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Development of a biocatalytic process for the production of c6-aldehydes from vegetable oils by soybean lipoxygenase and recombinant hydroperoxide lyase.
J Agric Food Chem. 2002 Jul 17; 50(15):4270-4.JA

Abstract

Volatile C6- and C9-aldehydes and alcohols are widely used as food flavors to reconstitute the "fresh green" odor of fruits and vegetables lost during processing. To meet the high demand for natural flavors, an efficient, cheap, and versatile biocatalytic process was developed to produce C6-aldehydes on a large scale. Vegetable oils were converted by soybean lipoxygenase and recombinant hydroperoxide lyase into hexanal and (2E)- or (3Z)-hexenal. In contrast to plant extracts, generally used as enzyme sources, high molar conversions were obtained with recombinant hydroperoxide lyase (50% for hexanal and 26% for hexenal formation), and no side products were formed. Furthermore, recombinant hydroperoxide lyase lacks isomerase activity, allowing production of (3Z)-hexenal, which could not be obtained in previously described processes. Recombinant hydroperoxide lyase is stable and can be stored at 4 degrees C for 1 month without significant loss of activity.

Authors+Show Affiliations

Bijvoet Center for Biomolecular Research, Department of Bio-organic Chemistry, Padualaan 8, 3584 CH Utrecht, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12105957

Citation

Noordermeer, Minke A., et al. "Development of a Biocatalytic Process for the Production of C6-aldehydes From Vegetable Oils By Soybean Lipoxygenase and Recombinant Hydroperoxide Lyase." Journal of Agricultural and Food Chemistry, vol. 50, no. 15, 2002, pp. 4270-4.
Noordermeer MA, Van Der Goot W, Van Kooij AJ, et al. Development of a biocatalytic process for the production of c6-aldehydes from vegetable oils by soybean lipoxygenase and recombinant hydroperoxide lyase. J Agric Food Chem. 2002;50(15):4270-4.
Noordermeer, M. A., Van Der Goot, W., Van Kooij, A. J., Veldsink, J. W., Veldink, G. A., & Vliegenthart, J. F. (2002). Development of a biocatalytic process for the production of c6-aldehydes from vegetable oils by soybean lipoxygenase and recombinant hydroperoxide lyase. Journal of Agricultural and Food Chemistry, 50(15), 4270-4.
Noordermeer MA, et al. Development of a Biocatalytic Process for the Production of C6-aldehydes From Vegetable Oils By Soybean Lipoxygenase and Recombinant Hydroperoxide Lyase. J Agric Food Chem. 2002 Jul 17;50(15):4270-4. PubMed PMID: 12105957.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a biocatalytic process for the production of c6-aldehydes from vegetable oils by soybean lipoxygenase and recombinant hydroperoxide lyase. AU - Noordermeer,Minke A, AU - Van Der Goot,Wouter, AU - Van Kooij,Aukje J, AU - Veldsink,Jan Willem, AU - Veldink,Gerrit A, AU - Vliegenthart,Johannes F G, PY - 2002/7/11/pubmed PY - 2002/8/21/medline PY - 2002/7/11/entrez SP - 4270 EP - 4 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 50 IS - 15 N2 - Volatile C6- and C9-aldehydes and alcohols are widely used as food flavors to reconstitute the "fresh green" odor of fruits and vegetables lost during processing. To meet the high demand for natural flavors, an efficient, cheap, and versatile biocatalytic process was developed to produce C6-aldehydes on a large scale. Vegetable oils were converted by soybean lipoxygenase and recombinant hydroperoxide lyase into hexanal and (2E)- or (3Z)-hexenal. In contrast to plant extracts, generally used as enzyme sources, high molar conversions were obtained with recombinant hydroperoxide lyase (50% for hexanal and 26% for hexenal formation), and no side products were formed. Furthermore, recombinant hydroperoxide lyase lacks isomerase activity, allowing production of (3Z)-hexenal, which could not be obtained in previously described processes. Recombinant hydroperoxide lyase is stable and can be stored at 4 degrees C for 1 month without significant loss of activity. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/12105957/Development_of_a_biocatalytic_process_for_the_production_of_c6_aldehydes_from_vegetable_oils_by_soybean_lipoxygenase_and_recombinant_hydroperoxide_lyase_ DB - PRIME DP - Unbound Medicine ER -