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Synthesis and oxidative refolding of hainantoxin-IV.

Abstract

Hainantoxin-IV, a neutoxic peptide from the spider Selenocosimia hainana, was synthesized by solid-phase method with fluorenylmethyoxycarbonyl amino acids (Fmoc-AA). Reverse-phase HPLC was used to monitor the oxidative folding of synthetic Hainantoxin-IV under different reaction conditions in order to find optimal conditions for renaturation of synthetic Hainantoxin-IV. The best renaturation yield was received in 5 mmol/L GSH and 0.5 mmol/L GSSG at pH 8.0 in 0.1 mol/L Tris-HCl and 0.1 mol/L NaCl buffer. The renaturated Hainantoxin-IV was monitored with MALDI-TOF MS reverse-phase HPLC and isolated mouse phrenic nerve-diaphragm preparation.

Authors+Show Affiliations

College of Life Science of Hunan Normal University, Changsha 410081, China. liangsp@public.cs.hn.cnNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12098779

Citation

Liu, Zhong-Hua, et al. "Synthesis and Oxidative Refolding of Hainantoxin-IV." Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica, vol. 34, no. 4, 2002, pp. 516-9.
Liu ZH, Chen P, Liang SP. Synthesis and oxidative refolding of hainantoxin-IV. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2002;34(4):516-9.
Liu, Z. H., Chen, P., & Liang, S. P. (2002). Synthesis and oxidative refolding of hainantoxin-IV. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica, 34(4), 516-9.
Liu ZH, Chen P, Liang SP. Synthesis and Oxidative Refolding of Hainantoxin-IV. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2002;34(4):516-9. PubMed PMID: 12098779.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis and oxidative refolding of hainantoxin-IV. AU - Liu,Zhong-Hua, AU - Chen,Ping, AU - Liang,Song-Ping, PY - 2002/7/6/pubmed PY - 2002/8/28/medline PY - 2002/7/6/entrez SP - 516 EP - 9 JF - Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica JO - Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) VL - 34 IS - 4 N2 - Hainantoxin-IV, a neutoxic peptide from the spider Selenocosimia hainana, was synthesized by solid-phase method with fluorenylmethyoxycarbonyl amino acids (Fmoc-AA). Reverse-phase HPLC was used to monitor the oxidative folding of synthetic Hainantoxin-IV under different reaction conditions in order to find optimal conditions for renaturation of synthetic Hainantoxin-IV. The best renaturation yield was received in 5 mmol/L GSH and 0.5 mmol/L GSSG at pH 8.0 in 0.1 mol/L Tris-HCl and 0.1 mol/L NaCl buffer. The renaturated Hainantoxin-IV was monitored with MALDI-TOF MS reverse-phase HPLC and isolated mouse phrenic nerve-diaphragm preparation. SN - 0582-9879 UR - https://www.unboundmedicine.com/medline/citation/12098779/Synthesis_and_oxidative_refolding_of_hainantoxin_IV_ DB - PRIME DP - Unbound Medicine ER -