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Diheteroarylethenes as thermally stable photoswitchable acceptors in photochromic fluorescence resonance energy transfer (pcFRET).
J Am Chem Soc 2002; 124(25):7481-9JA

Abstract

We have employed diheteroarylethenes as acceptors for photochromic FRET (pcFRET), a technique introduced for the quantitative determination of fluorescence resonance energy transfer (FRET). In pcFRET, the fluorescent emission of the donor is modulated by cyclical transformations of a photochromic acceptor. Light induces a reversible change in the structure and, concomitantly, in the absorption properties of the acceptor. Only the closed forms of the selected diheteroarylethenes 2a and 2b have an absorption band overlapping the emission band of the donor, 1. The corresponding variation in the overlap integral (and thus critical transfer distance R(o)) between the two states provides the means for reversibly switching the process of FRET on and off, allowing direct and repeated evaluation of the relative changes in the donor fluorescence quantum yield. The diheteroarylethenes demonstrate excellent stability in aqueous media, an absence of thermal back reactions, and negligible fatigue. The equilibration of these systems after exposure to near-UV or visible light follows simple monoexponential kinetics. We developed a general conceptual scheme for such coupled photochromic-FRET reactions, allowing quantitative interpretations of the photostationary and kinetic data, from which the quantum yields for the cyclization and cycloreversion reactions of the photochromic acceptor were calculated.

Authors+Show Affiliations

Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, PROPLAME-CONICET, Ciudad Universitaria-Pabellón II, 1428 Buenos Aires, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12071757

Citation

Giordano, Luciana, et al. "Diheteroarylethenes as Thermally Stable Photoswitchable Acceptors in Photochromic Fluorescence Resonance Energy Transfer (pcFRET)." Journal of the American Chemical Society, vol. 124, no. 25, 2002, pp. 7481-9.
Giordano L, Jovin TM, Irie M, et al. Diheteroarylethenes as thermally stable photoswitchable acceptors in photochromic fluorescence resonance energy transfer (pcFRET). J Am Chem Soc. 2002;124(25):7481-9.
Giordano, L., Jovin, T. M., Irie, M., & Jares-Erijman, E. A. (2002). Diheteroarylethenes as thermally stable photoswitchable acceptors in photochromic fluorescence resonance energy transfer (pcFRET). Journal of the American Chemical Society, 124(25), pp. 7481-9.
Giordano L, et al. Diheteroarylethenes as Thermally Stable Photoswitchable Acceptors in Photochromic Fluorescence Resonance Energy Transfer (pcFRET). J Am Chem Soc. 2002 Jun 26;124(25):7481-9. PubMed PMID: 12071757.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diheteroarylethenes as thermally stable photoswitchable acceptors in photochromic fluorescence resonance energy transfer (pcFRET). AU - Giordano,Luciana, AU - Jovin,Thomas M, AU - Irie,Masahiro, AU - Jares-Erijman,Elizabeth A, PY - 2002/6/20/pubmed PY - 2002/6/20/medline PY - 2002/6/20/entrez SP - 7481 EP - 9 JF - Journal of the American Chemical Society JO - J. Am. Chem. Soc. VL - 124 IS - 25 N2 - We have employed diheteroarylethenes as acceptors for photochromic FRET (pcFRET), a technique introduced for the quantitative determination of fluorescence resonance energy transfer (FRET). In pcFRET, the fluorescent emission of the donor is modulated by cyclical transformations of a photochromic acceptor. Light induces a reversible change in the structure and, concomitantly, in the absorption properties of the acceptor. Only the closed forms of the selected diheteroarylethenes 2a and 2b have an absorption band overlapping the emission band of the donor, 1. The corresponding variation in the overlap integral (and thus critical transfer distance R(o)) between the two states provides the means for reversibly switching the process of FRET on and off, allowing direct and repeated evaluation of the relative changes in the donor fluorescence quantum yield. The diheteroarylethenes demonstrate excellent stability in aqueous media, an absence of thermal back reactions, and negligible fatigue. The equilibration of these systems after exposure to near-UV or visible light follows simple monoexponential kinetics. We developed a general conceptual scheme for such coupled photochromic-FRET reactions, allowing quantitative interpretations of the photostationary and kinetic data, from which the quantum yields for the cyclization and cycloreversion reactions of the photochromic acceptor were calculated. SN - 0002-7863 UR - https://www.unboundmedicine.com/medline/citation/12071757/Diheteroarylethenes_as_thermally_stable_photoswitchable_acceptors_in_photochromic_fluorescence_resonance_energy_transfer__pcFRET__ L2 - https://dx.doi.org/10.1021/ja016969k DB - PRIME DP - Unbound Medicine ER -