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Eosinophils express functional IL-13 in eosinophilic inflammatory diseases.
J Immunol. 2002 Jul 15; 169(2):1021-7.JI

Abstract

IL-13 is an immunoregulatory and effector cytokine in allergic diseases such as bronchial asthma. A variety of immune and non-immune cells are known as IL-13 producers. In this study we investigated whether and under what conditions human eosinophils generate IL-13. Freshly isolated highly purified peripheral blood eosinophils from patients with several eosinophilic inflammatory diseases and from normal control individuals were investigated. We observed that blood eosinophils from patients suffering from bronchial asthma, atopic dermatitis, parasitic infections, hypereosinophilic syndrome, and idiopathic eosinophilic esophagitis expressed IL-13, as assessed by ELISA, ELISPOT assay, flow cytometry, and immunocytochemistry. By using nasal polyp tissues and immunohistochemistry, we demonstrated IL-13 expression in eosinophils under in vivo conditions. In contrast, blood eosinophils from control individuals as well as blood neutrophils from both eosinophilic and control patients did not produce detectable IL-13 levels. However, when blood eosinophils from control individuals were stimulated with GM-CSF or IL-5 in vitro, they generated IL-13 mRNA and protein, suggesting that IL-13 expression by eosinophils under inflammatory conditions is a cytokine-driven process. Stimulation of blood eosinophils containing IL-13 by eotaxin resulted in a rapid release of this cytokine. Eosinophil-derived IL-13 was functional, as it increased the surface expression of the low affinity IgE receptor (CD23) on purified B cells. In conclusion, human eosinophils are able to produce and release functional IL-13 in eosinophilic inflammatory responses.

Authors+Show Affiliations

Swiss Institute for Allergy and Asthma Research, Davos-Platz, Switzerland. peter.schmid@hin.chNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12097410

Citation

Schmid-Grendelmeier, Peter, et al. "Eosinophils Express Functional IL-13 in Eosinophilic Inflammatory Diseases." Journal of Immunology (Baltimore, Md. : 1950), vol. 169, no. 2, 2002, pp. 1021-7.
Schmid-Grendelmeier P, Altznauer F, Fischer B, et al. Eosinophils express functional IL-13 in eosinophilic inflammatory diseases. J Immunol. 2002;169(2):1021-7.
Schmid-Grendelmeier, P., Altznauer, F., Fischer, B., Bizer, C., Straumann, A., Menz, G., Blaser, K., Wüthrich, B., & Simon, H. U. (2002). Eosinophils express functional IL-13 in eosinophilic inflammatory diseases. Journal of Immunology (Baltimore, Md. : 1950), 169(2), 1021-7.
Schmid-Grendelmeier P, et al. Eosinophils Express Functional IL-13 in Eosinophilic Inflammatory Diseases. J Immunol. 2002 Jul 15;169(2):1021-7. PubMed PMID: 12097410.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Eosinophils express functional IL-13 in eosinophilic inflammatory diseases. AU - Schmid-Grendelmeier,Peter, AU - Altznauer,Frank, AU - Fischer,Barbra, AU - Bizer,Christian, AU - Straumann,Alex, AU - Menz,Günter, AU - Blaser,Kurt, AU - Wüthrich,Brunello, AU - Simon,Hans-Uwe, PY - 2002/7/5/pubmed PY - 2002/8/29/medline PY - 2002/7/5/entrez SP - 1021 EP - 7 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 169 IS - 2 N2 - IL-13 is an immunoregulatory and effector cytokine in allergic diseases such as bronchial asthma. A variety of immune and non-immune cells are known as IL-13 producers. In this study we investigated whether and under what conditions human eosinophils generate IL-13. Freshly isolated highly purified peripheral blood eosinophils from patients with several eosinophilic inflammatory diseases and from normal control individuals were investigated. We observed that blood eosinophils from patients suffering from bronchial asthma, atopic dermatitis, parasitic infections, hypereosinophilic syndrome, and idiopathic eosinophilic esophagitis expressed IL-13, as assessed by ELISA, ELISPOT assay, flow cytometry, and immunocytochemistry. By using nasal polyp tissues and immunohistochemistry, we demonstrated IL-13 expression in eosinophils under in vivo conditions. In contrast, blood eosinophils from control individuals as well as blood neutrophils from both eosinophilic and control patients did not produce detectable IL-13 levels. However, when blood eosinophils from control individuals were stimulated with GM-CSF or IL-5 in vitro, they generated IL-13 mRNA and protein, suggesting that IL-13 expression by eosinophils under inflammatory conditions is a cytokine-driven process. Stimulation of blood eosinophils containing IL-13 by eotaxin resulted in a rapid release of this cytokine. Eosinophil-derived IL-13 was functional, as it increased the surface expression of the low affinity IgE receptor (CD23) on purified B cells. In conclusion, human eosinophils are able to produce and release functional IL-13 in eosinophilic inflammatory responses. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/12097410/Eosinophils_express_functional_IL_13_in_eosinophilic_inflammatory_diseases_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=12097410 DB - PRIME DP - Unbound Medicine ER -