Tags

Type your tag names separated by a space and hit enter

The water-soluble fraction of bee venom produces antinociceptive and anti-inflammatory effects on rheumatoid arthritis in rats.

Abstract

We recently demonstrated that bee venom (BV) injection into the Zusanli acupoint produced a significantly more potent anti-inflammatory and antinociceptive effect than injection into a non-acupoint in a Freund's adjuvant induced rheumatoid arthritis (RA) model. However, the precise BV constituents responsible for these antinociceptive and/or anti-inflammatory effects are not fully understood. In order to investigate the possible role of the soluble fraction of BV in producing the anti-arthritic actions of BV acupuncture, whole BV was extracted into two fractions according to solubility (a water soluble fraction, BVA and an ethylacetate soluble fraction, BVE) and the BVA fraction was further tested. Subcutaneous BVA injection (0.9 mg/kg/day) into the Zusanli acupoint was found to dramatically inhibit paw edema and radiological change (i.e. new bone proliferation and soft tissue swelling) caused by Freund's adjuvant injection. BVA treatment also reduced the increase in serum interleukin-6 caused by RA induction to levels observed in non-arthritic animals. In addition, BVA therapy significantly reduced arthritis-induced nociceptive behaviors (i.e. nociceptive scores for mechanical hyperalgesia and thermal hyperalgesia). Finally, BVA treatment significantly suppressed adjuvant-induced Fos expression in the lumbar spinal cord at 3 weeks post-adjuvant injection. In contrast, BVE treatment (0.05 mg/kg/day) failed to show any anti-inflammatory or antinociceptive effects on RA. The results of the present study demonstrate that BVA is the effective fraction of whole BV responsible for the antinociception and anti-inflammatory effects of BV acupuncture treatment. Thus it is recommended that this fraction of BV be used for long-term treatment of RA-induced pain and inflammation. However, further study is necessary to clarify which constituents of the BVA fraction are directly responsible for these anti-arthritis effects.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Department of Veterinary Physiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon, South Korea.

    , , , , , ,

    Source

    Life sciences 71:2 2002 May 31 pg 191-204

    MeSH

    Analgesics
    Animals
    Anti-Inflammatory Agents
    Arthritis, Rheumatoid
    Bee Venoms
    Disease Models, Animal
    Hyperalgesia
    Immunohistochemistry
    Interleukin-6
    Male
    Oncogene Proteins v-fos
    Pain
    Pain Measurement
    Radiography
    Rats
    Rats, Sprague-Dawley
    Solubility
    Water

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    12031688

    Citation

    Kwon, Young Bae, et al. "The Water-soluble Fraction of Bee Venom Produces Antinociceptive and Anti-inflammatory Effects On Rheumatoid Arthritis in Rats." Life Sciences, vol. 71, no. 2, 2002, pp. 191-204.
    Kwon YB, Lee HJ, Han HJ, et al. The water-soluble fraction of bee venom produces antinociceptive and anti-inflammatory effects on rheumatoid arthritis in rats. Life Sci. 2002;71(2):191-204.
    Kwon, Y. B., Lee, H. J., Han, H. J., Mar, W. C., Kang, S. K., Yoon, O. B., ... Lee, J. H. (2002). The water-soluble fraction of bee venom produces antinociceptive and anti-inflammatory effects on rheumatoid arthritis in rats. Life Sciences, 71(2), pp. 191-204.
    Kwon YB, et al. The Water-soluble Fraction of Bee Venom Produces Antinociceptive and Anti-inflammatory Effects On Rheumatoid Arthritis in Rats. Life Sci. 2002 May 31;71(2):191-204. PubMed PMID: 12031688.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - The water-soluble fraction of bee venom produces antinociceptive and anti-inflammatory effects on rheumatoid arthritis in rats. AU - Kwon,Young Bae, AU - Lee,Hye Jung, AU - Han,Ho Jae, AU - Mar,Woung Chon, AU - Kang,Sung Keel, AU - Yoon,Ok Byung, AU - Beitz,Alvin J, AU - Lee,Jang Hern, PY - 2002/5/29/pubmed PY - 2002/7/31/medline PY - 2002/5/29/entrez SP - 191 EP - 204 JF - Life sciences JO - Life Sci. VL - 71 IS - 2 N2 - We recently demonstrated that bee venom (BV) injection into the Zusanli acupoint produced a significantly more potent anti-inflammatory and antinociceptive effect than injection into a non-acupoint in a Freund's adjuvant induced rheumatoid arthritis (RA) model. However, the precise BV constituents responsible for these antinociceptive and/or anti-inflammatory effects are not fully understood. In order to investigate the possible role of the soluble fraction of BV in producing the anti-arthritic actions of BV acupuncture, whole BV was extracted into two fractions according to solubility (a water soluble fraction, BVA and an ethylacetate soluble fraction, BVE) and the BVA fraction was further tested. Subcutaneous BVA injection (0.9 mg/kg/day) into the Zusanli acupoint was found to dramatically inhibit paw edema and radiological change (i.e. new bone proliferation and soft tissue swelling) caused by Freund's adjuvant injection. BVA treatment also reduced the increase in serum interleukin-6 caused by RA induction to levels observed in non-arthritic animals. In addition, BVA therapy significantly reduced arthritis-induced nociceptive behaviors (i.e. nociceptive scores for mechanical hyperalgesia and thermal hyperalgesia). Finally, BVA treatment significantly suppressed adjuvant-induced Fos expression in the lumbar spinal cord at 3 weeks post-adjuvant injection. In contrast, BVE treatment (0.05 mg/kg/day) failed to show any anti-inflammatory or antinociceptive effects on RA. The results of the present study demonstrate that BVA is the effective fraction of whole BV responsible for the antinociception and anti-inflammatory effects of BV acupuncture treatment. Thus it is recommended that this fraction of BV be used for long-term treatment of RA-induced pain and inflammation. However, further study is necessary to clarify which constituents of the BVA fraction are directly responsible for these anti-arthritis effects. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/12031688/The_water_soluble_fraction_of_bee_venom_produces_antinociceptive_and_anti_inflammatory_effects_on_rheumatoid_arthritis_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S002432050201617X DB - PRIME DP - Unbound Medicine ER -