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VEGF and VEGF receptor levels in retinal and brain-derived endothelial cells.
Biochem Biophys Res Commun. 2002 May 03; 293(2):710-3.BB

Abstract

Vascular endothelial cell growth factor (VEGF) is an endothelial cell-specific angiogenic and permeability-inducing factor that has been implicated in the pathogenesis of diabetic retinopathy. The objectives of this study are to compare VEGF and VEGF receptor expression between retinal and brain-derived endothelial cells cultured in 5 or 30 mM glucose for 5 days. Our results show that expression of cell-surface VEGF receptors, assessed by flow cytometry, is higher in retinal-derived endothelial cells. RT-PCR results show that both retinal and brain-derived endothelial cells express comparable levels and types of VEGF. Exposure to 30 mM glucose for 5 days did not alter levels of VEGF or VEGF receptors. The higher level of VEGF receptor expression in retinal endothelial cells suggests that the retinal microcirculation may be more sensitive to the effects of VEGF and this may contribute to the pathogenesis of diabetic retinopathy.

Authors+Show Affiliations

Department of Pathology and the Oklahoma Center for Neuroscience, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12054527

Citation

Rajah, Talitha T., and Paula Grammas. "VEGF and VEGF Receptor Levels in Retinal and Brain-derived Endothelial Cells." Biochemical and Biophysical Research Communications, vol. 293, no. 2, 2002, pp. 710-3.
Rajah TT, Grammas P. VEGF and VEGF receptor levels in retinal and brain-derived endothelial cells. Biochem Biophys Res Commun. 2002;293(2):710-3.
Rajah, T. T., & Grammas, P. (2002). VEGF and VEGF receptor levels in retinal and brain-derived endothelial cells. Biochemical and Biophysical Research Communications, 293(2), 710-3.
Rajah TT, Grammas P. VEGF and VEGF Receptor Levels in Retinal and Brain-derived Endothelial Cells. Biochem Biophys Res Commun. 2002 May 3;293(2):710-3. PubMed PMID: 12054527.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - VEGF and VEGF receptor levels in retinal and brain-derived endothelial cells. AU - Rajah,Talitha T, AU - Grammas,Paula, PY - 2002/6/11/pubmed PY - 2002/7/13/medline PY - 2002/6/11/entrez SP - 710 EP - 3 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 293 IS - 2 N2 - Vascular endothelial cell growth factor (VEGF) is an endothelial cell-specific angiogenic and permeability-inducing factor that has been implicated in the pathogenesis of diabetic retinopathy. The objectives of this study are to compare VEGF and VEGF receptor expression between retinal and brain-derived endothelial cells cultured in 5 or 30 mM glucose for 5 days. Our results show that expression of cell-surface VEGF receptors, assessed by flow cytometry, is higher in retinal-derived endothelial cells. RT-PCR results show that both retinal and brain-derived endothelial cells express comparable levels and types of VEGF. Exposure to 30 mM glucose for 5 days did not alter levels of VEGF or VEGF receptors. The higher level of VEGF receptor expression in retinal endothelial cells suggests that the retinal microcirculation may be more sensitive to the effects of VEGF and this may contribute to the pathogenesis of diabetic retinopathy. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/12054527/VEGF_and_VEGF_receptor_levels_in_retinal_and_brain_derived_endothelial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(02)00281-4 DB - PRIME DP - Unbound Medicine ER -