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Uvitex 2B stain for the diagnosis of Isospora belli infections in patients with the acquired immunodeficiency syndrome.
Arch Pathol Lab Med. 1996 Nov; 120(11):1023-5.AP

Abstract

OBJECTIVE

Fluorescent stains with Uvitex 2B or other fluorochromes are widely used today, especially for the diagnosis of microsporidian infections in human immunodeficiency virus (HIV)-infected patients. Our objective was to ascertain whether the fluorescent stain with Uvitex 2B is also able to detect Isospora belli in stool samples and duodenal/bile juice of HIV-infected patients.

DESIGN

Case study.

SETTING

University hospital of the University of Cologne, Germany.

PATIENTS

Two HIV-infected patients with chronic diarrhea who had intestinal infections with I. belli.

METHODS

Stool was concentrated by a modified water-ether sedimentation method, and duodenal/bile juice was concentrated by centrifugation. Wet-mount preparations were examined by phase-contrast and bright-field microscopy, and smears were stained with a modified acid-fast stain and a fluorescent stain with Uvitex 2B.

RESULTS

Using the fluorescent stain with Uvitex 2B, the oocysts of I. belli stained bright white/blue fluorescent and showed a structure similar to that of the oocysts in acidfast stains.

CONCLUSIONS

Staining with Uvitex 2B is a suitable method for the diagnosis of I. belli infections. This technique can be used for the diagnosis of three important gastrointestinal parasites (viz, microsporidia, cryptosporidia, and I. belli) responsible for diarrhea in HIV-infected patients.

Authors+Show Affiliations

Department of Internal Medicine I, University of Cologne, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

12049103

Citation

Franzen, C, et al. "Uvitex 2B Stain for the Diagnosis of Isospora Belli Infections in Patients With the Acquired Immunodeficiency Syndrome." Archives of Pathology & Laboratory Medicine, vol. 120, no. 11, 1996, pp. 1023-5.
Franzen C, Müller A, Salzberger B, et al. Uvitex 2B stain for the diagnosis of Isospora belli infections in patients with the acquired immunodeficiency syndrome. Arch Pathol Lab Med. 1996;120(11):1023-5.
Franzen, C., Müller, A., Salzberger, B., Hartmann, P., Diehl, V., & Fätkenheuer, G. (1996). Uvitex 2B stain for the diagnosis of Isospora belli infections in patients with the acquired immunodeficiency syndrome. Archives of Pathology & Laboratory Medicine, 120(11), 1023-5.
Franzen C, et al. Uvitex 2B Stain for the Diagnosis of Isospora Belli Infections in Patients With the Acquired Immunodeficiency Syndrome. Arch Pathol Lab Med. 1996;120(11):1023-5. PubMed PMID: 12049103.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Uvitex 2B stain for the diagnosis of Isospora belli infections in patients with the acquired immunodeficiency syndrome. AU - Franzen,C, AU - Müller,A, AU - Salzberger,B, AU - Hartmann,P, AU - Diehl,V, AU - Fätkenheuer,G, PY - 1996/11/1/pubmed PY - 2002/7/27/medline PY - 1996/11/1/entrez SP - 1023 EP - 5 JF - Archives of pathology & laboratory medicine JO - Arch Pathol Lab Med VL - 120 IS - 11 N2 - OBJECTIVE: Fluorescent stains with Uvitex 2B or other fluorochromes are widely used today, especially for the diagnosis of microsporidian infections in human immunodeficiency virus (HIV)-infected patients. Our objective was to ascertain whether the fluorescent stain with Uvitex 2B is also able to detect Isospora belli in stool samples and duodenal/bile juice of HIV-infected patients. DESIGN: Case study. SETTING: University hospital of the University of Cologne, Germany. PATIENTS: Two HIV-infected patients with chronic diarrhea who had intestinal infections with I. belli. METHODS: Stool was concentrated by a modified water-ether sedimentation method, and duodenal/bile juice was concentrated by centrifugation. Wet-mount preparations were examined by phase-contrast and bright-field microscopy, and smears were stained with a modified acid-fast stain and a fluorescent stain with Uvitex 2B. RESULTS: Using the fluorescent stain with Uvitex 2B, the oocysts of I. belli stained bright white/blue fluorescent and showed a structure similar to that of the oocysts in acidfast stains. CONCLUSIONS: Staining with Uvitex 2B is a suitable method for the diagnosis of I. belli infections. This technique can be used for the diagnosis of three important gastrointestinal parasites (viz, microsporidia, cryptosporidia, and I. belli) responsible for diarrhea in HIV-infected patients. SN - 0003-9985 UR - https://www.unboundmedicine.com/medline/citation/12049103/Uvitex_2B_stain_for_the_diagnosis_of_Isospora_belli_infections_in_patients_with_the_acquired_immunodeficiency_syndrome_ L2 - https://medlineplus.gov/hivaidsandinfections.html DB - PRIME DP - Unbound Medicine ER -