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Diurnal rhythm of H+-peptide cotransporter in rat small intestine.

Abstract

In mammals, most physiological, biochemical, and behavioral processes show a circadian rhythm. In the present study, we examined the diurnal rhythm of the H+-peptide cotransporter (PEPT1), which transports small peptides and peptide-like drugs in the small intestine and kidney, using rats maintained in a 12-h photoperiod with free access to chow. The transport of [14C]glycylsarcosine (Gly-Sar), a typical substrate for PEPT1 by in situ intestinal loop and everted intestine, was greater in the dark phase than the light phase. PEPT1 protein and mRNA levels varied significantly, with a maximum at 2000 and minimum at 800. Similar functional and expressional diurnal variations were observed in the intestinal Na+-glucose cotransporter (SGLT1). In contrast, renal PEPT1 and SGLT1 showed little diurnal rhythmicity in protein and mRNA expression. These findings indicate that the intestinal PEPT1 undergoes diurnal regulation in its activity and expression, and this could affect the intestinal absorption of dietary protein.

Authors+Show Affiliations

Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Kyoto 606 - 8507, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12065292

Citation

Pan, Xiaoyue, et al. "Diurnal Rhythm of H+-peptide Cotransporter in Rat Small Intestine." American Journal of Physiology. Gastrointestinal and Liver Physiology, vol. 283, no. 1, 2002, pp. G57-64.
Pan X, Terada T, Irie M, et al. Diurnal rhythm of H+-peptide cotransporter in rat small intestine. Am J Physiol Gastrointest Liver Physiol. 2002;283(1):G57-64.
Pan, X., Terada, T., Irie, M., Saito, H., & Inui, K. (2002). Diurnal rhythm of H+-peptide cotransporter in rat small intestine. American Journal of Physiology. Gastrointestinal and Liver Physiology, 283(1), pp. G57-64.
Pan X, et al. Diurnal Rhythm of H+-peptide Cotransporter in Rat Small Intestine. Am J Physiol Gastrointest Liver Physiol. 2002;283(1):G57-64. PubMed PMID: 12065292.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diurnal rhythm of H+-peptide cotransporter in rat small intestine. AU - Pan,Xiaoyue, AU - Terada,Tomohiro, AU - Irie,Megumi, AU - Saito,Hideyuki, AU - Inui,Ken-Ichi, PY - 2002/6/18/pubmed PY - 2002/7/18/medline PY - 2002/6/18/entrez SP - G57 EP - 64 JF - American journal of physiology. Gastrointestinal and liver physiology JO - Am. J. Physiol. Gastrointest. Liver Physiol. VL - 283 IS - 1 N2 - In mammals, most physiological, biochemical, and behavioral processes show a circadian rhythm. In the present study, we examined the diurnal rhythm of the H+-peptide cotransporter (PEPT1), which transports small peptides and peptide-like drugs in the small intestine and kidney, using rats maintained in a 12-h photoperiod with free access to chow. The transport of [14C]glycylsarcosine (Gly-Sar), a typical substrate for PEPT1 by in situ intestinal loop and everted intestine, was greater in the dark phase than the light phase. PEPT1 protein and mRNA levels varied significantly, with a maximum at 2000 and minimum at 800. Similar functional and expressional diurnal variations were observed in the intestinal Na+-glucose cotransporter (SGLT1). In contrast, renal PEPT1 and SGLT1 showed little diurnal rhythmicity in protein and mRNA expression. These findings indicate that the intestinal PEPT1 undergoes diurnal regulation in its activity and expression, and this could affect the intestinal absorption of dietary protein. SN - 0193-1857 UR - https://www.unboundmedicine.com/medline/citation/12065292/Diurnal_rhythm_of_H+_peptide_cotransporter_in_rat_small_intestine_ L2 - http://www.physiology.org/doi/full/10.1152/ajpgi.00545.2001?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -