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Evaluation of a rapid real-time RT-PCR assay for detection of enterovirus RNA in cerebrospinal fluid specimens.
J Clin Virol. 2002 Jul; 25 Suppl 1:S39-43.JC

Abstract

BACKGROUND

We previously described the characteristics of a single-tube real-time enterovirus reverse transcriptase polymerase chain reaction (RT-PCR) assay based on a fluorogenic probe and primers directed to highly conserved sequences in the 5'-untranslated region (UTR) of the enterovirus genome.

OBJECTIVES

To evaluate the performance of the assay on a larger number of cerebrospinal fluid (CSF) specimens from patients suspected of having viral meningitis.

STUDY DESIGN

Real-time enterovirus RT-PCR and viral culture were performed on CSF specimens received from March 2000 to November 2001. Patient records were retrospectively reviewed for final clinical diagnosis.

RESULTS

From the 186 CSF specimens tested, culture was positive for enterovirus in 31 cases, whereas real-time RT-PCR detected enterovirus RNA in 45 CSF specimens. The sensitivity of real-time RT-PCR in relation to the clinical diagnosis of viral meningitis was 72.6%, whereas the sensitivity of viral culture reached only 57.4%. Enterovirus RNA was also found in a number of specimens with low leukocyte counts.

CONCLUSIONS

We confirm that the real-time enterovirus RT-PCR assay for CSF specimens is significantly more sensitive than viral culture.

Authors+Show Affiliations

Center for Molecular Diagnostics, OCMW Hospitals, AZ Middelheim, Lindendreef 1, B-2020 Antwerpen, Belgium.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

12091080

Citation

Verstrepen, W A., et al. "Evaluation of a Rapid Real-time RT-PCR Assay for Detection of Enterovirus RNA in Cerebrospinal Fluid Specimens." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 25 Suppl 1, 2002, pp. S39-43.
Verstrepen WA, Bruynseels P, Mertens AH. Evaluation of a rapid real-time RT-PCR assay for detection of enterovirus RNA in cerebrospinal fluid specimens. J Clin Virol. 2002;25 Suppl 1:S39-43.
Verstrepen, W. A., Bruynseels, P., & Mertens, A. H. (2002). Evaluation of a rapid real-time RT-PCR assay for detection of enterovirus RNA in cerebrospinal fluid specimens. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 25 Suppl 1, S39-43.
Verstrepen WA, Bruynseels P, Mertens AH. Evaluation of a Rapid Real-time RT-PCR Assay for Detection of Enterovirus RNA in Cerebrospinal Fluid Specimens. J Clin Virol. 2002;25 Suppl 1:S39-43. PubMed PMID: 12091080.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of a rapid real-time RT-PCR assay for detection of enterovirus RNA in cerebrospinal fluid specimens. AU - Verstrepen,W A, AU - Bruynseels,P, AU - Mertens,A H, PY - 2002/7/2/pubmed PY - 2003/2/14/medline PY - 2002/7/2/entrez SP - S39 EP - 43 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 25 Suppl 1 N2 - BACKGROUND: We previously described the characteristics of a single-tube real-time enterovirus reverse transcriptase polymerase chain reaction (RT-PCR) assay based on a fluorogenic probe and primers directed to highly conserved sequences in the 5'-untranslated region (UTR) of the enterovirus genome. OBJECTIVES: To evaluate the performance of the assay on a larger number of cerebrospinal fluid (CSF) specimens from patients suspected of having viral meningitis. STUDY DESIGN: Real-time enterovirus RT-PCR and viral culture were performed on CSF specimens received from March 2000 to November 2001. Patient records were retrospectively reviewed for final clinical diagnosis. RESULTS: From the 186 CSF specimens tested, culture was positive for enterovirus in 31 cases, whereas real-time RT-PCR detected enterovirus RNA in 45 CSF specimens. The sensitivity of real-time RT-PCR in relation to the clinical diagnosis of viral meningitis was 72.6%, whereas the sensitivity of viral culture reached only 57.4%. Enterovirus RNA was also found in a number of specimens with low leukocyte counts. CONCLUSIONS: We confirm that the real-time enterovirus RT-PCR assay for CSF specimens is significantly more sensitive than viral culture. SN - 1386-6532 UR - https://www.unboundmedicine.com/medline/citation/12091080/Evaluation_of_a_rapid_real_time_RT_PCR_assay_for_detection_of_enterovirus_RNA_in_cerebrospinal_fluid_specimens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S138665320200032X DB - PRIME DP - Unbound Medicine ER -