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Gene transduction efficiency in cells of different species by HIV and EIAV vectors.
Gene Ther. 2002 Jul; 9(14):932-8.GT

Abstract

The ability of human immunodeficiency virus (HIV)- and equine infectious anaemia virus (EIAV)-based vectors to transduce cell lines from a range of species was compared. Both vectors carried the vesicular stomatitis virus G (VSV-G) envelope protein and encoded an enhanced green fluorescent protein (eGFP) gene driven by a human cytomegalovirus (CMV) early promoter. Immunostaining for viral core proteins and VSV-G was used to demonstrate that the HIV and EIAV vector preparations contained similar numbers of virus particles. Various cell lines were transduced with these vectors and the transduction efficiency was estimated by measuring eGFP expression. Efficient transduction by both vectors was observed in human, hamster, pig, horse, cat and dog cell lines, although EIAV vector was about 10-fold less efficient in human, hamster and pig cells normalised to the total number of viral particles. This could be partly explained by the lower RNA genome levels per particle for EIAV as measured by real-time RT-PCR. Rodent cells appeared to be transduced inefficiently with both vectors, but when the CMV promoter was substituted with the EF1alpha promoter in the HIV vectors, the expression level increased leading to an increase in the measurable level of transduction.

Authors+Show Affiliations

Department of Immunology and Molecular Pathology, Windeyer Institute of Medical Sciences, University College London, London, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12085241

Citation

Ikeda, Y, et al. "Gene Transduction Efficiency in Cells of Different Species By HIV and EIAV Vectors." Gene Therapy, vol. 9, no. 14, 2002, pp. 932-8.
Ikeda Y, Collins MK, Radcliffe PA, et al. Gene transduction efficiency in cells of different species by HIV and EIAV vectors. Gene Ther. 2002;9(14):932-8.
Ikeda, Y., Collins, M. K., Radcliffe, P. A., Mitrophanous, K. A., & Takeuchi, Y. (2002). Gene transduction efficiency in cells of different species by HIV and EIAV vectors. Gene Therapy, 9(14), 932-8.
Ikeda Y, et al. Gene Transduction Efficiency in Cells of Different Species By HIV and EIAV Vectors. Gene Ther. 2002;9(14):932-8. PubMed PMID: 12085241.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene transduction efficiency in cells of different species by HIV and EIAV vectors. AU - Ikeda,Y, AU - Collins,M K L, AU - Radcliffe,P A, AU - Mitrophanous,K A, AU - Takeuchi,Y, PY - 2002/01/10/received PY - 2002/02/18/accepted PY - 2002/6/27/pubmed PY - 2002/8/30/medline PY - 2002/6/27/entrez SP - 932 EP - 8 JF - Gene therapy JO - Gene Ther VL - 9 IS - 14 N2 - The ability of human immunodeficiency virus (HIV)- and equine infectious anaemia virus (EIAV)-based vectors to transduce cell lines from a range of species was compared. Both vectors carried the vesicular stomatitis virus G (VSV-G) envelope protein and encoded an enhanced green fluorescent protein (eGFP) gene driven by a human cytomegalovirus (CMV) early promoter. Immunostaining for viral core proteins and VSV-G was used to demonstrate that the HIV and EIAV vector preparations contained similar numbers of virus particles. Various cell lines were transduced with these vectors and the transduction efficiency was estimated by measuring eGFP expression. Efficient transduction by both vectors was observed in human, hamster, pig, horse, cat and dog cell lines, although EIAV vector was about 10-fold less efficient in human, hamster and pig cells normalised to the total number of viral particles. This could be partly explained by the lower RNA genome levels per particle for EIAV as measured by real-time RT-PCR. Rodent cells appeared to be transduced inefficiently with both vectors, but when the CMV promoter was substituted with the EF1alpha promoter in the HIV vectors, the expression level increased leading to an increase in the measurable level of transduction. SN - 0969-7128 UR - https://www.unboundmedicine.com/medline/citation/12085241/Gene_transduction_efficiency_in_cells_of_different_species_by_HIV_and_EIAV_vectors_ L2 - https://doi.org/10.1038/sj.gt.3301708 DB - PRIME DP - Unbound Medicine ER -