2002&volume=15&issue=9&spage=1444 DB - PRIME DP - Unbound Medicine ER -