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Tissue transglutaminase antibody determination in celiac disease. Analysis of diagnostic specificity of anti-human IgA-type assays.
J Immunoassay Immunochem 2002; 23(2):211-27JI

Abstract

Biopsy is onerous and, for this reason, immunodiagnostics in sera of celiac disease patients are an "additional diagnostic standard." The objective of the study was to investigate the variability in diagnostic specificity of ELISAs for the detection of IgA anti-tissue transglutaminase antibodies in serum of celiac disease patients who underwent biopsy. All patients were included in the study on the basis that they had a small intestinal biopsy. We studied 18 patients with histological proven celiac disease (7 male, 11 female, mean age +/- SD: 35+/-19 years) from Graz, Austria. Healthy control subjects were also entered into the study. The determinations of the anti-tissue transglutaminase antibodies were simultaneously performed together with the endomysium and gliadin antibody markers. We analysed the 216 serum values according to Cochran's non-parametric Q-test. The complexity to the analysis reflects the complexity of the diagnostic situation with the patients. No real differences were found in the reactions of the anti-human IgA-type anti-tissue transglutaminase ELISAs. Based on these results, an association was established between the outcomes of anti-human IgA-type ELISAs for the specific antigen and patients with histologically proven celiac disease, treated for celiac disease after histology was carried out and the diagnosis was made, and healthy controls. The detection of IgA anti-tissue transglutaminase antibodies in serum is a promising alternative to the indirect immunofluorescence determination of IgA-type endomysium antibodies. One ELISA for the specific antigen showed some advantage with respect to its extended scale of detection. Immunopathology of celiac disease can be based on the results of the appropriate IgA anti-tissue transglutaminase ELISAs under uncomplicated gastrointestinal conditions.

Authors+Show Affiliations

Clinical Immunology and Jean Dausset Laboratory, Graz University M.S. and Hospital, LKH, Austria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

12033644

Citation

Földes-Papp, Zeno, et al. "Tissue Transglutaminase Antibody Determination in Celiac Disease. Analysis of Diagnostic Specificity of Anti-human IgA-type Assays." Journal of Immunoassay & Immunochemistry, vol. 23, no. 2, 2002, pp. 211-27.
Földes-Papp Z, Demel U, Berry D, et al. Tissue transglutaminase antibody determination in celiac disease. Analysis of diagnostic specificity of anti-human IgA-type assays. J Immunoassay Immunochem. 2002;23(2):211-27.
Földes-Papp, Z., Demel, U., Berry, D., Domej, W., Fuchs, D., & Tilz, G. P. (2002). Tissue transglutaminase antibody determination in celiac disease. Analysis of diagnostic specificity of anti-human IgA-type assays. Journal of Immunoassay & Immunochemistry, 23(2), pp. 211-27.
Földes-Papp Z, et al. Tissue Transglutaminase Antibody Determination in Celiac Disease. Analysis of Diagnostic Specificity of Anti-human IgA-type Assays. J Immunoassay Immunochem. 2002;23(2):211-27. PubMed PMID: 12033644.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tissue transglutaminase antibody determination in celiac disease. Analysis of diagnostic specificity of anti-human IgA-type assays. AU - Földes-Papp,Zeno, AU - Demel,Ulrike, AU - Berry,Desiree, AU - Domej,Wolfgang, AU - Fuchs,Dietmar, AU - Tilz,Gernot Peter, PY - 2002/5/30/pubmed PY - 2002/11/30/medline PY - 2002/5/30/entrez SP - 211 EP - 27 JF - Journal of immunoassay & immunochemistry JO - J Immunoassay Immunochem VL - 23 IS - 2 N2 - Biopsy is onerous and, for this reason, immunodiagnostics in sera of celiac disease patients are an "additional diagnostic standard." The objective of the study was to investigate the variability in diagnostic specificity of ELISAs for the detection of IgA anti-tissue transglutaminase antibodies in serum of celiac disease patients who underwent biopsy. All patients were included in the study on the basis that they had a small intestinal biopsy. We studied 18 patients with histological proven celiac disease (7 male, 11 female, mean age +/- SD: 35+/-19 years) from Graz, Austria. Healthy control subjects were also entered into the study. The determinations of the anti-tissue transglutaminase antibodies were simultaneously performed together with the endomysium and gliadin antibody markers. We analysed the 216 serum values according to Cochran's non-parametric Q-test. The complexity to the analysis reflects the complexity of the diagnostic situation with the patients. No real differences were found in the reactions of the anti-human IgA-type anti-tissue transglutaminase ELISAs. Based on these results, an association was established between the outcomes of anti-human IgA-type ELISAs for the specific antigen and patients with histologically proven celiac disease, treated for celiac disease after histology was carried out and the diagnosis was made, and healthy controls. The detection of IgA anti-tissue transglutaminase antibodies in serum is a promising alternative to the indirect immunofluorescence determination of IgA-type endomysium antibodies. One ELISA for the specific antigen showed some advantage with respect to its extended scale of detection. Immunopathology of celiac disease can be based on the results of the appropriate IgA anti-tissue transglutaminase ELISAs under uncomplicated gastrointestinal conditions. SN - 1532-1819 UR - https://www.unboundmedicine.com/medline/citation/12033644/Tissue_transglutaminase_antibody_determination_in_celiac_disease__Analysis_of_diagnostic_specificity_of_anti_human_IgA_type_assays_ L2 - http://www.tandfonline.com/doi/full/10.1081/IAS-120003662 DB - PRIME DP - Unbound Medicine ER -