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The Srk1 protein kinase is a target for the Sty1 stress-activated MAPK in fission yeast.
J Biol Chem. 2002 Sep 06; 277(36):33411-21.JB

Abstract

The fission yeast stress-activated Sty1/Spc1 MAPK pathway responds to a similar range of stresses as do the mammalian p38 and SAPK/JNK MAPK pathways. In addition, sty1(-) cells are sterile and exhibit a G(2) cell cycle delay, indicating additional roles of Sty1 in meiosis and cell cycle progression. To identify novel proteins involved in stress responses, a microarray analysis of the Schizosaccharomyces pombe genome was performed to find genes that are up-regulated following exposure to stress in a Sty1-dependent manner. One such gene identified, srk1(+) (Sty1-regulated kinase 1), encodes a putative serine/threonine kinase homologous to mammalian calmodulin kinases. At the C terminus of Srk1 is a putative MAPK binding motif similar to that in the p38 substrates, MAPK-activated protein kinases 2 and 3. Indeed, we find that Srk1 is present in a complex with the Sty1 MAPK and is directly phosphorylated by Sty1. Furthermore, upon stress, Srk1 translocates from the cytoplasm to the nucleus in a process that is dependent on the Sty1 MAPK. Finally, we show that Srk1 has a role in regulating meiosis in fission yeast; following nitrogen limitation, srk1(-) cells enter meiosis significantly faster than wild-type cells and overexpression of srk1(+) inhibits the nitrogen starvation-induced arrest in G(1).

Authors+Show Affiliations

School of Biochemistry and Genetics, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12080074

Citation

Smith, Deborah A., et al. "The Srk1 Protein Kinase Is a Target for the Sty1 Stress-activated MAPK in Fission Yeast." The Journal of Biological Chemistry, vol. 277, no. 36, 2002, pp. 33411-21.
Smith DA, Toone WM, Chen D, et al. The Srk1 protein kinase is a target for the Sty1 stress-activated MAPK in fission yeast. J Biol Chem. 2002;277(36):33411-21.
Smith, D. A., Toone, W. M., Chen, D., Bahler, J., Jones, N., Morgan, B. A., & Quinn, J. (2002). The Srk1 protein kinase is a target for the Sty1 stress-activated MAPK in fission yeast. The Journal of Biological Chemistry, 277(36), 33411-21.
Smith DA, et al. The Srk1 Protein Kinase Is a Target for the Sty1 Stress-activated MAPK in Fission Yeast. J Biol Chem. 2002 Sep 6;277(36):33411-21. PubMed PMID: 12080074.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Srk1 protein kinase is a target for the Sty1 stress-activated MAPK in fission yeast. AU - Smith,Deborah A, AU - Toone,W Mark, AU - Chen,Dongrong, AU - Bahler,Jurg, AU - Jones,Nic, AU - Morgan,Brian A, AU - Quinn,Janet, Y1 - 2002/06/21/ PY - 2002/6/25/pubmed PY - 2002/10/31/medline PY - 2002/6/25/entrez SP - 33411 EP - 21 JF - The Journal of biological chemistry JO - J Biol Chem VL - 277 IS - 36 N2 - The fission yeast stress-activated Sty1/Spc1 MAPK pathway responds to a similar range of stresses as do the mammalian p38 and SAPK/JNK MAPK pathways. In addition, sty1(-) cells are sterile and exhibit a G(2) cell cycle delay, indicating additional roles of Sty1 in meiosis and cell cycle progression. To identify novel proteins involved in stress responses, a microarray analysis of the Schizosaccharomyces pombe genome was performed to find genes that are up-regulated following exposure to stress in a Sty1-dependent manner. One such gene identified, srk1(+) (Sty1-regulated kinase 1), encodes a putative serine/threonine kinase homologous to mammalian calmodulin kinases. At the C terminus of Srk1 is a putative MAPK binding motif similar to that in the p38 substrates, MAPK-activated protein kinases 2 and 3. Indeed, we find that Srk1 is present in a complex with the Sty1 MAPK and is directly phosphorylated by Sty1. Furthermore, upon stress, Srk1 translocates from the cytoplasm to the nucleus in a process that is dependent on the Sty1 MAPK. Finally, we show that Srk1 has a role in regulating meiosis in fission yeast; following nitrogen limitation, srk1(-) cells enter meiosis significantly faster than wild-type cells and overexpression of srk1(+) inhibits the nitrogen starvation-induced arrest in G(1). SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/12080074/The_Srk1_protein_kinase_is_a_target_for_the_Sty1_stress_activated_MAPK_in_fission_yeast_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(20)74501-1 DB - PRIME DP - Unbound Medicine ER -