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Characterization of Cu, Zn-superoxide dismutase-deficient mutant of fission yeast Schizosaccharomyces pombe.
Curr Genet. 2002 May; 41(2):82-8.CG

Abstract

A Cu, Zn-superoxide dismutase gene (sod1+) deletion mutant of fission yeast Schizosaccharomyces pombe was constructed and its properties were investigated. Superoxide dismutase activity was not detected in the mutant on activity staining of polyacrylamide gels. The mutant showed cysteine or methionine and lysine auxotrophy, slow growth and sensitivity to menadione. While expression of the apt1+ gene, induction of which depends on the Pap1 transcription factor, was induced at the same concentration of menadione in both the wild-type cell and the sod1 mutant, expression of the gpx1+ gene, induction of which depends on the Atf1 transcription factor, was induced at a lower concentration of menadione in the mutant compared with the wild-type control. Expression of the sod1+ gene was induced by oxidative stress and no induction was observed in pap1, prr1 and spc1 mutants.

Authors+Show Affiliations

Department of Genetics, Institute for Developmental Research, Aichi Human Service Center, Kagiya-cho, Kasugai, Aichi 480-0392, Japan. normutoh@inst-hsc.pref.aichi.jpNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12073089

Citation

Mutoh, Norihiro, et al. "Characterization of Cu, Zn-superoxide Dismutase-deficient Mutant of Fission Yeast Schizosaccharomyces Pombe." Current Genetics, vol. 41, no. 2, 2002, pp. 82-8.
Mutoh N, Nakagawa CW, Yamada K. Characterization of Cu, Zn-superoxide dismutase-deficient mutant of fission yeast Schizosaccharomyces pombe. Curr Genet. 2002;41(2):82-8.
Mutoh, N., Nakagawa, C. W., & Yamada, K. (2002). Characterization of Cu, Zn-superoxide dismutase-deficient mutant of fission yeast Schizosaccharomyces pombe. Current Genetics, 41(2), 82-8.
Mutoh N, Nakagawa CW, Yamada K. Characterization of Cu, Zn-superoxide Dismutase-deficient Mutant of Fission Yeast Schizosaccharomyces Pombe. Curr Genet. 2002;41(2):82-8. PubMed PMID: 12073089.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of Cu, Zn-superoxide dismutase-deficient mutant of fission yeast Schizosaccharomyces pombe. AU - Mutoh,Norihiro, AU - Nakagawa,Chiaki W, AU - Yamada,Kenichiro, Y1 - 2002/04/19/ PY - 2001/09/28/received PY - 2002/03/04/accepted PY - 2002/6/20/pubmed PY - 2002/12/12/medline PY - 2002/6/20/entrez SP - 82 EP - 8 JF - Current genetics JO - Curr Genet VL - 41 IS - 2 N2 - A Cu, Zn-superoxide dismutase gene (sod1+) deletion mutant of fission yeast Schizosaccharomyces pombe was constructed and its properties were investigated. Superoxide dismutase activity was not detected in the mutant on activity staining of polyacrylamide gels. The mutant showed cysteine or methionine and lysine auxotrophy, slow growth and sensitivity to menadione. While expression of the apt1+ gene, induction of which depends on the Pap1 transcription factor, was induced at the same concentration of menadione in both the wild-type cell and the sod1 mutant, expression of the gpx1+ gene, induction of which depends on the Atf1 transcription factor, was induced at a lower concentration of menadione in the mutant compared with the wild-type control. Expression of the sod1+ gene was induced by oxidative stress and no induction was observed in pap1, prr1 and spc1 mutants. SN - 0172-8083 UR - https://www.unboundmedicine.com/medline/citation/12073089/Characterization_of_Cu_Zn_superoxide_dismutase_deficient_mutant_of_fission_yeast_Schizosaccharomyces_pombe_ L2 - https://doi.org/10.1007/s00294-002-0288-9 DB - PRIME DP - Unbound Medicine ER -