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Fed-batch cultivation of baker's yeast followed by nitrogen or carbon starvation: effects on fermentative capacity and content of trehalose and glycogen.
Appl Microbiol Biotechnol. 2002 Jul; 59(2-3):310-7.AM

Abstract

An industrial strain of Saccharomyces cerevisiae (DGI 342) was cultivated in fed-batch cultivations at a specific growth rate of 0.2 h(-1). The yeast was then exposed to carbon or nitrogen starvation for up to 8 h, to study the effect of starvation on fermentative capacity and content of protein, trehalose and glycogen. Nitrogen starvation triggered the accumulation of trehalose and glycogen. After 8 h of starvation, the content of trehalose and glycogen was increased 4-fold and 2-fold, respectively. Carbon starvation resulted in a partial conversion of glycogen into trehalose. The trehalose content increased from 45 to 64 mg (g dry-weight)(-1), whereas the glycogen content in the same period was reduced from 55 to 5 mg (g dry-weight)(-1). Glycogen was consumed faster than trehalose during storage of the starved yeast for 1 month. Nitrogen starvation resulted in a decrease in the protein content of the yeast cells, and the fermentative capacity per gram dry-weight decreased by 40%. The protein content in the carbon-starved yeast increased as a result of starvation due to the fact that the content of glycogen was reduced. The fermentative capacity per gram dry-weight was, however, unaltered.

Authors+Show Affiliations

Centre for Process Biotechnology, BioCentrum-DTU, Technical University of Denmark, Building 223, 2800 Kgs. Lyngby, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12111163

Citation

Jørgensen, H, et al. "Fed-batch Cultivation of Baker's Yeast Followed By Nitrogen or Carbon Starvation: Effects On Fermentative Capacity and Content of Trehalose and Glycogen." Applied Microbiology and Biotechnology, vol. 59, no. 2-3, 2002, pp. 310-7.
Jørgensen H, Olsson L, Rønnow B, et al. Fed-batch cultivation of baker's yeast followed by nitrogen or carbon starvation: effects on fermentative capacity and content of trehalose and glycogen. Appl Microbiol Biotechnol. 2002;59(2-3):310-7.
Jørgensen, H., Olsson, L., Rønnow, B., & Palmqvist, E. A. (2002). Fed-batch cultivation of baker's yeast followed by nitrogen or carbon starvation: effects on fermentative capacity and content of trehalose and glycogen. Applied Microbiology and Biotechnology, 59(2-3), 310-7.
Jørgensen H, et al. Fed-batch Cultivation of Baker's Yeast Followed By Nitrogen or Carbon Starvation: Effects On Fermentative Capacity and Content of Trehalose and Glycogen. Appl Microbiol Biotechnol. 2002;59(2-3):310-7. PubMed PMID: 12111163.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fed-batch cultivation of baker's yeast followed by nitrogen or carbon starvation: effects on fermentative capacity and content of trehalose and glycogen. AU - Jørgensen,H, AU - Olsson,L, AU - Rønnow,B, AU - Palmqvist,E A, Y1 - 2002/05/01/ PY - 2002/01/02/received PY - 2002/04/02/revised PY - 2002/04/02/accepted PY - 2002/7/12/pubmed PY - 2002/9/11/medline PY - 2002/7/12/entrez SP - 310 EP - 7 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 59 IS - 2-3 N2 - An industrial strain of Saccharomyces cerevisiae (DGI 342) was cultivated in fed-batch cultivations at a specific growth rate of 0.2 h(-1). The yeast was then exposed to carbon or nitrogen starvation for up to 8 h, to study the effect of starvation on fermentative capacity and content of protein, trehalose and glycogen. Nitrogen starvation triggered the accumulation of trehalose and glycogen. After 8 h of starvation, the content of trehalose and glycogen was increased 4-fold and 2-fold, respectively. Carbon starvation resulted in a partial conversion of glycogen into trehalose. The trehalose content increased from 45 to 64 mg (g dry-weight)(-1), whereas the glycogen content in the same period was reduced from 55 to 5 mg (g dry-weight)(-1). Glycogen was consumed faster than trehalose during storage of the starved yeast for 1 month. Nitrogen starvation resulted in a decrease in the protein content of the yeast cells, and the fermentative capacity per gram dry-weight decreased by 40%. The protein content in the carbon-starved yeast increased as a result of starvation due to the fact that the content of glycogen was reduced. The fermentative capacity per gram dry-weight was, however, unaltered. SN - 0175-7598 UR - https://www.unboundmedicine.com/medline/citation/12111163/Fed_batch_cultivation_of_baker's_yeast_followed_by_nitrogen_or_carbon_starvation:_effects_on_fermentative_capacity_and_content_of_trehalose_and_glycogen_ L2 - https://dx.doi.org/10.1007/s00253-002-1017-5 DB - PRIME DP - Unbound Medicine ER -