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Towards a genus-wide reference linkage map for Eucalyptus based exclusively on highly informative microsatellite markers.
Mol Genet Genomics. 2002 May; 267(3):338-47.MG

Abstract

A novel set of 50 highly polymorphic microsatellite markers were developed and mapped on existing RAPD framework maps of Eucalyptus grandis and E. urophylla. Together with the twenty previously developed microsatellite markers, these were used to align the existing maps for the two most commercially important Eucalyptus species in the tropics. Sixty-three microsatellite markers were placed on the E. grandis map in 11 linkage groups, and 53 on the E. urophylla map distributed in 10 linkage groups. Approximately 66% of the microsatellite markers segregated in a fully informative fashion, allowing the establishment of colinear syntenic linkage groups between the two maps. The 50 new microsatellite markers were highly informative, with an average of 14 alleles per locus, and average expected heterozygosity between 0.82 and 0.87. Furthermore, within the subgenus Symphyomyrtus, to which the vast majority of commercially important Eucalyptus species belong, these markers display on average 90% transportability. This set of 70 mapped microsatellite markers represents a significant step toward the development of a genus-wide reference linkage map for Eucalyptus. These highly multiallelic and transportable markers constitute a powerful tool for QTL discovery and validation, and can be used in directed searches for QTL allele variation across Eucalyptus pedigrees.

Authors+Show Affiliations

Deparetaments de Biologia Celular, Universidade de Brasilia, Brasilia D.F., Brazil. rosanavb@cnpaf.embrapa.brNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12073036

Citation

Brondani, Rosana P V., et al. "Towards a Genus-wide Reference Linkage Map for Eucalyptus Based Exclusively On Highly Informative Microsatellite Markers." Molecular Genetics and Genomics : MGG, vol. 267, no. 3, 2002, pp. 338-47.
Brondani RP, Brondani C, Grattapaglia D. Towards a genus-wide reference linkage map for Eucalyptus based exclusively on highly informative microsatellite markers. Mol Genet Genomics. 2002;267(3):338-47.
Brondani, R. P., Brondani, C., & Grattapaglia, D. (2002). Towards a genus-wide reference linkage map for Eucalyptus based exclusively on highly informative microsatellite markers. Molecular Genetics and Genomics : MGG, 267(3), 338-47.
Brondani RP, Brondani C, Grattapaglia D. Towards a Genus-wide Reference Linkage Map for Eucalyptus Based Exclusively On Highly Informative Microsatellite Markers. Mol Genet Genomics. 2002;267(3):338-47. PubMed PMID: 12073036.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Towards a genus-wide reference linkage map for Eucalyptus based exclusively on highly informative microsatellite markers. AU - Brondani,Rosana P V, AU - Brondani,C, AU - Grattapaglia,D, Y1 - 2002/04/05/ PY - 2001/10/25/received PY - 2002/02/20/accepted PY - 2002/6/20/pubmed PY - 2002/7/23/medline PY - 2002/6/20/entrez SP - 338 EP - 47 JF - Molecular genetics and genomics : MGG JO - Mol. Genet. Genomics VL - 267 IS - 3 N2 - A novel set of 50 highly polymorphic microsatellite markers were developed and mapped on existing RAPD framework maps of Eucalyptus grandis and E. urophylla. Together with the twenty previously developed microsatellite markers, these were used to align the existing maps for the two most commercially important Eucalyptus species in the tropics. Sixty-three microsatellite markers were placed on the E. grandis map in 11 linkage groups, and 53 on the E. urophylla map distributed in 10 linkage groups. Approximately 66% of the microsatellite markers segregated in a fully informative fashion, allowing the establishment of colinear syntenic linkage groups between the two maps. The 50 new microsatellite markers were highly informative, with an average of 14 alleles per locus, and average expected heterozygosity between 0.82 and 0.87. Furthermore, within the subgenus Symphyomyrtus, to which the vast majority of commercially important Eucalyptus species belong, these markers display on average 90% transportability. This set of 70 mapped microsatellite markers represents a significant step toward the development of a genus-wide reference linkage map for Eucalyptus. These highly multiallelic and transportable markers constitute a powerful tool for QTL discovery and validation, and can be used in directed searches for QTL allele variation across Eucalyptus pedigrees. SN - 1617-4615 UR - https://www.unboundmedicine.com/medline/citation/12073036/Towards_a_genus_wide_reference_linkage_map_for_Eucalyptus_based_exclusively_on_highly_informative_microsatellite_markers_ L2 - https://dx.doi.org/10.1007/s00438-002-0665-6 DB - PRIME DP - Unbound Medicine ER -