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The evaluation of autoantibodies against oxidatively modified low-density lipoprotein (LDL), susceptibility of LDL to oxidation, serum lipids and lipid hydroperoxide levels, total antioxidant status, antioxidant enzyme activities, and endothelial dysfunction in patients with Behçet's disease.

Abstract

OBJECTIVES

Behçet's disease is a multisystem disorder characterized by a chronic inflammation including acute attacks and remission periods. Decreased enzyme activity of the antioxidant system and increased levels of free radicals may have important roles in the damage of tissues observed in the disease period. In addition, the atherogenic tendency of serum lipid, lipoproteins, lipid peroxidation levels and endothelial dysfunction accompany the above mentioned findings. As a consequence of these events, different degrees of low density lipoprotein (LDL) oxidation occur in vivo, and then autoantibodies against oxidized-LDL(AuAb-oxLDL) are produced.

DESIGN AND METHODS

Lipids, lipoproteins, lipid hydroperoxide, AuAb-oxLDL, total antioxidant status (TAS), serum-soluble intercellular adhesion molecule-1 (sICAM-1), plasminogen activator inhibitor 1 (PAI-1) levels in serum, the activities of antioxidant enzymes including glutathione peroxidase (GSH-Px), glutathione reductase (GR), superoxide dismutase (SOD), and catalase (CAT) in erythrocytes and plasma, were determined in 25 patients with Behçet's disease and in 25 healthy volunteers. Also, susceptibility to copper-induced in vitro oxidation of LDL by using lag time, a measure of resistance to oxidation, oxidation rate and extent of oxidation, a measure of diene production in both groups, was studied.

RESULTS

It was observed that lipid hydroperoxide and AuAb-oxLDL levels in patients with Behçet's disease were significantly higher, but erythrocyte SOD, CAT, plasma GSH-Px activities, and TAS were significantly lower than those in healthy subjects. Susceptibility of LDL to oxidation in the patients was found to be increased. Total cholesterol, LDL-C and apo B levels and acute phase reactants were significantly higher, but HDL-C and apo AI levels were significantly lower, in patients when compared to healthy subjects. The levels of AuAb-oxLDL in patients were found to correlate with TAS, total cholesterol, LDL-C, lipid hydroperoxide and erythrocyte SOD activities (r = -0.62, p < 0.01; r = 0.64, p < 0.01; r = 0.55, p < 0.01; r = 0.81, p < 0.01; r = -0.63, p < 0.01, respectively). In addition, lipid hydroperoxide levels were found to correlate with total cholesterol, LDL-C and erythrocyte SOD activities (r = 0.45, p < 0.05; r = 0.45, p < 0.05; r = -0.46, p < 0.05, respectively). PAI-1 and sICAM-1 were found to be increased in the patients and correlated with AuAb-oxLDL and lipid hydroperoxide levels (r = 0.56, p < 0.01; r = 0.67, p < 0.01 and r = 0.59, p < 0.01; r = 0.61, p < 0.01, respectively).

CONCLUSIONS

It was concluded that the observed increase of lipid, lipoproteins, lipid hydroperoxide, susceptibility of LDL to oxidation, autoantibodies against ox-LDL levels and decrease of antioxidant enzyme activities and total antioxidant status and increased secretion of endothelial derivated peptides including sICAM and PAI-1, and their interactions may indicate that there is a tendency to atherothrombotic events in patients with Behçet's disease.

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  • Authors+Show Affiliations

    ,

    Department of Biochemistry, Faculty of Medicine, Karadeniz Technical University, 61080, Trabzon, Turkey. aorem64@yahoo.com

    , , , ,

    Source

    Clinical biochemistry 35:3 2002 May pg 217-24

    MeSH

    Adult
    Antioxidants
    Autoantibodies
    Behcet Syndrome
    Catalase
    Endothelium
    Female
    Glutathione Peroxidase
    Glutathione Reductase
    Humans
    Lipid Peroxides
    Lipids
    Lipoproteins, LDL
    Male
    Middle Aged
    Oxidation-Reduction
    Superoxide Dismutase

