Tags

Type your tag names separated by a space and hit enter

Effects of arginine-enriched total parenteral nutrition on inflammatory-related mediator and T-cell population in septic rats.
Nutrition. 2002 Jul-Aug; 18(7-8):631-5.N

Abstract

OBJECTIVES

Previous reports have shown that oral arginine (Arg) has immune-enhancing properties in injury. However, the effects of parenterally infused Arg on sepsis are not well understood. We used a septic rat model to study Arg infusion in inflammatory-related cytokines and blood T lymphocyte population in vivo.

METHODS

Rats with internal jugular catheters were assigned to one of two groups. Both groups received isonitrogenous total parenteral nutrition (TPN) supplemented with 270 mg of nitrogen per kilogram per day as Arg or glycine (Gly). TPN provided 270 kcal/kg of body weight, and the kilocalorie:nitrogen ratio was 143:1. TPN was maintained for 5 d plus 2, 4, or 6 h or 6 d, according to the scheduled deaths of the rats. On day 5, sepsis was induced by cecal ligation and puncture (CLP). After CLP for 2, 4, 6, and 24 h, rats were killed.

RESULTS

The results showed that interleukin-1beta and tumor necrosis factor-alpha concentrations in peritoneal lavage fluid at 6 h and interleukin-6 levels at 24 h after CLP in the Gly group were significantly higher than those in the Arg group. The T-lymphocyte population in blood showed that CD8(+) suppressor T-cell number was significantly higher in the Gly group than in the Arg group at 6 h after CLP. The blood CD4(+):CD8(+) ratio was significantly higher in the Arg group than in the Gly group at 24 h after CLP. A negative nitrogen balance was observed in the Arg and Gly groups after CLP; there was no significant difference in nitrogen balance between the septic groups. No difference in survival rate at 24 h after CLP was observed between the groups.

CONCLUSIONS

The results showed that, compared with the Gly group, TPN preinfused with Arg reduces the production of inflammatory mediators at the site of injury and that cellular immunity is enhanced at 24 h after CLP. Parenterally administered Arg had no beneficial effect in preventing nitrogen loss and improving survival in septic rats. Whether Gly has specific effects that reduce the effects of Arg require further investigation.

Authors+Show Affiliations

Institute of Nutrition and Health Science, Taipei Medical University, Taipei, Taiwan, Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12093444

Citation

Yeh, Chiu-Li, et al. "Effects of Arginine-enriched Total Parenteral Nutrition On Inflammatory-related Mediator and T-cell Population in Septic Rats." Nutrition (Burbank, Los Angeles County, Calif.), vol. 18, no. 7-8, 2002, pp. 631-5.
Yeh CL, Yeh SL, Lin MT, et al. Effects of arginine-enriched total parenteral nutrition on inflammatory-related mediator and T-cell population in septic rats. Nutrition. 2002;18(7-8):631-5.
Yeh, C. L., Yeh, S. L., Lin, M. T., & Chen, W. J. (2002). Effects of arginine-enriched total parenteral nutrition on inflammatory-related mediator and T-cell population in septic rats. Nutrition (Burbank, Los Angeles County, Calif.), 18(7-8), 631-5.
Yeh CL, et al. Effects of Arginine-enriched Total Parenteral Nutrition On Inflammatory-related Mediator and T-cell Population in Septic Rats. Nutrition. 2002 Jul-Aug;18(7-8):631-5. PubMed PMID: 12093444.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of arginine-enriched total parenteral nutrition on inflammatory-related mediator and T-cell population in septic rats. AU - Yeh,Chiu-Li, AU - Yeh,Sung-Ling, AU - Lin,Ming-Tsan, AU - Chen,Wei-Jao, PY - 2002/7/3/pubmed PY - 2002/8/10/medline PY - 2002/7/3/entrez SP - 631 EP - 5 JF - Nutrition (Burbank, Los Angeles County, Calif.) JO - Nutrition VL - 18 IS - 7-8 N2 - OBJECTIVES: Previous reports have shown that oral arginine (Arg) has immune-enhancing properties in injury. However, the effects of parenterally infused Arg on sepsis are not well understood. We used a septic rat model to study Arg infusion in inflammatory-related cytokines and blood T lymphocyte population in vivo. METHODS: Rats with internal jugular catheters were assigned to one of two groups. Both groups received isonitrogenous total parenteral nutrition (TPN) supplemented with 270 mg of nitrogen per kilogram per day as Arg or glycine (Gly). TPN provided 270 kcal/kg of body weight, and the kilocalorie:nitrogen ratio was 143:1. TPN was maintained for 5 d plus 2, 4, or 6 h or 6 d, according to the scheduled deaths of the rats. On day 5, sepsis was induced by cecal ligation and puncture (CLP). After CLP for 2, 4, 6, and 24 h, rats were killed. RESULTS: The results showed that interleukin-1beta and tumor necrosis factor-alpha concentrations in peritoneal lavage fluid at 6 h and interleukin-6 levels at 24 h after CLP in the Gly group were significantly higher than those in the Arg group. The T-lymphocyte population in blood showed that CD8(+) suppressor T-cell number was significantly higher in the Gly group than in the Arg group at 6 h after CLP. The blood CD4(+):CD8(+) ratio was significantly higher in the Arg group than in the Gly group at 24 h after CLP. A negative nitrogen balance was observed in the Arg and Gly groups after CLP; there was no significant difference in nitrogen balance between the septic groups. No difference in survival rate at 24 h after CLP was observed between the groups. CONCLUSIONS: The results showed that, compared with the Gly group, TPN preinfused with Arg reduces the production of inflammatory mediators at the site of injury and that cellular immunity is enhanced at 24 h after CLP. Parenterally administered Arg had no beneficial effect in preventing nitrogen loss and improving survival in septic rats. Whether Gly has specific effects that reduce the effects of Arg require further investigation. SN - 0899-9007 UR - https://www.unboundmedicine.com/medline/citation/12093444/Effects_of_arginine_enriched_total_parenteral_nutrition_on_inflammatory_related_mediator_and_T_cell_population_in_septic_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0899900702008092 DB - PRIME DP - Unbound Medicine ER -