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SecDFyajC forms a heterotetrameric complex with YidC.
Mol Microbiol 2002; 44(5):1397-405MM

Abstract

The Escherichia coli preprotein translocase is composed of a "preprotein conducting channel" domain that consists of the peripherally bound translocation ATPase SecA and the heterotrimeric SecYEG membrane protein complex. SecD, SecF, and YajC form another heterotrimeric complex that can associate with the SecYEG complex. YidC is an essential membrane protein that plays a role in the integration of newly synthesized membrane proteins, and has been shown to co-purify with SecYEG when all translocase components are overproduced. Here, we demonstrate that under conditions that YidC co-purifies with overproduced SecDFyajC it does not co-purify with overproduced SecYEG. Moreover, this interaction of YidC with the SecDFyajC complex is also found at chromosomal protein levels of SecD, SecF and YajC. Closer examination of the SecDFyajC-YidC complex showed that YidC binds to SecD and SecF, whereas YajC interacts only with SecF. As SecF and YajC have previously been shown to interact with SecY, we propose that these two proteins link the heterotetrameric SecDFyajC-YidC complex to the SecYEG complex.

Authors+Show Affiliations

Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands. n.nouwen@biol.rug.nlNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12068816

Citation

Nouwen, Nico, and Arnold J M. Driessen. "SecDFyajC Forms a Heterotetrameric Complex With YidC." Molecular Microbiology, vol. 44, no. 5, 2002, pp. 1397-405.
Nouwen N, Driessen AJ. SecDFyajC forms a heterotetrameric complex with YidC. Mol Microbiol. 2002;44(5):1397-405.
Nouwen, N., & Driessen, A. J. (2002). SecDFyajC forms a heterotetrameric complex with YidC. Molecular Microbiology, 44(5), pp. 1397-405.
Nouwen N, Driessen AJ. SecDFyajC Forms a Heterotetrameric Complex With YidC. Mol Microbiol. 2002;44(5):1397-405. PubMed PMID: 12068816.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SecDFyajC forms a heterotetrameric complex with YidC. AU - Nouwen,Nico, AU - Driessen,Arnold J M, PY - 2002/6/19/pubmed PY - 2002/11/28/medline PY - 2002/6/19/entrez SP - 1397 EP - 405 JF - Molecular microbiology JO - Mol. Microbiol. VL - 44 IS - 5 N2 - The Escherichia coli preprotein translocase is composed of a "preprotein conducting channel" domain that consists of the peripherally bound translocation ATPase SecA and the heterotrimeric SecYEG membrane protein complex. SecD, SecF, and YajC form another heterotrimeric complex that can associate with the SecYEG complex. YidC is an essential membrane protein that plays a role in the integration of newly synthesized membrane proteins, and has been shown to co-purify with SecYEG when all translocase components are overproduced. Here, we demonstrate that under conditions that YidC co-purifies with overproduced SecDFyajC it does not co-purify with overproduced SecYEG. Moreover, this interaction of YidC with the SecDFyajC complex is also found at chromosomal protein levels of SecD, SecF and YajC. Closer examination of the SecDFyajC-YidC complex showed that YidC binds to SecD and SecF, whereas YajC interacts only with SecF. As SecF and YajC have previously been shown to interact with SecY, we propose that these two proteins link the heterotetrameric SecDFyajC-YidC complex to the SecYEG complex. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/12068816/SecDFyajC_forms_a_heterotetrameric_complex_with_YidC_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=2002&volume=44&issue=5&spage=1397 DB - PRIME DP - Unbound Medicine ER -