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    12074830

    Citation

    Orem, Asim, et al. "The Evaluation of Autoantibodies Against Oxidatively Modified Low-density Lipoprotein (LDL), Susceptibility of LDL to Oxidation, Serum Lipids and Lipid Hydroperoxide Levels, Total Antioxidant Status, Antioxidant Enzyme Activities, and Endothelial Dysfunction in Patients With Behçet's Disease." Clinical Biochemistry, vol. 35, no. 3, 2002, pp. 217-24.
    Orem A, Yandi YE, Vanizor B, et al. The evaluation of autoantibodies against oxidatively modified low-density lipoprotein (LDL), susceptibility of LDL to oxidation, serum lipids and lipid hydroperoxide levels, total antioxidant status, antioxidant enzyme activities, and endothelial dysfunction in patients with Behçet's disease. Clin Biochem. 2002;35(3):217-24.
    Orem, A., Yandi, Y. E., Vanizor, B., Cimşit, G., Uydu, H. A., & Malkoç, M. (2002). The evaluation of autoantibodies against oxidatively modified low-density lipoprotein (LDL), susceptibility of LDL to oxidation, serum lipids and lipid hydroperoxide levels, total antioxidant status, antioxidant enzyme activities, and endothelial dysfunction in patients with Behçet's disease. Clinical Biochemistry, 35(3), pp. 217-24.
    Orem A, et al. The Evaluation of Autoantibodies Against Oxidatively Modified Low-density Lipoprotein (LDL), Susceptibility of LDL to Oxidation, Serum Lipids and Lipid Hydroperoxide Levels, Total Antioxidant Status, Antioxidant Enzyme Activities, and Endothelial Dysfunction in Patients With Behçet's Disease. Clin Biochem. 2002;35(3):217-24. PubMed PMID: 12074830.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - The evaluation of autoantibodies against oxidatively modified low-density lipoprotein (LDL), susceptibility of LDL to oxidation, serum lipids and lipid hydroperoxide levels, total antioxidant status, antioxidant enzyme activities, and endothelial dysfunction in patients with Behçet's disease. AU - Orem,Asim, AU - Yandi,Y Emre, AU - Vanizor,Birgül, AU - Cimşit,Gülseren, AU - Uydu,Hüseyin Avni, AU - Malkoç,Meltem, PY - 2002/6/21/pubmed PY - 2002/12/5/medline PY - 2002/6/21/entrez SP - 217 EP - 24 JF - Clinical biochemistry JO - Clin. Biochem. VL - 35 IS - 3 N2 - OBJECTIVES: Behçet's disease is a multisystem disorder characterized by a chronic inflammation including acute attacks and remission periods. Decreased enzyme activity of the antioxidant system and increased levels of free radicals may have important roles in the damage of tissues observed in the disease period. In addition, the atherogenic tendency of serum lipid, lipoproteins, lipid peroxidation levels and endothelial dysfunction accompany the above mentioned findings. As a consequence of these events, different degrees of low density lipoprotein (LDL) oxidation occur in vivo, and then autoantibodies against oxidized-LDL(AuAb-oxLDL) are produced. DESIGN AND METHODS: Lipids, lipoproteins, lipid hydroperoxide, AuAb-oxLDL, total antioxidant status (TAS), serum-soluble intercellular adhesion molecule-1 (sICAM-1), plasminogen activator inhibitor 1 (PAI-1) levels in serum, the activities of antioxidant enzymes including glutathione peroxidase (GSH-Px), glutathione reductase (GR), superoxide dismutase (SOD), and catalase (CAT) in erythrocytes and plasma, were determined in 25 patients with Behçet's disease and in 25 healthy volunteers. Also, susceptibility to copper-induced in vitro oxidation of LDL by using lag time, a measure of resistance to oxidation, oxidation rate and extent of oxidation, a measure of diene production in both groups, was studied. RESULTS: It was observed that lipid hydroperoxide and AuAb-oxLDL levels in patients with Behçet's disease were significantly higher, but erythrocyte SOD, CAT, plasma GSH-Px activities, and TAS were significantly lower than those in healthy subjects. Susceptibility of LDL to oxidation in the patients was found to be increased. Total cholesterol, LDL-C and apo B levels and acute phase reactants were significantly higher, but HDL-C and apo AI levels were significantly lower, in patients when compared to healthy subjects. The levels of AuAb-oxLDL in patients were found to correlate with TAS, total cholesterol, LDL-C, lipid hydroperoxide and erythrocyte SOD activities (r = -0.62, p < 0.01; r = 0.64, p < 0.01; r = 0.55, p < 0.01; r = 0.81, p < 0.01; r = -0.63, p < 0.01, respectively). In addition, lipid hydroperoxide levels were found to correlate with total cholesterol, LDL-C and erythrocyte SOD activities (r = 0.45, p < 0.05; r = 0.45, p < 0.05; r = -0.46, p < 0.05, respectively). PAI-1 and sICAM-1 were found to be increased in the patients and correlated with AuAb-oxLDL and lipid hydroperoxide levels (r = 0.56, p < 0.01; r = 0.67, p < 0.01 and r = 0.59, p < 0.01; r = 0.61, p < 0.01, respectively). CONCLUSIONS: It was concluded that the observed increase of lipid, lipoproteins, lipid hydroperoxide, susceptibility of LDL to oxidation, autoantibodies against ox-LDL levels and decrease of antioxidant enzyme activities and total antioxidant status and increased secretion of endothelial derivated peptides including sICAM and PAI-1, and their interactions may indicate that there is a tendency to atherothrombotic events in patients with Behçet's disease. SN - 0009-9120 UR - https://www.unboundmedicine.com/medline/citation/12074830/The_evaluation_of_autoantibodies_against_oxidatively_modified_low_density_lipoprotein__LDL__susceptibility_of_LDL_to_oxidation_serum_lipids_and_lipid_hydroperoxide_levels_total_antioxidant_status_antioxidant_enzyme_activities_and_endothelial_dysfunction_in_patients_with_Behçet's_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009912002002904 DB - PRIME DP - Unbound Medicine ER -