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Characterisation of multiple Caribbean ciguatoxins and congeners in individual specimens of horse-eye jack (Caranx latus) by high-performance liquid chromatography/mass spectrometry.
Toxicon. 2002 Jul; 40(7):929-39.T

Abstract

We studied the variation in toxin profiles of purified extracts of 10 individual specimens and two pools of ciguateric Caranx latus. High-performance liquid chromatography/mass spectrometry (HPLC/MS) identified in all individual samples at least seven Caribbean ciguatoxins (C-CTXs) comprising C-CTX-1 and its epimer C-CTX-2 ([M+H](+) m/z 1141.58), and five new C-CTX congeners with pseudo-molecular ions at m/z 1141.58, 1143.60, 1157.57, 1159.58, and 1127.57. In some samples, additional C-CTX isomers were detected with [M+H](+) ions at m/z 1141.58 (two), 1143.60 (one) and 1157.57 (two). The two low-toxic pools contained only four to six ciguatoxins. The comparison in relative proportions of four different mass classes ([M+H](+) at m/z 1141, 1143, 1157 and 1127) showed that the group at m/z 1157 increased (2-20%) with flesh toxicity. More than 80% of group m/z 1141 comprised C-CTX-1, C-CTX-2 and their isomer C-CTX-1a whose level in this group correlated with fish toxicity. Contrary to low-toxic fishes, high-risk specimens had C-CTX-1 levels <50% and were subjected to large losses of activity on purification indicating that unstable ciguatoxins were present. A possible conversion of C-CTX-1 into C-CTX-1a was identified when flesh was cooked, without changes in toxicity. In conclusion, HPLC/MS characterised 12 C-CTXs accumulated by C. latus at variable levels.

Authors+Show Affiliations

Laboratoire de Microbiologie Alimentaire, Universite de Caen, Esplanade de la Paix, 14032 Caen cedex, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12076647

Citation

Pottier, Ivannah, et al. "Characterisation of Multiple Caribbean Ciguatoxins and Congeners in Individual Specimens of Horse-eye Jack (Caranx Latus) By High-performance Liquid Chromatography/mass Spectrometry." Toxicon : Official Journal of the International Society On Toxinology, vol. 40, no. 7, 2002, pp. 929-39.
Pottier I, Vernoux JP, Jones A, et al. Characterisation of multiple Caribbean ciguatoxins and congeners in individual specimens of horse-eye jack (Caranx latus) by high-performance liquid chromatography/mass spectrometry. Toxicon. 2002;40(7):929-39.
Pottier, I., Vernoux, J. P., Jones, A., & Lewis, R. J. (2002). Characterisation of multiple Caribbean ciguatoxins and congeners in individual specimens of horse-eye jack (Caranx latus) by high-performance liquid chromatography/mass spectrometry. Toxicon : Official Journal of the International Society On Toxinology, 40(7), 929-39.
Pottier I, et al. Characterisation of Multiple Caribbean Ciguatoxins and Congeners in Individual Specimens of Horse-eye Jack (Caranx Latus) By High-performance Liquid Chromatography/mass Spectrometry. Toxicon. 2002;40(7):929-39. PubMed PMID: 12076647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterisation of multiple Caribbean ciguatoxins and congeners in individual specimens of horse-eye jack (Caranx latus) by high-performance liquid chromatography/mass spectrometry. AU - Pottier,Ivannah, AU - Vernoux,Jean-Paul, AU - Jones,Alun, AU - Lewis,Richard J, PY - 2002/6/22/pubmed PY - 2003/2/8/medline PY - 2002/6/22/entrez SP - 929 EP - 39 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 40 IS - 7 N2 - We studied the variation in toxin profiles of purified extracts of 10 individual specimens and two pools of ciguateric Caranx latus. High-performance liquid chromatography/mass spectrometry (HPLC/MS) identified in all individual samples at least seven Caribbean ciguatoxins (C-CTXs) comprising C-CTX-1 and its epimer C-CTX-2 ([M+H](+) m/z 1141.58), and five new C-CTX congeners with pseudo-molecular ions at m/z 1141.58, 1143.60, 1157.57, 1159.58, and 1127.57. In some samples, additional C-CTX isomers were detected with [M+H](+) ions at m/z 1141.58 (two), 1143.60 (one) and 1157.57 (two). The two low-toxic pools contained only four to six ciguatoxins. The comparison in relative proportions of four different mass classes ([M+H](+) at m/z 1141, 1143, 1157 and 1127) showed that the group at m/z 1157 increased (2-20%) with flesh toxicity. More than 80% of group m/z 1141 comprised C-CTX-1, C-CTX-2 and their isomer C-CTX-1a whose level in this group correlated with fish toxicity. Contrary to low-toxic fishes, high-risk specimens had C-CTX-1 levels <50% and were subjected to large losses of activity on purification indicating that unstable ciguatoxins were present. A possible conversion of C-CTX-1 into C-CTX-1a was identified when flesh was cooked, without changes in toxicity. In conclusion, HPLC/MS characterised 12 C-CTXs accumulated by C. latus at variable levels. SN - 0041-0101 UR - https://www.unboundmedicine.com/medline/citation/12076647/Characterisation_of_multiple_Caribbean_ciguatoxins_and_congeners_in_individual_specimens_of_horse_eye_jack__Caranx_latus__by_high_performance_liquid_chromatography/mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041010102000880 DB - PRIME DP - Unbound Medicine ER -