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Molecular identification of food-borne and water-borne protozoa.
Southeast Asian J Trop Med Public Health. 2001; 32 Suppl 2:156-8.SA

Abstract

Cryptosporidium and Giardia can be transmitted to humans by contaminated food and water, resulting in large outbreaks of diarrheal disease. Sensitive methods for detecting these parasites are needed to control and prevent infection. However, this issue is complicated by the fact that there is still uncertainty about the role played by different species/genotypes with respect to human disease. We are in the process of collecting samples from clinical cases (both sporadic and outbreak-related human infections) and from the environment (tap and waste water samples from different geographic regions), to test the efficacy of methods for detection and genotyping. Concerning Cryptosporidium parvum, we have developed new genotyping methods based on highly polymorphic microsatellite markers. The use of microsatellite markers allows the route of transmission to be traced; these methods can also be used not only to distinguish between anthroponotic and zoonotic transmission but also to identify the source(s) of infection. Regarding Giardia, which was found very frequently in environmental water samples, we are testing the beta-giardin gene as a marker to discriminate among species/genotypes.

Authors+Show Affiliations

Cacciò S
Laboratory of Parasitology, Istituto Superiore Sanità, Rome, Italy.
Pozio E
No affiliation info available

MeSH

AnimalsBase SequenceCryptosporidiosisCryptosporidiumDNA, ProtozoanDisease OutbreaksFood ParasitologyGenotypeGiardiaGiardiasisHumansMicrosatellite RepeatsPolymerase Chain ReactionSequence AlignmentSequence Homology, Nucleic AcidSpecies SpecificityWater

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12041581

Citation

Cacciò, S, and E Pozio. "Molecular Identification of Food-borne and Water-borne Protozoa." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 32 Suppl 2, 2001, pp. 156-8.
Cacciò S, Pozio E. Molecular identification of food-borne and water-borne protozoa. Southeast Asian J Trop Med Public Health. 2001;32 Suppl 2:156-8.
Cacciò, S., & Pozio, E. (2001). Molecular identification of food-borne and water-borne protozoa. The Southeast Asian Journal of Tropical Medicine and Public Health, 32 Suppl 2, 156-8.
Cacciò S, Pozio E. Molecular Identification of Food-borne and Water-borne Protozoa. Southeast Asian J Trop Med Public Health. 2001;32 Suppl 2:156-8. PubMed PMID: 12041581.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular identification of food-borne and water-borne protozoa. AU - Cacciò,S, AU - Pozio,E, PY - 2002/6/4/pubmed PY - 2002/8/17/medline PY - 2002/6/4/entrez SP - 156 EP - 8 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J Trop Med Public Health VL - 32 Suppl 2 N2 - Cryptosporidium and Giardia can be transmitted to humans by contaminated food and water, resulting in large outbreaks of diarrheal disease. Sensitive methods for detecting these parasites are needed to control and prevent infection. However, this issue is complicated by the fact that there is still uncertainty about the role played by different species/genotypes with respect to human disease. We are in the process of collecting samples from clinical cases (both sporadic and outbreak-related human infections) and from the environment (tap and waste water samples from different geographic regions), to test the efficacy of methods for detection and genotyping. Concerning Cryptosporidium parvum, we have developed new genotyping methods based on highly polymorphic microsatellite markers. The use of microsatellite markers allows the route of transmission to be traced; these methods can also be used not only to distinguish between anthroponotic and zoonotic transmission but also to identify the source(s) of infection. Regarding Giardia, which was found very frequently in environmental water samples, we are testing the beta-giardin gene as a marker to discriminate among species/genotypes. SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/12041581/Molecular_identification_of_food_borne_and_water_borne_protozoa_ L2 - https://medlineplus.gov/cryptosporidiosis.html DB - PRIME DP - Unbound Medicine ER -