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Characterization of a novel, defense-related Arabidopsis mutant, cir1, isolated by luciferase imaging.
Mol Plant Microbe Interact. 2002 Jun; 15(6):557-66.MP

Abstract

In order to identify components of the defense signaling network engaged following attempted pathogen invasion, we generated a novel PR-1::luciferase (LUC) transgenic line that was deployed in an imaging-based screen to uncover defense-related mutants. The recessive mutant designated cir1 exhibited constitutive expression of salicylic acid (SA), jasmonic acid (JA)/ethylene, and reactive oxygen intermediate-dependent genes. Moreover, this mutation conferred resistance against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 and a virulent oomycete pathogen Peronospora parasitica Noco2. Epistasis analyses were undertaken between cir1 and mutants that disrupt the SA (nprl, nahG), JA (jar1), and ethylene (ET) (ein2) signaling pathways. While resistance against both P. syringae pv. tomato DC3000 and Peronospora parasitica Noco2 was partially reduced by npr1, resistance against both of these pathogens was lost in an nahG genetic background. Hence, cirl-mediated resistance is established via NPR1-dependent and -independent signaling pathways and SA accumulation is essential for the function of both pathways. While jar1 and ein2 reduced resistance against P. syringae pv. tomato DC3000, these mutations appeared not to impact cir1-mediated resistance against Peronospora parasitica Noco2. Thus, JA and ET sensitivity are required for cir1-mediated resistance against P. syringae pv. tomato DC3000 but not Peronospora parasitica Noco2. Therefore, the cir1 mutation may define a negative regulator of disease resistance that operates upstream of SA, JA, and ET accumulation.

Authors+Show Affiliations

Institute of Cell & Molecular Biology, University of Edinburgh, Scotland, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12059104

Citation

Murray, Shane L., et al. "Characterization of a Novel, Defense-related Arabidopsis Mutant, Cir1, Isolated By Luciferase Imaging." Molecular Plant-microbe Interactions : MPMI, vol. 15, no. 6, 2002, pp. 557-66.
Murray SL, Thomson C, Chini A, et al. Characterization of a novel, defense-related Arabidopsis mutant, cir1, isolated by luciferase imaging. Mol Plant Microbe Interact. 2002;15(6):557-66.
Murray, S. L., Thomson, C., Chini, A., Read, N. D., & Loake, G. J. (2002). Characterization of a novel, defense-related Arabidopsis mutant, cir1, isolated by luciferase imaging. Molecular Plant-microbe Interactions : MPMI, 15(6), 557-66.
Murray SL, et al. Characterization of a Novel, Defense-related Arabidopsis Mutant, Cir1, Isolated By Luciferase Imaging. Mol Plant Microbe Interact. 2002;15(6):557-66. PubMed PMID: 12059104.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of a novel, defense-related Arabidopsis mutant, cir1, isolated by luciferase imaging. AU - Murray,Shane L, AU - Thomson,Catherine, AU - Chini,Andrea, AU - Read,Nick D, AU - Loake,Gary J, PY - 2002/6/13/pubmed PY - 2003/1/8/medline PY - 2002/6/13/entrez SP - 557 EP - 66 JF - Molecular plant-microbe interactions : MPMI JO - Mol. Plant Microbe Interact. VL - 15 IS - 6 N2 - In order to identify components of the defense signaling network engaged following attempted pathogen invasion, we generated a novel PR-1::luciferase (LUC) transgenic line that was deployed in an imaging-based screen to uncover defense-related mutants. The recessive mutant designated cir1 exhibited constitutive expression of salicylic acid (SA), jasmonic acid (JA)/ethylene, and reactive oxygen intermediate-dependent genes. Moreover, this mutation conferred resistance against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 and a virulent oomycete pathogen Peronospora parasitica Noco2. Epistasis analyses were undertaken between cir1 and mutants that disrupt the SA (nprl, nahG), JA (jar1), and ethylene (ET) (ein2) signaling pathways. While resistance against both P. syringae pv. tomato DC3000 and Peronospora parasitica Noco2 was partially reduced by npr1, resistance against both of these pathogens was lost in an nahG genetic background. Hence, cirl-mediated resistance is established via NPR1-dependent and -independent signaling pathways and SA accumulation is essential for the function of both pathways. While jar1 and ein2 reduced resistance against P. syringae pv. tomato DC3000, these mutations appeared not to impact cir1-mediated resistance against Peronospora parasitica Noco2. Thus, JA and ET sensitivity are required for cir1-mediated resistance against P. syringae pv. tomato DC3000 but not Peronospora parasitica Noco2. Therefore, the cir1 mutation may define a negative regulator of disease resistance that operates upstream of SA, JA, and ET accumulation. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/12059104/Characterization_of_a_novel_defense_related_Arabidopsis_mutant_cir1_isolated_by_luciferase_imaging_ L2 - http://apsjournals.apsnet.org/doi/full/10.1094/MPMI.2002.15.6.557?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -