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hrp genes of Erwinia chrysanthemi 3937 are important virulence factors.
Mol Plant Microbe Interact. 2002 May; 15(5):472-80.MP

Abstract

We developed improved virulence assays for Erwinia chrysanthemi 3937 on African violet varieties and devised a new method for the construction of precise bacterial gene knockouts. These methods were tested by constructing mutations in genes suspected to be involved with plant interactions. The virulence of the hrpG and hrcC mutant strains (both gene products presumed to be involved in protein secretion) was greatly reduced on leaves of semitolerant African violet varieties. An hrpN mutant strain produced delayed symptoms on African violet leaves and an hrpN delta pel (delta pel = five major pectate lyase genes deleted) double mutant was nonpathogenic. The hrcC and hrpG mutants did not produce a rapid hypersensitive response (HR) in tobacco, unlike the wild-type bacterium, and the hrpN mutant gave a reduced HR. The results, therefore, establish the importance of hrp genes in the virulence of E. chrysanthemi and their ability to elicit HR on nonhosts. The data also suggest that other effector proteins secreted by the Hrp system are required for full virulence and HR elicitation.

Authors+Show Affiliations

Yang CH
Department of Plant Pathology, University of California, Riverside 92521, USA.
Gavilanes-Ruiz M
No affiliation info available
Okinaka Y
No affiliation info available
Vedel R
No affiliation info available
Berthuy I
No affiliation info available
Boccara M
No affiliation info available
Chen JW
No affiliation info available
Perna NT
No affiliation info available
Keen NT
No affiliation info available

MeSH

Bacterial Outer Membrane ProteinsBacterial ProteinsCloning, MolecularDNA, BacterialDickeya chrysanthemiGenes, BacterialMagnoliopsidaMolecular Sequence DataMutationPlant DiseasesPlant LeavesSequence Analysis, DNATobaccoTranscription FactorsVirulence

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

12036278

Citation

Yang, Ching-Hong, et al. "Hrp Genes of Erwinia Chrysanthemi 3937 Are Important Virulence Factors." Molecular Plant-microbe Interactions : MPMI, vol. 15, no. 5, 2002, pp. 472-80.
Yang CH, Gavilanes-Ruiz M, Okinaka Y, et al. Hrp genes of Erwinia chrysanthemi 3937 are important virulence factors. Mol Plant Microbe Interact. 2002;15(5):472-80.
Yang, C. H., Gavilanes-Ruiz, M., Okinaka, Y., Vedel, R., Berthuy, I., Boccara, M., Chen, J. W., Perna, N. T., & Keen, N. T. (2002). Hrp genes of Erwinia chrysanthemi 3937 are important virulence factors. Molecular Plant-microbe Interactions : MPMI, 15(5), 472-80.
Yang CH, et al. Hrp Genes of Erwinia Chrysanthemi 3937 Are Important Virulence Factors. Mol Plant Microbe Interact. 2002;15(5):472-80. PubMed PMID: 12036278.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - hrp genes of Erwinia chrysanthemi 3937 are important virulence factors. AU - Yang,Ching-Hong, AU - Gavilanes-Ruiz,Marina, AU - Okinaka,Yasushi, AU - Vedel,Regine, AU - Berthuy,Isabelle, AU - Boccara,Martine, AU - Chen,Jason Wei-Ta, AU - Perna,Nicole T, AU - Keen,Noel T, PY - 2002/5/31/pubmed PY - 2003/1/8/medline PY - 2002/5/31/entrez SP - 472 EP - 80 JF - Molecular plant-microbe interactions : MPMI JO - Mol Plant Microbe Interact VL - 15 IS - 5 N2 - We developed improved virulence assays for Erwinia chrysanthemi 3937 on African violet varieties and devised a new method for the construction of precise bacterial gene knockouts. These methods were tested by constructing mutations in genes suspected to be involved with plant interactions. The virulence of the hrpG and hrcC mutant strains (both gene products presumed to be involved in protein secretion) was greatly reduced on leaves of semitolerant African violet varieties. An hrpN mutant strain produced delayed symptoms on African violet leaves and an hrpN delta pel (delta pel = five major pectate lyase genes deleted) double mutant was nonpathogenic. The hrcC and hrpG mutants did not produce a rapid hypersensitive response (HR) in tobacco, unlike the wild-type bacterium, and the hrpN mutant gave a reduced HR. The results, therefore, establish the importance of hrp genes in the virulence of E. chrysanthemi and their ability to elicit HR on nonhosts. The data also suggest that other effector proteins secreted by the Hrp system are required for full virulence and HR elicitation. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/12036278/hrp_genes_of_Erwinia_chrysanthemi_3937_are_important_virulence_factors_ DB - PRIME DP - Unbound Medicine ER -