Tags

Type your tag names separated by a space and hit enter

A comparison of 13 guinea pig and human anti-tissue transglutaminase antibody ELISA kits.
J Clin Pathol 2002; 55(7):488-94JC

Abstract

AIMS

Tissue transglutaminase (tTG) is a major autoantigen recognised by IgA anti-endomysial antibodies (IgA EMA). Enzyme linked immunosorbent assays (ELISA) for IgA anti-tissue transglutaminase antibodies (IgA tTG) have therefore been developed as an alternative serological screening test to IgA EMA for coeliac disease (CD). The use of human tTG (h-tTG), as opposed to guinea pig liver tTG (gpl-tTG), in these assays has been reported to produce superior results. This study compared 13 commercial IgA tTG ELISA kits to ascertain their performance characteristics in the diagnosis of CD in patients with biopsy confirmed disease compared with controls. All patients and controls were adults aged 21 years or older.

METHODS

Sera from the following groups of patients were tested in each kit: (1) 49 patients with CD confirmed on small bowel biopsies (all IgA EMA positive); (2) 34 patients with small bowel biopsies that were not consistent with CD; and (3) 30 patients with biopsy confirmed inflammatory bowel disease. All controls were negative for IgA EMA and were not IgA deficient. Sensitivities and specificities were determined using both the manufacturers' recommended cut off points and receiver operating characteristic (ROC) analysis derived decision thresholds. The area under the curve (AUC) for each ROC plot was also calculated and compared between kits.

RESULTS

In general, the h-tTG based IgA tTG ELISA kits demonstrated superior performance (especially specificity) compared with the gpl-tTG based kits, although 100% sensitivity and specificity (comparable to the IgA EMA assay) was obtained in only one recombinant h-tTG based kit.

CONCLUSIONS

The use of h-tTG in IgA tTG ELISA kits is generally, but not universally, associated with superior performance. Factors other than antigen source are important in determining kit performance.

Authors+Show Affiliations

Division of Immunology, Queensland Health Pathology Services, Princess Alexandra and Royal Brisbane Hospitals, Australia. richard_wong@health.qld.gov.auNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Multicenter Study

Language

eng

PubMed ID

12101191

Citation

Wong, R C W., et al. "A Comparison of 13 Guinea Pig and Human Anti-tissue Transglutaminase Antibody ELISA Kits." Journal of Clinical Pathology, vol. 55, no. 7, 2002, pp. 488-94.
Wong RC, Wilson RJ, Steele RH, et al. A comparison of 13 guinea pig and human anti-tissue transglutaminase antibody ELISA kits. J Clin Pathol. 2002;55(7):488-94.
Wong, R. C., Wilson, R. J., Steele, R. H., Radford-Smith, G., & Adelstein, S. (2002). A comparison of 13 guinea pig and human anti-tissue transglutaminase antibody ELISA kits. Journal of Clinical Pathology, 55(7), pp. 488-94.
Wong RC, et al. A Comparison of 13 Guinea Pig and Human Anti-tissue Transglutaminase Antibody ELISA Kits. J Clin Pathol. 2002;55(7):488-94. PubMed PMID: 12101191.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A comparison of 13 guinea pig and human anti-tissue transglutaminase antibody ELISA kits. AU - Wong,R C W, AU - Wilson,R J, AU - Steele,R H, AU - Radford-Smith,G, AU - Adelstein,S, PY - 2002/7/9/pubmed PY - 2002/8/30/medline PY - 2002/7/9/entrez SP - 488 EP - 94 JF - Journal of clinical pathology JO - J. Clin. Pathol. VL - 55 IS - 7 N2 - AIMS: Tissue transglutaminase (tTG) is a major autoantigen recognised by IgA anti-endomysial antibodies (IgA EMA). Enzyme linked immunosorbent assays (ELISA) for IgA anti-tissue transglutaminase antibodies (IgA tTG) have therefore been developed as an alternative serological screening test to IgA EMA for coeliac disease (CD). The use of human tTG (h-tTG), as opposed to guinea pig liver tTG (gpl-tTG), in these assays has been reported to produce superior results. This study compared 13 commercial IgA tTG ELISA kits to ascertain their performance characteristics in the diagnosis of CD in patients with biopsy confirmed disease compared with controls. All patients and controls were adults aged 21 years or older. METHODS: Sera from the following groups of patients were tested in each kit: (1) 49 patients with CD confirmed on small bowel biopsies (all IgA EMA positive); (2) 34 patients with small bowel biopsies that were not consistent with CD; and (3) 30 patients with biopsy confirmed inflammatory bowel disease. All controls were negative for IgA EMA and were not IgA deficient. Sensitivities and specificities were determined using both the manufacturers' recommended cut off points and receiver operating characteristic (ROC) analysis derived decision thresholds. The area under the curve (AUC) for each ROC plot was also calculated and compared between kits. RESULTS: In general, the h-tTG based IgA tTG ELISA kits demonstrated superior performance (especially specificity) compared with the gpl-tTG based kits, although 100% sensitivity and specificity (comparable to the IgA EMA assay) was obtained in only one recombinant h-tTG based kit. CONCLUSIONS: The use of h-tTG in IgA tTG ELISA kits is generally, but not universally, associated with superior performance. Factors other than antigen source are important in determining kit performance. SN - 0021-9746 UR - https://www.unboundmedicine.com/medline/citation/12101191/A_comparison_of_13_guinea_pig_and_human_anti_tissue_transglutaminase_antibody_ELISA_kits_ L2 - http://jcp.bmj.com/cgi/pmidlookup?view=long&pmid=12101191 DB - PRIME DP - Unbound Medicine ER -