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Headspace solid-phase microextraction analysis of 3-alkyl-2-methoxypyrazines in wines.
J Chromatogr A. 2002 Apr 12; 953(1-2):1-6.JC

Abstract

A procedure to determine 3-alkyl-2-methoxypyrazines in wines is described. It is based on the headspace solid-phase microextraction (HS-SPME) technique after a clean-up of the sample by distillation (previously acidified to pH 0.5) to remove ethanol and other volatile compounds that can interfere in the SPME. Determination is performed by means of capillary gas chromatography using a nitrogen-phosphorus detector. The method allows quantification of 3-isobutyl-2-methoxypyrazine, 3-sec-butyl-2-methoxypyrazine and 3-isopropyl-2-methoxypyrazine at their natural concentration levels and below their sensory thresholds in Cabernet Sauvignon and Merlot wines. The method was successfully applied to experimental red wines and the evolution of their pyrazine contents during the winemaking process was monitored. Pyrazine content increased during the first maceration day but did not change significantly during alcoholic and malolactic fermentation. Final contents in wines were 12-27 ng/l of 3-isobutyl-2-methoxypyrazine and 5-10 ng/l of 3-sec-butyl-2-methoxypyrazine.

Authors+Show Affiliations

Department de Química Analítica i Química Orgànica, Facultad d'Enologia de Tarragona, Universitat Rovira i Virgili, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12058923

Citation

Sala, C, et al. "Headspace Solid-phase Microextraction Analysis of 3-alkyl-2-methoxypyrazines in Wines." Journal of Chromatography. A, vol. 953, no. 1-2, 2002, pp. 1-6.
Sala C, Mestres M, Martí MP, et al. Headspace solid-phase microextraction analysis of 3-alkyl-2-methoxypyrazines in wines. J Chromatogr A. 2002;953(1-2):1-6.
Sala, C., Mestres, M., Martí, M. P., Busto, O., & Guasch, J. (2002). Headspace solid-phase microextraction analysis of 3-alkyl-2-methoxypyrazines in wines. Journal of Chromatography. A, 953(1-2), 1-6.
Sala C, et al. Headspace Solid-phase Microextraction Analysis of 3-alkyl-2-methoxypyrazines in Wines. J Chromatogr A. 2002 Apr 12;953(1-2):1-6. PubMed PMID: 12058923.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Headspace solid-phase microextraction analysis of 3-alkyl-2-methoxypyrazines in wines. AU - Sala,C, AU - Mestres,M, AU - Martí,M P, AU - Busto,O, AU - Guasch,J, PY - 2002/6/13/pubmed PY - 2003/1/4/medline PY - 2002/6/13/entrez SP - 1 EP - 6 JF - Journal of chromatography. A JO - J Chromatogr A VL - 953 IS - 1-2 N2 - A procedure to determine 3-alkyl-2-methoxypyrazines in wines is described. It is based on the headspace solid-phase microextraction (HS-SPME) technique after a clean-up of the sample by distillation (previously acidified to pH 0.5) to remove ethanol and other volatile compounds that can interfere in the SPME. Determination is performed by means of capillary gas chromatography using a nitrogen-phosphorus detector. The method allows quantification of 3-isobutyl-2-methoxypyrazine, 3-sec-butyl-2-methoxypyrazine and 3-isopropyl-2-methoxypyrazine at their natural concentration levels and below their sensory thresholds in Cabernet Sauvignon and Merlot wines. The method was successfully applied to experimental red wines and the evolution of their pyrazine contents during the winemaking process was monitored. Pyrazine content increased during the first maceration day but did not change significantly during alcoholic and malolactic fermentation. Final contents in wines were 12-27 ng/l of 3-isobutyl-2-methoxypyrazine and 5-10 ng/l of 3-sec-butyl-2-methoxypyrazine. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/12058923/Headspace_solid_phase_microextraction_analysis_of_3_alkyl_2_methoxypyrazines_in_wines_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(02)00123-1 DB - PRIME DP - Unbound Medicine ER -