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Mechanism of action of the Escherichia coli phage shock protein PspA in repression of the AAA family transcription factor PspF.
J Mol Biol. 2002 Jun 28; 320(1):23-37.JM

Abstract

The PspA protein, a negative regulator of the Escherichia coli phage shock psp operon, is produced when virulence factors are exported through secretins in many Gram-negative pathogenic bacteria and its homologue in plants, VIPP1, plays a critical role in thylakoid biogenesis, essential for photosynthesis. Activation of transcription by the enhancer-dependent bacterial sigma(54) containing RNA polymerase occurs through ATP hydrolysis-driven protein conformational changes enabled by activator proteins that belong to the large AAA(+) mechanochemical protein family. We show that PspA directly and specifically acts upon and binds to the AAA(+) domain of the PspF transcription activator. Interactions involving PspF and nucleotide are changed by the action of PspA. These changes and the complexes that form between PspF and PspA can explain how PspA exerts its negative effects upon transcription activated by PspF, and are of significance when considering how activities of other AAA(+) proteins might be controlled.

Authors+Show Affiliations

Department of Biological Sciences, Imperial College of Science Technology and Medicine, Biomedical Sciences Building, Imperial College Road, London SW7 2AZ, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12079332

Citation

Elderkin, Sarah, et al. "Mechanism of Action of the Escherichia Coli Phage Shock Protein PspA in Repression of the AAA Family Transcription Factor PspF." Journal of Molecular Biology, vol. 320, no. 1, 2002, pp. 23-37.
Elderkin S, Jones S, Schumacher J, et al. Mechanism of action of the Escherichia coli phage shock protein PspA in repression of the AAA family transcription factor PspF. J Mol Biol. 2002;320(1):23-37.
Elderkin, S., Jones, S., Schumacher, J., Studholme, D., & Buck, M. (2002). Mechanism of action of the Escherichia coli phage shock protein PspA in repression of the AAA family transcription factor PspF. Journal of Molecular Biology, 320(1), 23-37.
Elderkin S, et al. Mechanism of Action of the Escherichia Coli Phage Shock Protein PspA in Repression of the AAA Family Transcription Factor PspF. J Mol Biol. 2002 Jun 28;320(1):23-37. PubMed PMID: 12079332.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanism of action of the Escherichia coli phage shock protein PspA in repression of the AAA family transcription factor PspF. AU - Elderkin,Sarah, AU - Jones,Susan, AU - Schumacher,Jörg, AU - Studholme,David, AU - Buck,Martin, PY - 2002/6/25/pubmed PY - 2002/8/7/medline PY - 2002/6/25/entrez SP - 23 EP - 37 JF - Journal of molecular biology JO - J Mol Biol VL - 320 IS - 1 N2 - The PspA protein, a negative regulator of the Escherichia coli phage shock psp operon, is produced when virulence factors are exported through secretins in many Gram-negative pathogenic bacteria and its homologue in plants, VIPP1, plays a critical role in thylakoid biogenesis, essential for photosynthesis. Activation of transcription by the enhancer-dependent bacterial sigma(54) containing RNA polymerase occurs through ATP hydrolysis-driven protein conformational changes enabled by activator proteins that belong to the large AAA(+) mechanochemical protein family. We show that PspA directly and specifically acts upon and binds to the AAA(+) domain of the PspF transcription activator. Interactions involving PspF and nucleotide are changed by the action of PspA. These changes and the complexes that form between PspF and PspA can explain how PspA exerts its negative effects upon transcription activated by PspF, and are of significance when considering how activities of other AAA(+) proteins might be controlled. SN - 0022-2836 UR - https://www.unboundmedicine.com/medline/citation/12079332/Mechanism_of_action_of_the_Escherichia_coli_phage_shock_protein_PspA_in_repression_of_the_AAA_family_transcription_factor_PspF_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(02)00404-7 DB - PRIME DP - Unbound Medicine ER -