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Leptin increases FA oxidation in lean but not obese human skeletal muscle: evidence of peripheral leptin resistance.
Am J Physiol Endocrinol Metab 2002; 283(1):E187-92AJ

Abstract

The adipocyte-derived hormone leptin has been shown to acutely increase fatty acid (FA) oxidation and decrease esterification in resting rodent skeletal muscle. However, the effects of leptin on human skeletal muscle FA metabolism are completely unknown. In these studies, we have utilized an isolated human skeletal muscle preparation combined with the pulse-chase technique to measure FA metabolism with and without leptin in lean and obese human skeletal muscle. Under basal conditions (in the absence of leptin), muscle from the obese demonstrated significantly elevated levels of total FA uptake (+72%, P = 0.038) and enhanced rates of FA esterification into triacylglycerol (+102%, P = 0.042) compared with lean subjects. In the presence of leptin, lean muscle had elevated rates of endogenous (+103%, P = 0.01) and exogenous (+150%, P = 0.03) palmitate oxidation. When the ratio of esterification to exogenous oxidation was examined, leptin reduced this ratio (-47%, P = 0.032), demonstrating the increased partitioning of FA toward oxidation and away from storage. Contrary to these findings in lean muscle, leptin had no effect on FA metabolism in skeletal muscle of the obese. This study provides the first evidence that leptin increases FA oxidation in skeletal muscle of lean, but not obese humans, thus demonstrating the development of leptin resistance in obese human skeletal muscle.

Authors+Show Affiliations

Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1. gsteinbe@uoguelph.caNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12067860

Citation

Steinberg, Gregory R., et al. "Leptin Increases FA Oxidation in Lean but Not Obese Human Skeletal Muscle: Evidence of Peripheral Leptin Resistance." American Journal of Physiology. Endocrinology and Metabolism, vol. 283, no. 1, 2002, pp. E187-92.
Steinberg GR, Parolin ML, Heigenhauser GJ, et al. Leptin increases FA oxidation in lean but not obese human skeletal muscle: evidence of peripheral leptin resistance. Am J Physiol Endocrinol Metab. 2002;283(1):E187-92.
Steinberg, G. R., Parolin, M. L., Heigenhauser, G. J., & Dyck, D. J. (2002). Leptin increases FA oxidation in lean but not obese human skeletal muscle: evidence of peripheral leptin resistance. American Journal of Physiology. Endocrinology and Metabolism, 283(1), pp. E187-92.
Steinberg GR, et al. Leptin Increases FA Oxidation in Lean but Not Obese Human Skeletal Muscle: Evidence of Peripheral Leptin Resistance. Am J Physiol Endocrinol Metab. 2002;283(1):E187-92. PubMed PMID: 12067860.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Leptin increases FA oxidation in lean but not obese human skeletal muscle: evidence of peripheral leptin resistance. AU - Steinberg,Gregory R, AU - Parolin,Michelle L, AU - Heigenhauser,George J F, AU - Dyck,David J, PY - 2002/6/18/pubmed PY - 2002/7/12/medline PY - 2002/6/18/entrez SP - E187 EP - 92 JF - American journal of physiology. Endocrinology and metabolism JO - Am. J. Physiol. Endocrinol. Metab. VL - 283 IS - 1 N2 - The adipocyte-derived hormone leptin has been shown to acutely increase fatty acid (FA) oxidation and decrease esterification in resting rodent skeletal muscle. However, the effects of leptin on human skeletal muscle FA metabolism are completely unknown. In these studies, we have utilized an isolated human skeletal muscle preparation combined with the pulse-chase technique to measure FA metabolism with and without leptin in lean and obese human skeletal muscle. Under basal conditions (in the absence of leptin), muscle from the obese demonstrated significantly elevated levels of total FA uptake (+72%, P = 0.038) and enhanced rates of FA esterification into triacylglycerol (+102%, P = 0.042) compared with lean subjects. In the presence of leptin, lean muscle had elevated rates of endogenous (+103%, P = 0.01) and exogenous (+150%, P = 0.03) palmitate oxidation. When the ratio of esterification to exogenous oxidation was examined, leptin reduced this ratio (-47%, P = 0.032), demonstrating the increased partitioning of FA toward oxidation and away from storage. Contrary to these findings in lean muscle, leptin had no effect on FA metabolism in skeletal muscle of the obese. This study provides the first evidence that leptin increases FA oxidation in skeletal muscle of lean, but not obese humans, thus demonstrating the development of leptin resistance in obese human skeletal muscle. SN - 0193-1849 UR - https://www.unboundmedicine.com/medline/citation/12067860/Leptin_increases_FA_oxidation_in_lean_but_not_obese_human_skeletal_muscle:_evidence_of_peripheral_leptin_resistance_ L2 - http://www.physiology.org/doi/full/10.1152/ajpendo.00542.2001?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -