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Polyphenols and red wine as peroxynitrite scavengers: a chemiluminescent assay.
Ann N Y Acad Sci. 2002 May; 957:271-3.AN

Abstract

A novel chemiluminescent assay for evaluating peroxynitrite (ONOO(-))-scavenging capacity was developed. The experimental protocol ensures sensitivity and reproducibility of measurements. The addition of 0-500 microM ONOO(-) to rat liver homogenate generated a luminous signal that was analyzed by chemiluminescence in a LKB Wallac liquid scintillation counter. The obtained optimal conditions were: 1-2 mg/mL of homogenate protein in 120 mM KCl, 30 mM phosphate buffer (pH 7.4), and 220 microM ONOO(-) at 30 degrees C. As polyphenols we used (+)-catechin, (-)-epicatechin, and myricetin. The most efficient of the compounds tested was myricetin with an IC(50) of 20 microM. The effectiveness of this method was verified by evaluating the antioxidant ability of three red wine samples to decrease peroxynitrite-initiated chemiluminescence. The ONOO(-)-scavenging activity of wines measured by this assay was related to the phenolic level of the samples. The quickness and reliability of this assay makes it particularly suitable for a large-scale screening of watery food extracts.

Authors+Show Affiliations

Physical Chemistry - PRALIB, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina. salvarez@ffyb.uba.arNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12074979

Citation

Alvarez, Silvia, et al. "Polyphenols and Red Wine as Peroxynitrite Scavengers: a Chemiluminescent Assay." Annals of the New York Academy of Sciences, vol. 957, 2002, pp. 271-3.
Alvarez S, Zaobornyj T, Actis-Goretta L, et al. Polyphenols and red wine as peroxynitrite scavengers: a chemiluminescent assay. Ann N Y Acad Sci. 2002;957:271-3.
Alvarez, S., Zaobornyj, T., Actis-Goretta, L., Fraga, C. G., & Boveris, A. (2002). Polyphenols and red wine as peroxynitrite scavengers: a chemiluminescent assay. Annals of the New York Academy of Sciences, 957, 271-3.
Alvarez S, et al. Polyphenols and Red Wine as Peroxynitrite Scavengers: a Chemiluminescent Assay. Ann N Y Acad Sci. 2002;957:271-3. PubMed PMID: 12074979.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polyphenols and red wine as peroxynitrite scavengers: a chemiluminescent assay. AU - Alvarez,Silvia, AU - Zaobornyj,Tamara, AU - Actis-Goretta,Lucas, AU - Fraga,César G, AU - Boveris,Alberto, PY - 2002/6/21/pubmed PY - 2002/8/1/medline PY - 2002/6/21/entrez SP - 271 EP - 3 JF - Annals of the New York Academy of Sciences JO - Ann. N. Y. Acad. Sci. VL - 957 N2 - A novel chemiluminescent assay for evaluating peroxynitrite (ONOO(-))-scavenging capacity was developed. The experimental protocol ensures sensitivity and reproducibility of measurements. The addition of 0-500 microM ONOO(-) to rat liver homogenate generated a luminous signal that was analyzed by chemiluminescence in a LKB Wallac liquid scintillation counter. The obtained optimal conditions were: 1-2 mg/mL of homogenate protein in 120 mM KCl, 30 mM phosphate buffer (pH 7.4), and 220 microM ONOO(-) at 30 degrees C. As polyphenols we used (+)-catechin, (-)-epicatechin, and myricetin. The most efficient of the compounds tested was myricetin with an IC(50) of 20 microM. The effectiveness of this method was verified by evaluating the antioxidant ability of three red wine samples to decrease peroxynitrite-initiated chemiluminescence. The ONOO(-)-scavenging activity of wines measured by this assay was related to the phenolic level of the samples. The quickness and reliability of this assay makes it particularly suitable for a large-scale screening of watery food extracts. SN - 0077-8923 UR - https://www.unboundmedicine.com/medline/citation/12074979/Polyphenols_and_red_wine_as_peroxynitrite_scavengers:_a_chemiluminescent_assay_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0077-8923&date=2002&volume=957&spage=271 DB - PRIME DP - Unbound Medicine ER -