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Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals.
J Agric Food Chem. 2002 Jun 19; 50(13):3713-7.JA

Abstract

To express the antioxidant capacity of plant foods in a more familiar and easily understood manner (equivalent to vitamin C mg/100 g), two stable radical species, ABTS(*)(-) and DPPH(*), commonly used for antioxidant activity measurements, were employed independently to evaluate their efficacies using apple polyphenolic extracts and seven polyphenolic standards including synthetic Trolox. Their antioxidant activities were expressed as vitamin C equivalent antioxidant capacity (VCEAC) in mg/100 g apple or mg/100 mL of the reference chemical compounds in 10 and 30 min using the ABTS(*)(-) and DPPH(*) scavenging assays, respectively. The antioxidant capacity of Gala apples and seven phenolic standards, determined by both ABTS(*)(-) and DPPH(*) scavenging assays, showed a dose-response of the first-order. Fresh Gala apples had a VCEAC of 205.4 +/- 5.6 mg/100 g using the ABTS assay, and the relative VCEACs of phenolic standards were as follows: gallic acid > quercetin > epicatechin > catechin > vitamin C > rutin > chlorogenic acid > Trolox. With the DPPH radical assay, the VCEAC of fresh Gala apples was 136.0 +/- 6.6 mg/100 g, and the relative VCEACs of seven phenolic standards were, in decreasing order, as follows: gallic acid > quercetin > epicatechin > catechin > or = vitamin C > Trolox > rutin > chlorogenic acid. Because the ABTS assay can be used in both organic and aqueous solvent systems, employs a specific absorbance at a wavelength remote from the visible region, and requires a short reaction time, it is a more desirable method than the DPPH assay. Therefore, it is recommended that antioxidant capacity be expressed as vitamin C mg/100 g equivalent (VCEAC) using the ABTS assay.

Authors+Show Affiliations

Kim DO
Department of Food Science and Technology, Cornell University, Geneva, New York 14456, USA.
Lee KW
No affiliation info available
Lee HJ
No affiliation info available
Lee CY
No affiliation info available

MeSH

AntioxidantsAscorbic AcidBenzothiazolesBiphenyl CompoundsCatechinChlorogenic AcidChromansFree Radical ScavengersFree RadicalsGallic AcidKineticsMalusOxidation-ReductionPhenolsPicratesPlant ExtractsPlants, EdibleQuercetinRutinSulfonic Acids

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

12059148

Citation

Kim, Dae-Ok, et al. "Vitamin C Equivalent Antioxidant Capacity (VCEAC) of Phenolic Phytochemicals." Journal of Agricultural and Food Chemistry, vol. 50, no. 13, 2002, pp. 3713-7.
Kim DO, Lee KW, Lee HJ, et al. Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals. J Agric Food Chem. 2002;50(13):3713-7.
Kim, D. O., Lee, K. W., Lee, H. J., & Lee, C. Y. (2002). Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals. Journal of Agricultural and Food Chemistry, 50(13), 3713-7.
Kim DO, et al. Vitamin C Equivalent Antioxidant Capacity (VCEAC) of Phenolic Phytochemicals. J Agric Food Chem. 2002 Jun 19;50(13):3713-7. PubMed PMID: 12059148.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals. AU - Kim,Dae-Ok, AU - Lee,Ki Won, AU - Lee,Hyong Joo, AU - Lee,Chang Yong, PY - 2002/6/13/pubmed PY - 2002/7/26/medline PY - 2002/6/13/entrez SP - 3713 EP - 7 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 50 IS - 13 N2 - To express the antioxidant capacity of plant foods in a more familiar and easily understood manner (equivalent to vitamin C mg/100 g), two stable radical species, ABTS(*)(-) and DPPH(*), commonly used for antioxidant activity measurements, were employed independently to evaluate their efficacies using apple polyphenolic extracts and seven polyphenolic standards including synthetic Trolox. Their antioxidant activities were expressed as vitamin C equivalent antioxidant capacity (VCEAC) in mg/100 g apple or mg/100 mL of the reference chemical compounds in 10 and 30 min using the ABTS(*)(-) and DPPH(*) scavenging assays, respectively. The antioxidant capacity of Gala apples and seven phenolic standards, determined by both ABTS(*)(-) and DPPH(*) scavenging assays, showed a dose-response of the first-order. Fresh Gala apples had a VCEAC of 205.4 +/- 5.6 mg/100 g using the ABTS assay, and the relative VCEACs of phenolic standards were as follows: gallic acid > quercetin > epicatechin > catechin > vitamin C > rutin > chlorogenic acid > Trolox. With the DPPH radical assay, the VCEAC of fresh Gala apples was 136.0 +/- 6.6 mg/100 g, and the relative VCEACs of seven phenolic standards were, in decreasing order, as follows: gallic acid > quercetin > epicatechin > catechin > or = vitamin C > Trolox > rutin > chlorogenic acid. Because the ABTS assay can be used in both organic and aqueous solvent systems, employs a specific absorbance at a wavelength remote from the visible region, and requires a short reaction time, it is a more desirable method than the DPPH assay. Therefore, it is recommended that antioxidant capacity be expressed as vitamin C mg/100 g equivalent (VCEAC) using the ABTS assay. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/12059148/Vitamin_C_equivalent_antioxidant_capacity__VCEAC__of_phenolic_phytochemicals_ L2 - https://doi.org/10.1021/jf020071c DB - PRIME DP - Unbound Medicine ER -