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Eight-hydroxy-2'-deoxyguanosine in granulosa cells is correlated with the quality of oocytes and embryos in an in vitro fertilization-embryo transfer program.
Fertil Steril. 2002 Jun; 77(6):1184-90.FS

Abstract

OBJECTIVE

To determine the effects of oxidative stress on the quality of oocytes and embryos, 8-hydroxy-2'-deoxyguanosine (8-OHdG) in granulosa cells was quantitatively studied during an in vitro fertilization and embryo transfer (IVF-ET) program.

DESIGN

Immunocytochemical staining of 8-OHdG in granulosa cells was quantitatively estimated using a charge-coupled device camera and analyzed using the National Institute of Health Image (NIH Image) freeware on a computer .

SETTING

Obstetrics and gynecology department in a university hospital.

PATIENT(S)

Ninety-six infertile couples undergoing IVF-ET treatment and intracytoplasmic sperm injection (IVF, n = 72; intracytoplasmic sperm injection, n = 24).

INTERVENTION(S)

Oocytes, granulosa cells, and follicular fluids were collected 35-36 hours after the administration of hCG.

MAIN OUTCOME MEASURE(S)

8-OHdG indices were obtained for mural [8-OHdG index (m)] and cumulus [8-OHdG index (c)] granulosa cells.

RESULT(S)

A negative correlation between the fertilization rate and both 8-OHdG indices (c and m) was found. The rate of production of good embryos also showed a negative correlation with the 8-OHdG index (m) and the 8-OHdG index (c). Negative correlations between the 8-OHdG index (c) and E2 levels in follicular fluid were observed. Endometriosis patients showed a higher 8-OHdG index (c) than did patients with other infertility causes, such as tubal, male factor, and unknown.

CONCLUSION(S)

Oxidative stress in granulosa cells lowered fertilization rates and subsequently led to a decrease in the quality of embryos. The quality of oocytes for endometriosis patients was impaired by the presence of 8-OHdG. This might be one causative factor in infertility in endometriosis patients.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Yamagata, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12057726

Citation

Seino, Tomofumi, et al. "Eight-hydroxy-2'-deoxyguanosine in Granulosa Cells Is Correlated With the Quality of Oocytes and Embryos in an in Vitro Fertilization-embryo Transfer Program." Fertility and Sterility, vol. 77, no. 6, 2002, pp. 1184-90.
Seino T, Saito H, Kaneko T, et al. Eight-hydroxy-2'-deoxyguanosine in granulosa cells is correlated with the quality of oocytes and embryos in an in vitro fertilization-embryo transfer program. Fertil Steril. 2002;77(6):1184-90.
Seino, T., Saito, H., Kaneko, T., Takahashi, T., Kawachiya, S., & Kurachi, H. (2002). Eight-hydroxy-2'-deoxyguanosine in granulosa cells is correlated with the quality of oocytes and embryos in an in vitro fertilization-embryo transfer program. Fertility and Sterility, 77(6), 1184-90.
Seino T, et al. Eight-hydroxy-2'-deoxyguanosine in Granulosa Cells Is Correlated With the Quality of Oocytes and Embryos in an in Vitro Fertilization-embryo Transfer Program. Fertil Steril. 2002;77(6):1184-90. PubMed PMID: 12057726.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Eight-hydroxy-2'-deoxyguanosine in granulosa cells is correlated with the quality of oocytes and embryos in an in vitro fertilization-embryo transfer program. AU - Seino,Tomofumi, AU - Saito,Hidekazu, AU - Kaneko,Tomoko, AU - Takahashi,Toshifumi, AU - Kawachiya,Satoshi, AU - Kurachi,Hirohisa, PY - 2002/6/12/pubmed PY - 2002/7/4/medline PY - 2002/6/12/entrez SP - 1184 EP - 90 JF - Fertility and sterility JO - Fertil. Steril. VL - 77 IS - 6 N2 - OBJECTIVE: To determine the effects of oxidative stress on the quality of oocytes and embryos, 8-hydroxy-2'-deoxyguanosine (8-OHdG) in granulosa cells was quantitatively studied during an in vitro fertilization and embryo transfer (IVF-ET) program. DESIGN: Immunocytochemical staining of 8-OHdG in granulosa cells was quantitatively estimated using a charge-coupled device camera and analyzed using the National Institute of Health Image (NIH Image) freeware on a computer . SETTING: Obstetrics and gynecology department in a university hospital. PATIENT(S): Ninety-six infertile couples undergoing IVF-ET treatment and intracytoplasmic sperm injection (IVF, n = 72; intracytoplasmic sperm injection, n = 24). INTERVENTION(S): Oocytes, granulosa cells, and follicular fluids were collected 35-36 hours after the administration of hCG. MAIN OUTCOME MEASURE(S): 8-OHdG indices were obtained for mural [8-OHdG index (m)] and cumulus [8-OHdG index (c)] granulosa cells. RESULT(S): A negative correlation between the fertilization rate and both 8-OHdG indices (c and m) was found. The rate of production of good embryos also showed a negative correlation with the 8-OHdG index (m) and the 8-OHdG index (c). Negative correlations between the 8-OHdG index (c) and E2 levels in follicular fluid were observed. Endometriosis patients showed a higher 8-OHdG index (c) than did patients with other infertility causes, such as tubal, male factor, and unknown. CONCLUSION(S): Oxidative stress in granulosa cells lowered fertilization rates and subsequently led to a decrease in the quality of embryos. The quality of oocytes for endometriosis patients was impaired by the presence of 8-OHdG. This might be one causative factor in infertility in endometriosis patients. SN - 0015-0282 UR - https://www.unboundmedicine.com/medline/citation/12057726/Eight_hydroxy_2'_deoxyguanosine_in_granulosa_cells_is_correlated_with_the_quality_of_oocytes_and_embryos_in_an_in_vitro_fertilization_embryo_transfer_program_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015028202031035 DB - PRIME DP - Unbound Medicine ER -