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Synthesis of a novel class of polyhydroxyalkanoates in Arabidopsis peroxisomes, and their use in monitoring short-chain-length intermediates of beta-oxidation.
Plant Cell Physiol. 2002 May; 43(5):555-62.PC

Abstract

The poly[(R)-3-hydroxyalkanoate] (PHA) synthase gene (phaC(Ac)) of Aeromonas caviae FA440 was modified by adding a peroxisome targeting signal encoding the last 10 amino acids at the carboxyl-terminal of spinach glycolate oxidase. The modified gene was introduced into Arabidopsis thaliana plants by Agrobacterium-mediated transformation. The transgenic Arabidopsis plant expressed the introduced gene and its protein, and it accumulated PHA in its tissues. Gas chromatography-mass spectrometry analysis demonstrated the accumulation of a novel type of PHA, poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate). This strongly suggests that short-chain-length (R)-3-hydroxyacyl-CoAs were generated from intermediates of peroxisomal beta-oxidation. It was revealed by using this transgenic plant that Tween-20 can activate peroxisomal beta-oxidation of short-chain-length fatty acids.

Authors+Show Affiliations

RIKEN Institute, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198 Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

12040103

Citation

Arai, Yuko, et al. "Synthesis of a Novel Class of Polyhydroxyalkanoates in Arabidopsis Peroxisomes, and Their Use in Monitoring Short-chain-length Intermediates of Beta-oxidation." Plant & Cell Physiology, vol. 43, no. 5, 2002, pp. 555-62.
Arai Y, Nakashita H, Suzuki Y, et al. Synthesis of a novel class of polyhydroxyalkanoates in Arabidopsis peroxisomes, and their use in monitoring short-chain-length intermediates of beta-oxidation. Plant Cell Physiol. 2002;43(5):555-62.
Arai, Y., Nakashita, H., Suzuki, Y., Kobayashi, Y., Shimizu, T., Yasuda, M., Doi, Y., & Yamaguchi, I. (2002). Synthesis of a novel class of polyhydroxyalkanoates in Arabidopsis peroxisomes, and their use in monitoring short-chain-length intermediates of beta-oxidation. Plant & Cell Physiology, 43(5), 555-62.
Arai Y, et al. Synthesis of a Novel Class of Polyhydroxyalkanoates in Arabidopsis Peroxisomes, and Their Use in Monitoring Short-chain-length Intermediates of Beta-oxidation. Plant Cell Physiol. 2002;43(5):555-62. PubMed PMID: 12040103.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis of a novel class of polyhydroxyalkanoates in Arabidopsis peroxisomes, and their use in monitoring short-chain-length intermediates of beta-oxidation. AU - Arai,Yuko, AU - Nakashita,Hideo, AU - Suzuki,Yoshikatu, AU - Kobayashi,Yumiko, AU - Shimizu,Toshiyuki, AU - Yasuda,Michiko, AU - Doi,Yoshiharu, AU - Yamaguchi,Isamu, PY - 2002/6/1/pubmed PY - 2002/8/21/medline PY - 2002/6/1/entrez SP - 555 EP - 62 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 43 IS - 5 N2 - The poly[(R)-3-hydroxyalkanoate] (PHA) synthase gene (phaC(Ac)) of Aeromonas caviae FA440 was modified by adding a peroxisome targeting signal encoding the last 10 amino acids at the carboxyl-terminal of spinach glycolate oxidase. The modified gene was introduced into Arabidopsis thaliana plants by Agrobacterium-mediated transformation. The transgenic Arabidopsis plant expressed the introduced gene and its protein, and it accumulated PHA in its tissues. Gas chromatography-mass spectrometry analysis demonstrated the accumulation of a novel type of PHA, poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate). This strongly suggests that short-chain-length (R)-3-hydroxyacyl-CoAs were generated from intermediates of peroxisomal beta-oxidation. It was revealed by using this transgenic plant that Tween-20 can activate peroxisomal beta-oxidation of short-chain-length fatty acids. SN - 0032-0781 UR - https://www.unboundmedicine.com/medline/citation/12040103/Synthesis_of_a_novel_class_of_polyhydroxyalkanoates_in_Arabidopsis_peroxisomes_and_their_use_in_monitoring_short_chain_length_intermediates_of_beta_oxidation_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcf068 DB - PRIME DP - Unbound Medicine ER -