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Direct amperometric determination of lactate at a carbon fiber bundle microdisk electrode by capillary zone electrophoresis.
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Jul 05; 774(1):121-6.JC

Abstract

Capillary zone electrophoresis was employed for the determination of lactate using end-column amperometric detection at a carbon fiber bundle microdisk electrode. The optimum conditions of separation and detection are 3.6 x 10(-3) mol/l Na(2)HPO(4)-1.4 x 10(-3) mol/l NaH(2)PO (pH 7.2) for the buffer solution, 18 kV for the separation voltage and 1.60 V versus the saturated calomel electrode for the detection potential. The limit of detection is 7.6 x 10(-7) mol/l or 1.7 fmol (S/N=3) and the linear range is 1.7 x 10(-6)-8.2 x 10(-4) mol/l for the injection voltage of 6 kV and injection time of 5 s. The RSD is 1.8% for the migration time and 3.3% for the electrophoretic peak current. The method was applied to the determination of lactate in human saliva. The recovery of the method is between 95 and 109%.

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Authors+Show Affiliations

Dong Q
School of Chemistry and Chemical Engineering, and State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Dong R
No affiliation info available
Jin M
No affiliation info available
Jin W
No affiliation info available

MeSH

CarbonElectrodesElectrophoresis, CapillaryHumansLactic AcidReproducibility of ResultsSalivaSensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12052730

Citation

Dong, Qian, et al. "Direct Amperometric Determination of Lactate at a Carbon Fiber Bundle Microdisk Electrode By Capillary Zone Electrophoresis." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 774, no. 1, 2002, pp. 121-6.
Dong Q, Dong R, Jin M, et al. Direct amperometric determination of lactate at a carbon fiber bundle microdisk electrode by capillary zone electrophoresis. J Chromatogr B Analyt Technol Biomed Life Sci. 2002;774(1):121-6.
Dong, Q., Dong, R., Jin, M., & Jin, W. (2002). Direct amperometric determination of lactate at a carbon fiber bundle microdisk electrode by capillary zone electrophoresis. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 774(1), 121-6.
Dong Q, et al. Direct Amperometric Determination of Lactate at a Carbon Fiber Bundle Microdisk Electrode By Capillary Zone Electrophoresis. J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Jul 5;774(1):121-6. PubMed PMID: 12052730.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct amperometric determination of lactate at a carbon fiber bundle microdisk electrode by capillary zone electrophoresis. AU - Dong,Qian, AU - Dong,Rui, AU - Jin,Mingliang, AU - Jin,Wenrui, PY - 2002/6/8/pubmed PY - 2002/8/23/medline PY - 2002/6/8/entrez SP - 121 EP - 6 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 774 IS - 1 N2 - Capillary zone electrophoresis was employed for the determination of lactate using end-column amperometric detection at a carbon fiber bundle microdisk electrode. The optimum conditions of separation and detection are 3.6 x 10(-3) mol/l Na(2)HPO(4)-1.4 x 10(-3) mol/l NaH(2)PO (pH 7.2) for the buffer solution, 18 kV for the separation voltage and 1.60 V versus the saturated calomel electrode for the detection potential. The limit of detection is 7.6 x 10(-7) mol/l or 1.7 fmol (S/N=3) and the linear range is 1.7 x 10(-6)-8.2 x 10(-4) mol/l for the injection voltage of 6 kV and injection time of 5 s. The RSD is 1.8% for the migration time and 3.3% for the electrophoretic peak current. The method was applied to the determination of lactate in human saliva. The recovery of the method is between 95 and 109%. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/12052730/Direct_amperometric_determination_of_lactate_at_a_carbon_fiber_bundle_microdisk_electrode_by_capillary_zone_electrophoresis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570023202001861 DB - PRIME DP - Unbound Medicine ER -