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Dietary fat source and cholesterol interactions alter plasma lipids and tissue susceptibility to oxidation in spontaneously hypertensive (SHR) and normotensive Wistar Kyoto (WKY) rats.
Mol Cell Biochem. 2002 Mar; 232(1-2):33-47.MC

Abstract

Due to the potential for dietary fat source to alter plasma lipids and tissue antioxidant status, we hypothesized that blends of saturated, n-6 and n-3 fats with cholesterol would affect LDL and tissue susceptibility to in vitro oxidation. The effects of dietary fat blends of butter (B), beef tallow (T), soybean oil (SBO) or menhaden oil (MO) and cholesterol on systolic blood pressure (SBP), plasma lipoproteins and tissue susceptibility to glutathione (GSH) depletion and lipid peroxidation (TBARS) were examined in spontaneously hypertensive (SHR) and Wistar Kyoto (WKY) rats. SBP in SHRs was higher (p < 0.001) than in WKYs at 13-weeks of age but was not altered by dietary fat or cholesterol. LDL- and HDL-cholesterol were greater (p < 0.001) in WKY than SHR. LDL-cholesterol and (VLDL- + LDL-cholesterol)/HDL-cholesterol ratios were reduced in MO vs. B, T and SBO groups. HDL-cholesterol levels tended to be lower and greater in B and MO groups, respectively vs. T and SBO groups. Initial LDL fluorescence was greater (p < 0.001) in high- vs. low-cholesterol groups. The change in LDL fluorescence was reduced (p < 0.001) in high-cholesterol groups, and MO vs. B, T and SBO rats. MO fed rats had reduced (p < 0.001) RBC, heart and liver GSH depletion and reduced (p < 0.01) tissue TBARS and RBC MDA production. In summary, a moderate level of dietary MO did not increase tissue and LDL in vitro oxidizability in SHR and WKY rats. High dietary cholesterol exhibited a protective effect against in vitro oxidation of LDL and selected tissues.

Authors+Show Affiliations

Food, Nutrition and Health Program, Faculty of Agricultural Sciences, University of British Columbia, Vancouver, Canada.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12030378

Citation

Yuan, Yvonne V., and David D. Kitts. "Dietary Fat Source and Cholesterol Interactions Alter Plasma Lipids and Tissue Susceptibility to Oxidation in Spontaneously Hypertensive (SHR) and Normotensive Wistar Kyoto (WKY) Rats." Molecular and Cellular Biochemistry, vol. 232, no. 1-2, 2002, pp. 33-47.
Yuan YV, Kitts DD. Dietary fat source and cholesterol interactions alter plasma lipids and tissue susceptibility to oxidation in spontaneously hypertensive (SHR) and normotensive Wistar Kyoto (WKY) rats. Mol Cell Biochem. 2002;232(1-2):33-47.
Yuan, Y. V., & Kitts, D. D. (2002). Dietary fat source and cholesterol interactions alter plasma lipids and tissue susceptibility to oxidation in spontaneously hypertensive (SHR) and normotensive Wistar Kyoto (WKY) rats. Molecular and Cellular Biochemistry, 232(1-2), 33-47.
Yuan YV, Kitts DD. Dietary Fat Source and Cholesterol Interactions Alter Plasma Lipids and Tissue Susceptibility to Oxidation in Spontaneously Hypertensive (SHR) and Normotensive Wistar Kyoto (WKY) Rats. Mol Cell Biochem. 2002;232(1-2):33-47. PubMed PMID: 12030378.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary fat source and cholesterol interactions alter plasma lipids and tissue susceptibility to oxidation in spontaneously hypertensive (SHR) and normotensive Wistar Kyoto (WKY) rats. AU - Yuan,Yvonne V, AU - Kitts,David D, PY - 2002/5/28/pubmed PY - 2003/2/7/medline PY - 2002/5/28/entrez SP - 33 EP - 47 JF - Molecular and cellular biochemistry JO - Mol Cell Biochem VL - 232 IS - 1-2 N2 - Due to the potential for dietary fat source to alter plasma lipids and tissue antioxidant status, we hypothesized that blends of saturated, n-6 and n-3 fats with cholesterol would affect LDL and tissue susceptibility to in vitro oxidation. The effects of dietary fat blends of butter (B), beef tallow (T), soybean oil (SBO) or menhaden oil (MO) and cholesterol on systolic blood pressure (SBP), plasma lipoproteins and tissue susceptibility to glutathione (GSH) depletion and lipid peroxidation (TBARS) were examined in spontaneously hypertensive (SHR) and Wistar Kyoto (WKY) rats. SBP in SHRs was higher (p < 0.001) than in WKYs at 13-weeks of age but was not altered by dietary fat or cholesterol. LDL- and HDL-cholesterol were greater (p < 0.001) in WKY than SHR. LDL-cholesterol and (VLDL- + LDL-cholesterol)/HDL-cholesterol ratios were reduced in MO vs. B, T and SBO groups. HDL-cholesterol levels tended to be lower and greater in B and MO groups, respectively vs. T and SBO groups. Initial LDL fluorescence was greater (p < 0.001) in high- vs. low-cholesterol groups. The change in LDL fluorescence was reduced (p < 0.001) in high-cholesterol groups, and MO vs. B, T and SBO rats. MO fed rats had reduced (p < 0.001) RBC, heart and liver GSH depletion and reduced (p < 0.01) tissue TBARS and RBC MDA production. In summary, a moderate level of dietary MO did not increase tissue and LDL in vitro oxidizability in SHR and WKY rats. High dietary cholesterol exhibited a protective effect against in vitro oxidation of LDL and selected tissues. SN - 0300-8177 UR - https://www.unboundmedicine.com/medline/citation/12030378/Dietary_fat_source_and_cholesterol_interactions_alter_plasma_lipids_and_tissue_susceptibility_to_oxidation_in_spontaneously_hypertensive__SHR__and_normotensive_Wistar_Kyoto__WKY__rats_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&amp;PAGE=linkout&amp;SEARCH=12030378.ui DB - PRIME DP - Unbound Medicine ER -