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Indirect allorecognition of mismatched donor HLA class II peptides in lung transplant recipients with bronchiolitis obliterans syndrome.
Am J Transplant. 2001 Sep; 1(3):228-35.AJ

Abstract

A correlation between indirect allorecognition of mismatched donor HLA class I peptides and development of bronchiolitis obliterans syndrome (BOS) after lung transplantation has been previously observed. The aim of this study was to determine whether there was a correlation between indirect allorecognition of mismatched donor HLA class II peptides and development of BOS after lung transplantation. Peripheral blood mononuclear cells from nine BOS+ and nine BOS-lung transplant recipients were cultured with synthetic peptides corresponding to the beta-chain hypervariable region of a mismatched donor HLA-DR molecule. Then, proliferative alloreactivity as well as frequency of alloreactive T cells were determined. In addition, the immunodominant epitopes from the donor HLA-DR molecules were identified in selected patients. T cells from BOS+ patients showed a dose-dependent proliferative alloreactivity against donor HLA-DR peptides that was significantly higher than that observed in BOS- patients (p=0.001). Similarly, the frequency of HLA-DR alloreactive T cells was significantly higher in BOS+ patients than in BOS- patients (p=0.001). This T-cell alloreactivity was directed against a single immunodominant HLA-DR peptide. These results suggest that indirect alloreactivity to donor HLA class II molecules may play a role in the pathogenesis of BOS after lung transplantation.

Authors+Show Affiliations

Department of Surgery, Washington University School of Medicine, St. Louis, Missouri 63110, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12102256

Citation

Reznik, S I., et al. "Indirect Allorecognition of Mismatched Donor HLA Class II Peptides in Lung Transplant Recipients With Bronchiolitis Obliterans Syndrome." American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons, vol. 1, no. 3, 2001, pp. 228-35.
Reznik SI, Jaramillo A, SivaSai KS, et al. Indirect allorecognition of mismatched donor HLA class II peptides in lung transplant recipients with bronchiolitis obliterans syndrome. Am J Transplant. 2001;1(3):228-35.
Reznik, S. I., Jaramillo, A., SivaSai, K. S., Womer, K. L., Sayegh, M. H., Trulock, E. P., Patterson, G. A., & Mohanakumar, T. (2001). Indirect allorecognition of mismatched donor HLA class II peptides in lung transplant recipients with bronchiolitis obliterans syndrome. American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 1(3), 228-35.
Reznik SI, et al. Indirect Allorecognition of Mismatched Donor HLA Class II Peptides in Lung Transplant Recipients With Bronchiolitis Obliterans Syndrome. Am J Transplant. 2001;1(3):228-35. PubMed PMID: 12102256.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Indirect allorecognition of mismatched donor HLA class II peptides in lung transplant recipients with bronchiolitis obliterans syndrome. AU - Reznik,S I, AU - Jaramillo,A, AU - SivaSai,K S, AU - Womer,K L, AU - Sayegh,M H, AU - Trulock,E P, AU - Patterson,G A, AU - Mohanakumar,T, PY - 2002/7/10/pubmed PY - 2002/8/1/medline PY - 2002/7/10/entrez SP - 228 EP - 35 JF - American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons JO - Am. J. Transplant. VL - 1 IS - 3 N2 - A correlation between indirect allorecognition of mismatched donor HLA class I peptides and development of bronchiolitis obliterans syndrome (BOS) after lung transplantation has been previously observed. The aim of this study was to determine whether there was a correlation between indirect allorecognition of mismatched donor HLA class II peptides and development of BOS after lung transplantation. Peripheral blood mononuclear cells from nine BOS+ and nine BOS-lung transplant recipients were cultured with synthetic peptides corresponding to the beta-chain hypervariable region of a mismatched donor HLA-DR molecule. Then, proliferative alloreactivity as well as frequency of alloreactive T cells were determined. In addition, the immunodominant epitopes from the donor HLA-DR molecules were identified in selected patients. T cells from BOS+ patients showed a dose-dependent proliferative alloreactivity against donor HLA-DR peptides that was significantly higher than that observed in BOS- patients (p=0.001). Similarly, the frequency of HLA-DR alloreactive T cells was significantly higher in BOS+ patients than in BOS- patients (p=0.001). This T-cell alloreactivity was directed against a single immunodominant HLA-DR peptide. These results suggest that indirect alloreactivity to donor HLA class II molecules may play a role in the pathogenesis of BOS after lung transplantation. SN - 1600-6135 UR - https://www.unboundmedicine.com/medline/citation/12102256/Indirect_allorecognition_of_mismatched_donor_HLA_class_II_peptides_in_lung_transplant_recipients_with_bronchiolitis_obliterans_syndrome_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=1600-6135&date=2001&volume=1&issue=3&spage=228 DB - PRIME DP - Unbound Medicine ER -