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[Generation and characterization of chondrocyte specific Cre transgenic mice].
Yi Chuan Xue Bao. 2002 May; 29(5):424-9.YC

Abstract

A chondrocyte specific transgenic construct (pcol2A1-Cre) containing the cartilage specific type II collagen A1 promoter, the Cre recombinase gene and the polyA of human growth factor gene was generated. The 9.3 kb DNA fragments were recovered from Not I digested fragments and microinjected into 323 fertilized eggs. The injected eggs were implanted into the oviduct of 14 female mice. In the 52 offsprings, there were 10 mice carring the transgene identified by PCR, and the efficiency was 19.2%. The col2A1-Cre transgenic mice were crossed with a conditional gene targeting mice to check the Cre mediated recombination in multiple tissues. The results of the PCR analysis suggested that the Cre recombinase was expressed only in cartilaginous tissue and could mediate the recombination between the LoxP sites in vivo. The result was further confirmed by Southern-blot.

Authors+Show Affiliations

Genetic Laboratory of Development and Diseases, Institute of Biotechnology Academy of Military Medical Sciences, Beijing 100071, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12043570

Citation

Hao, Zhen-Ming, et al. "[Generation and Characterization of Chondrocyte Specific Cre Transgenic Mice]." Yi Chuan Xue Bao = Acta Genetica Sinica, vol. 29, no. 5, 2002, pp. 424-9.
Hao ZM, Yang X, Cheng X, et al. [Generation and characterization of chondrocyte specific Cre transgenic mice]. Yi Chuan Xue Bao. 2002;29(5):424-9.
Hao, Z. M., Yang, X., Cheng, X., Zhou, J., & Huang, C. F. (2002). [Generation and characterization of chondrocyte specific Cre transgenic mice]. Yi Chuan Xue Bao = Acta Genetica Sinica, 29(5), 424-9.
Hao ZM, et al. [Generation and Characterization of Chondrocyte Specific Cre Transgenic Mice]. Yi Chuan Xue Bao. 2002;29(5):424-9. PubMed PMID: 12043570.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Generation and characterization of chondrocyte specific Cre transgenic mice]. AU - Hao,Zhen-Ming, AU - Yang,Xiao, AU - Cheng,Xuan, AU - Zhou,Jiang, AU - Huang,Cui-Fen, PY - 2002/6/5/pubmed PY - 2002/7/4/medline PY - 2002/6/5/entrez SP - 424 EP - 9 JF - Yi chuan xue bao = Acta genetica Sinica JO - Yi Chuan Xue Bao VL - 29 IS - 5 N2 - A chondrocyte specific transgenic construct (pcol2A1-Cre) containing the cartilage specific type II collagen A1 promoter, the Cre recombinase gene and the polyA of human growth factor gene was generated. The 9.3 kb DNA fragments were recovered from Not I digested fragments and microinjected into 323 fertilized eggs. The injected eggs were implanted into the oviduct of 14 female mice. In the 52 offsprings, there were 10 mice carring the transgene identified by PCR, and the efficiency was 19.2%. The col2A1-Cre transgenic mice were crossed with a conditional gene targeting mice to check the Cre mediated recombination in multiple tissues. The results of the PCR analysis suggested that the Cre recombinase was expressed only in cartilaginous tissue and could mediate the recombination between the LoxP sites in vivo. The result was further confirmed by Southern-blot. SN - 0379-4172 UR - https://www.unboundmedicine.com/medline/citation/12043570/[Generation_and_characterization_of_chondrocyte_specific_Cre_transgenic_mice]_ L2 - http://www.komp.org/ncbi.php?PMID=12043570 DB - PRIME DP - Unbound Medicine ER -