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Induction of micronuclei and erythrocyte alterations in the catfish Clarias batrachus by 2,4-dichlorophenoxyacetic acid and butachlor.
Mutat Res. 2002 Jul 25; 518(2):135-44.MR

Abstract

The micronucleus test (MNT) in fish erythrocytes has increasingly been used to detect the genotoxic effects of environmental mutagens and its frequency is considered to reflect the genotoxic damage to cells, mainly the chromosomes. Besides, morphologically altered erythrocyte is taken as an index of cytotoxicity. Both parameters were used in the present study by two herbicides, 2,4-dichlorophenoxyacetic acid (2,4-D, in 25, 50 and 75ppm concentrations) and 2-chloro-2,6-diethyl-N-(butoxymethyl) acetanilide (butachlor, in 1, 2 and 2.5ppm concentrations) for genotoxic and cytotoxic endpoints. The study was carried out by an in vivo method on peripheral erythrocytes of catfish Clarias batrachus using multiple sampling times (48, 72 and 96h). Cytogenetic preparations were made by haematoxylin-eosin staining technique. Pycnotic and granular micronuclei (MN) were consistently observed irrespective of chemical tested. A wide range of altered cells was also observed. Echinocytes accompanied by altered nuclei and vacuoles were prominent feature of 2,4-D, whereas, anisochromasia and anisocytosis of erythrocytes were characteristic of butachlor. Increase in MN as well as altered cells frequencies were significant. A positive dose-response relationship in all exposures and sampling times was observed. Herbicides used were found to be genotoxic as well as cytotoxic in this fish. The suitability of the adopted parameters for the screening of the aquatic genotoxicants is discussed.

Authors+Show Affiliations

Gene-Tox Laboratory, Department of Zoology, Division of Genetics, Aligarh Muslim University, U.P. 202002, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12113764

Citation

Ateeq, Bushra, et al. "Induction of Micronuclei and Erythrocyte Alterations in the Catfish Clarias Batrachus By 2,4-dichlorophenoxyacetic Acid and Butachlor." Mutation Research, vol. 518, no. 2, 2002, pp. 135-44.
Ateeq B, Abul farah M, Niamat Ali M, et al. Induction of micronuclei and erythrocyte alterations in the catfish Clarias batrachus by 2,4-dichlorophenoxyacetic acid and butachlor. Mutat Res. 2002;518(2):135-44.
Ateeq, B., Abul farah, M., Niamat Ali, M., & Ahmad, W. (2002). Induction of micronuclei and erythrocyte alterations in the catfish Clarias batrachus by 2,4-dichlorophenoxyacetic acid and butachlor. Mutation Research, 518(2), 135-44.
Ateeq B, et al. Induction of Micronuclei and Erythrocyte Alterations in the Catfish Clarias Batrachus By 2,4-dichlorophenoxyacetic Acid and Butachlor. Mutat Res. 2002 Jul 25;518(2):135-44. PubMed PMID: 12113764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of micronuclei and erythrocyte alterations in the catfish Clarias batrachus by 2,4-dichlorophenoxyacetic acid and butachlor. AU - Ateeq,Bushra, AU - Abul farah,M, AU - Niamat Ali,M, AU - Ahmad,Waseem, PY - 2002/7/13/pubmed PY - 2002/8/31/medline PY - 2002/7/13/entrez SP - 135 EP - 44 JF - Mutation research JO - Mutat. Res. VL - 518 IS - 2 N2 - The micronucleus test (MNT) in fish erythrocytes has increasingly been used to detect the genotoxic effects of environmental mutagens and its frequency is considered to reflect the genotoxic damage to cells, mainly the chromosomes. Besides, morphologically altered erythrocyte is taken as an index of cytotoxicity. Both parameters were used in the present study by two herbicides, 2,4-dichlorophenoxyacetic acid (2,4-D, in 25, 50 and 75ppm concentrations) and 2-chloro-2,6-diethyl-N-(butoxymethyl) acetanilide (butachlor, in 1, 2 and 2.5ppm concentrations) for genotoxic and cytotoxic endpoints. The study was carried out by an in vivo method on peripheral erythrocytes of catfish Clarias batrachus using multiple sampling times (48, 72 and 96h). Cytogenetic preparations were made by haematoxylin-eosin staining technique. Pycnotic and granular micronuclei (MN) were consistently observed irrespective of chemical tested. A wide range of altered cells was also observed. Echinocytes accompanied by altered nuclei and vacuoles were prominent feature of 2,4-D, whereas, anisochromasia and anisocytosis of erythrocytes were characteristic of butachlor. Increase in MN as well as altered cells frequencies were significant. A positive dose-response relationship in all exposures and sampling times was observed. Herbicides used were found to be genotoxic as well as cytotoxic in this fish. The suitability of the adopted parameters for the screening of the aquatic genotoxicants is discussed. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/12113764/Induction_of_micronuclei_and_erythrocyte_alterations_in_the_catfish_Clarias_batrachus_by_24_dichlorophenoxyacetic_acid_and_butachlor_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S138357180200075X DB - PRIME DP - Unbound Medicine ER -