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Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting.
Biochem Biophys Res Commun. 2002 May 10; 293(3):953-7.BB

Abstract

We constructed an expression vector of Flp recombinase modified by adding a nuclear localization signal. Injection of the expression vector into fertilized eggs of the C57BL/6 strain yielded transgenic mouse lines expressing the Flp recombinase transgene in the testis. We crossed the transgenic mice to reporter mice carrying the neomycin phosphotransferase gene flanked by target sites of Flp recombinase. Examination of the deletion of the neomycin phosphotransferase gene in the progeny showed that Flp-mediated recombination took place efficiently in vivo in FLP66 transgenic mouse line. These results suggest that the Flp recombinase system is effective in mice and in combination with the Cre recombinase system extends the potentials of gene manipulation in mice. One of the useful applications of FLP66 transgenic mouse line is the removal of marker genes from mice manipulated for the conditional gene targeting with the Cre/loxP system in the pure C57BL/6 genetic background.

Authors+Show Affiliations

Department of Molecular Neurobiology and Pharmacology, Graduate School of Medicine, University of Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12051751

Citation

Takeuchi, Tomonori, et al. "Flp Recombinase Transgenic Mice of C57BL/6 Strain for Conditional Gene Targeting." Biochemical and Biophysical Research Communications, vol. 293, no. 3, 2002, pp. 953-7.
Takeuchi T, Nomura T, Tsujita M, et al. Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting. Biochem Biophys Res Commun. 2002;293(3):953-7.
Takeuchi, T., Nomura, T., Tsujita, M., Suzuki, M., Fuse, T., Mori, H., & Mishina, M. (2002). Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting. Biochemical and Biophysical Research Communications, 293(3), 953-7.
Takeuchi T, et al. Flp Recombinase Transgenic Mice of C57BL/6 Strain for Conditional Gene Targeting. Biochem Biophys Res Commun. 2002 May 10;293(3):953-7. PubMed PMID: 12051751.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting. AU - Takeuchi,Tomonori, AU - Nomura,Takanori, AU - Tsujita,Mika, AU - Suzuki,Misao, AU - Fuse,Toshimitsu, AU - Mori,Hisashi, AU - Mishina,Masayoshi, PY - 2002/6/8/pubmed PY - 2002/6/28/medline PY - 2002/6/8/entrez SP - 953 EP - 7 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 293 IS - 3 N2 - We constructed an expression vector of Flp recombinase modified by adding a nuclear localization signal. Injection of the expression vector into fertilized eggs of the C57BL/6 strain yielded transgenic mouse lines expressing the Flp recombinase transgene in the testis. We crossed the transgenic mice to reporter mice carrying the neomycin phosphotransferase gene flanked by target sites of Flp recombinase. Examination of the deletion of the neomycin phosphotransferase gene in the progeny showed that Flp-mediated recombination took place efficiently in vivo in FLP66 transgenic mouse line. These results suggest that the Flp recombinase system is effective in mice and in combination with the Cre recombinase system extends the potentials of gene manipulation in mice. One of the useful applications of FLP66 transgenic mouse line is the removal of marker genes from mice manipulated for the conditional gene targeting with the Cre/loxP system in the pure C57BL/6 genetic background. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/12051751/Flp_recombinase_transgenic_mice_of_C57BL/6_strain_for_conditional_gene_targeting_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(02)00321-2 DB - PRIME DP - Unbound Medicine ER -