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Establishment and characterisation of six human biliary tract cancer cell lines.
Br J Cancer 2002; 87(2):187-93BJ

Abstract

Human cell lines established from biliary tract cancers are rare, and only five have been reported previously. We report the characterisation of six new six biliary tract cancer cell lines (designated SNU-245, SNU-308, SNU-478, SNU-869, SNU-1079 and SNU-1196) established from primary tumour samples of Korean patients. The cell lines were isolated from two extrahepatic bile duct cancers (one adenocarcinoma of common bile duct, one hilar bile duct cancer), two adenocarcinomas of ampulla of Vater, one intrahepatic bile duct cancer (cholangiocarcinoma), and one adenocarcinoma of the gall bladder. The cell phenotypes, including the histopathology of the primary tumours and in vitro growth characteristics, were determined. We also performed molecular characterisation, including DNA fingerprinting analysis and abnormalities of K-ras, p15, p16, p53, hMLH1, hMSH2, DPC4, beta-catenin, E-cadherin, hOGG1, STK11, and TGF-betaRII genes by PCR-SSCP and sequencing analysis. In addition, we compared the genetic alterations in tumour cell lines and their corresponding tumour tissues. All lines grew as adherent cells. Population doubling times varied from 48-72 h. The culture success rate was 20% (six out of 30 attempts). All cell lines showed (i) relatively high viability; (ii) absence of mycoplasma or bacteria contamination; and (iii) genetic heterogeneity by DNA fingerprinting analysis. Among the lines, three lines had p53 mutations; and homozygous deletions in both p16 and p15 genes were found three and three lines, respectively; one line had a heterozygous missense mutation in hMLH1; E-cadherin gene was hypermethylated in two lines. Since the establishment of biliary tract cancer cell lines has been rarely reported in the literature, these newly established and well characterised biliary tract cancer cell lines would be very useful for studying the biology of biliary tract cancers, particularly those related to hypermethylation of E-cadherin gene in biliary tract cancer.

Authors+Show Affiliations

Laboratory of Cell Biology, Korean Cell Line Bank, Cancer Research Center and Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-744, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12107841

Citation

Ku, J-L, et al. "Establishment and Characterisation of Six Human Biliary Tract Cancer Cell Lines." British Journal of Cancer, vol. 87, no. 2, 2002, pp. 187-93.
Ku JL, Yoon KA, Kim IJ, et al. Establishment and characterisation of six human biliary tract cancer cell lines. Br J Cancer. 2002;87(2):187-93.
Ku, J. L., Yoon, K. A., Kim, I. J., Kim, W. H., Jang, J. Y., Suh, K. S., ... Park, J. G. (2002). Establishment and characterisation of six human biliary tract cancer cell lines. British Journal of Cancer, 87(2), pp. 187-93.
Ku JL, et al. Establishment and Characterisation of Six Human Biliary Tract Cancer Cell Lines. Br J Cancer. 2002 Jul 15;87(2):187-93. PubMed PMID: 12107841.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Establishment and characterisation of six human biliary tract cancer cell lines. AU - Ku,J-L, AU - Yoon,K-A, AU - Kim,I-J, AU - Kim,W-H, AU - Jang,J-Y, AU - Suh,K-S, AU - Kim,S-W, AU - Park,Y-H, AU - Hwang,J-H, AU - Yoon,Y-B, AU - Park,J-G, PY - 2001/12/24/received PY - 2002/04/15/revised PY - 2002/05/08/accepted PY - 2002/7/11/pubmed PY - 2002/8/31/medline PY - 2002/7/11/entrez SP - 187 EP - 93 JF - British journal of cancer JO - Br. J. Cancer VL - 87 IS - 2 N2 - Human cell lines established from biliary tract cancers are rare, and only five have been reported previously. We report the characterisation of six new six biliary tract cancer cell lines (designated SNU-245, SNU-308, SNU-478, SNU-869, SNU-1079 and SNU-1196) established from primary tumour samples of Korean patients. The cell lines were isolated from two extrahepatic bile duct cancers (one adenocarcinoma of common bile duct, one hilar bile duct cancer), two adenocarcinomas of ampulla of Vater, one intrahepatic bile duct cancer (cholangiocarcinoma), and one adenocarcinoma of the gall bladder. The cell phenotypes, including the histopathology of the primary tumours and in vitro growth characteristics, were determined. We also performed molecular characterisation, including DNA fingerprinting analysis and abnormalities of K-ras, p15, p16, p53, hMLH1, hMSH2, DPC4, beta-catenin, E-cadherin, hOGG1, STK11, and TGF-betaRII genes by PCR-SSCP and sequencing analysis. In addition, we compared the genetic alterations in tumour cell lines and their corresponding tumour tissues. All lines grew as adherent cells. Population doubling times varied from 48-72 h. The culture success rate was 20% (six out of 30 attempts). All cell lines showed (i) relatively high viability; (ii) absence of mycoplasma or bacteria contamination; and (iii) genetic heterogeneity by DNA fingerprinting analysis. Among the lines, three lines had p53 mutations; and homozygous deletions in both p16 and p15 genes were found three and three lines, respectively; one line had a heterozygous missense mutation in hMLH1; E-cadherin gene was hypermethylated in two lines. Since the establishment of biliary tract cancer cell lines has been rarely reported in the literature, these newly established and well characterised biliary tract cancer cell lines would be very useful for studying the biology of biliary tract cancers, particularly those related to hypermethylation of E-cadherin gene in biliary tract cancer. SN - 0007-0920 UR - https://www.unboundmedicine.com/medline/citation/12107841/Establishment_and_characterisation_of_six_human_biliary_tract_cancer_cell_lines_ L2 - http://dx.doi.org/10.1038/sj.bjc.6600440 DB - PRIME DP - Unbound Medicine ER -