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Determination of total polyphenolic content in red wines by means of the combined He-Ne laser optothermal window and Folin-Ciocalteu colorimetry assay.
Anal Chem. 2002 May 01; 74(9):2157-61.AC

Abstract

The He-Ne laser (632.8 nm) and the concept of optothermal window (OW), a variant of the open photoacoustic cell, were combined with the Folin-Ciocalteu colorimetry assay to quantitate phenolics in four red wines. The total polyphenolic content in selected red wines varied between 786 and 1630 mg/L gallic acid equivalent (GAE) as determined by OW-Folin-Ciocalteu colorimetry, which compares well to 778 and 1614 mg/L GAE obtained for the same wines by means of classical spectrophotometry. The originality and merit of OW colorimetry used here is that, unlike what is encountered in conventional spectrometry, no intermediate dilution step is required when total polyhenolics are determined in red wine. The precision, defined as the closeness to each other of 256 replicate readings of the OW signal, is generally better than 2%.

Authors+Show Affiliations

Department of Physics, University of West Hungary, Mosonmagyaróvár. dokao@mtk.nyme.huNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12033320

Citation

Dóka, Ottó, and Dane Bicanic. "Determination of Total Polyphenolic Content in Red Wines By Means of the Combined He-Ne Laser Optothermal Window and Folin-Ciocalteu Colorimetry Assay." Analytical Chemistry, vol. 74, no. 9, 2002, pp. 2157-61.
Dóka O, Bicanic D. Determination of total polyphenolic content in red wines by means of the combined He-Ne laser optothermal window and Folin-Ciocalteu colorimetry assay. Anal Chem. 2002;74(9):2157-61.
Dóka, O., & Bicanic, D. (2002). Determination of total polyphenolic content in red wines by means of the combined He-Ne laser optothermal window and Folin-Ciocalteu colorimetry assay. Analytical Chemistry, 74(9), 2157-61.
Dóka O, Bicanic D. Determination of Total Polyphenolic Content in Red Wines By Means of the Combined He-Ne Laser Optothermal Window and Folin-Ciocalteu Colorimetry Assay. Anal Chem. 2002 May 1;74(9):2157-61. PubMed PMID: 12033320.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of total polyphenolic content in red wines by means of the combined He-Ne laser optothermal window and Folin-Ciocalteu colorimetry assay. AU - Dóka,Ottó, AU - Bicanic,Dane, PY - 2002/5/30/pubmed PY - 2003/4/5/medline PY - 2002/5/30/entrez SP - 2157 EP - 61 JF - Analytical chemistry JO - Anal Chem VL - 74 IS - 9 N2 - The He-Ne laser (632.8 nm) and the concept of optothermal window (OW), a variant of the open photoacoustic cell, were combined with the Folin-Ciocalteu colorimetry assay to quantitate phenolics in four red wines. The total polyphenolic content in selected red wines varied between 786 and 1630 mg/L gallic acid equivalent (GAE) as determined by OW-Folin-Ciocalteu colorimetry, which compares well to 778 and 1614 mg/L GAE obtained for the same wines by means of classical spectrophotometry. The originality and merit of OW colorimetry used here is that, unlike what is encountered in conventional spectrometry, no intermediate dilution step is required when total polyhenolics are determined in red wine. The precision, defined as the closeness to each other of 256 replicate readings of the OW signal, is generally better than 2%. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/12033320/Determination_of_total_polyphenolic_content_in_red_wines_by_means_of_the_combined_He_Ne_laser_optothermal_window_and_Folin_Ciocalteu_colorimetry_assay_ L2 - https://doi.org/10.1021/ac011001s DB - PRIME DP - Unbound Medicine ER -
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