Tags

Type your tag names separated by a space and hit enter

Selenium influences the turnover of selenocysteine tRNA([Ser]Sec) in Chinese hamster ovary cells.
J Nutr. 2002 Jul; 132(7):1830-5.JN

Abstract

Selenocysteine transfer RNA (tRNA([Ser]Sec)) is a central molecule in the production of selenium-containing proteins, and may play a role in the regulation of their biosynthesis. Selenium concentration influences both the levels of tRNA([Ser]Sec) and the relative abundance of two isoforms. To study the mechanism by which selenium affects tRNA([Ser]Sec) levels, Chinese hamster ovary (CHO) cells were treated with the transcription inhibitor, actinomycin D, and tRNA([Ser]Sec) levels were determined by Northern blotting, primer extension and reverse-phase column chromatography. Turnover of tRNA([Ser]Sec) in CHO cells was faster than the total tRNA population. Supplementation of the culture media with selenium reduced turnover of tRNA([Ser]Sec), but did not influence turnover of a randomly selected serine tRNA. Inhibition of transcription with actinomycin D resulted in a relative increase in the abundance of the isoform containing methylcarboxymethyl-5'-uridine-2'-O-methylribose in the wobble position of the anticodon. Primer extension studies, which permitted the independent evaluation of the tRNA([Ser]Sec) arising from the introduced mouse gene and that derived from the host CHO gene, indicated an accelerated decline in tRNA([Ser]Sec) derived from both the transfected and the native gene. These results provide additional insight into the levels of regulation that control the translation of selenium containing proteins in mammalian cells.

Authors+Show Affiliations

Department of Human Nutrition, University of Illinois at Chicago, 60612, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12097655

Citation

Jameson, Ruth R., et al. "Selenium Influences the Turnover of Selenocysteine tRNA([Ser]Sec) in Chinese Hamster Ovary Cells." The Journal of Nutrition, vol. 132, no. 7, 2002, pp. 1830-5.
Jameson RR, Carlson BA, Butz M, et al. Selenium influences the turnover of selenocysteine tRNA([Ser]Sec) in Chinese hamster ovary cells. J Nutr. 2002;132(7):1830-5.
Jameson, R. R., Carlson, B. A., Butz, M., Esser, K., Hatfield, D. L., & Diamond, A. M. (2002). Selenium influences the turnover of selenocysteine tRNA([Ser]Sec) in Chinese hamster ovary cells. The Journal of Nutrition, 132(7), 1830-5.
Jameson RR, et al. Selenium Influences the Turnover of Selenocysteine tRNA([Ser]Sec) in Chinese Hamster Ovary Cells. J Nutr. 2002;132(7):1830-5. PubMed PMID: 12097655.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selenium influences the turnover of selenocysteine tRNA([Ser]Sec) in Chinese hamster ovary cells. AU - Jameson,Ruth R, AU - Carlson,Bradley A, AU - Butz,Michael, AU - Esser,Karyn, AU - Hatfield,Dolph L, AU - Diamond,Alan M, PY - 2002/7/5/pubmed PY - 2002/7/27/medline PY - 2002/7/5/entrez SP - 1830 EP - 5 JF - The Journal of nutrition JO - J. Nutr. VL - 132 IS - 7 N2 - Selenocysteine transfer RNA (tRNA([Ser]Sec)) is a central molecule in the production of selenium-containing proteins, and may play a role in the regulation of their biosynthesis. Selenium concentration influences both the levels of tRNA([Ser]Sec) and the relative abundance of two isoforms. To study the mechanism by which selenium affects tRNA([Ser]Sec) levels, Chinese hamster ovary (CHO) cells were treated with the transcription inhibitor, actinomycin D, and tRNA([Ser]Sec) levels were determined by Northern blotting, primer extension and reverse-phase column chromatography. Turnover of tRNA([Ser]Sec) in CHO cells was faster than the total tRNA population. Supplementation of the culture media with selenium reduced turnover of tRNA([Ser]Sec), but did not influence turnover of a randomly selected serine tRNA. Inhibition of transcription with actinomycin D resulted in a relative increase in the abundance of the isoform containing methylcarboxymethyl-5'-uridine-2'-O-methylribose in the wobble position of the anticodon. Primer extension studies, which permitted the independent evaluation of the tRNA([Ser]Sec) arising from the introduced mouse gene and that derived from the host CHO gene, indicated an accelerated decline in tRNA([Ser]Sec) derived from both the transfected and the native gene. These results provide additional insight into the levels of regulation that control the translation of selenium containing proteins in mammalian cells. SN - 0022-3166 UR - https://www.unboundmedicine.com/medline/citation/12097655/Selenium_influences_the_turnover_of_selenocysteine_tRNA_[Ser]Sec__in_Chinese_hamster_ovary_cells_ L2 - https://academic.oup.com/jn/article-lookup/doi/10.1093/jn/132.7.1830 DB - PRIME DP - Unbound Medicine ER -