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IGF-I, IGF-II, and IGF-receptor-1 transcript and IGF-II protein expression in myostatin knockout mice tissues.
Muscle Nerve. 2002 Jul; 26(1):55-63.MN

Abstract

Semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry were performed to demonstrate whether a correlation exists between insulin-like growth factors (IGFs)-positive regulators of growth-and myostatin, a negative regulator of muscle growth. IGF-I, -II, and IGF-receptor-1 (IGF-R1) mRNA and IGF-II protein expressions were determined in control and myostatin knockout mice tissues. IGF-I gene expressions were similar between control and knockout mice tissues, whereas IGF-II mRNA levels were significantly higher in myostatin knockout mice kidney and soleus muscles than those of control mice (P <.01). IGF-R1 mRNA levels from control mice heart (P <.05) and kidney (P <.01) were significantly higher than in myostatin knockout mice, whereas levels were lower in pectoralis muscle of control mice than knockout mice (P <.01). The strongly IGF-II-positive cells in soleus muscle were more common in myostatin knockout mice and were seen in a few foci in control mice. IGF-II immunoreactivity in both control and myostatin knockout mice kidneys was localized to the epithelium of renal tubules and collecting ducts. Reciprocal changes in the expression of myostatin and IGF-II and IGF-R1 may underlie normal growth of skeletal muscle and other organs in mammals, and the changes in these tissues associated with disease.

Authors+Show Affiliations

Division of Histology and Embryology, Kafkas University, Kars, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

12115949

Citation

Kocamis, Hakan, et al. "IGF-I, IGF-II, and IGF-receptor-1 Transcript and IGF-II Protein Expression in Myostatin Knockout Mice Tissues." Muscle & Nerve, vol. 26, no. 1, 2002, pp. 55-63.
Kocamis H, Gahr SA, Batelli L, et al. IGF-I, IGF-II, and IGF-receptor-1 transcript and IGF-II protein expression in myostatin knockout mice tissues. Muscle Nerve. 2002;26(1):55-63.
Kocamis, H., Gahr, S. A., Batelli, L., Hubbs, A. F., & Killefer, J. (2002). IGF-I, IGF-II, and IGF-receptor-1 transcript and IGF-II protein expression in myostatin knockout mice tissues. Muscle & Nerve, 26(1), 55-63.
Kocamis H, et al. IGF-I, IGF-II, and IGF-receptor-1 Transcript and IGF-II Protein Expression in Myostatin Knockout Mice Tissues. Muscle Nerve. 2002;26(1):55-63. PubMed PMID: 12115949.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IGF-I, IGF-II, and IGF-receptor-1 transcript and IGF-II protein expression in myostatin knockout mice tissues. AU - Kocamis,Hakan, AU - Gahr,Scott A, AU - Batelli,Lori, AU - Hubbs,Ann F, AU - Killefer,John, PY - 2002/7/13/pubmed PY - 2002/8/6/medline PY - 2002/7/13/entrez SP - 55 EP - 63 JF - Muscle & nerve JO - Muscle Nerve VL - 26 IS - 1 N2 - Semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry were performed to demonstrate whether a correlation exists between insulin-like growth factors (IGFs)-positive regulators of growth-and myostatin, a negative regulator of muscle growth. IGF-I, -II, and IGF-receptor-1 (IGF-R1) mRNA and IGF-II protein expressions were determined in control and myostatin knockout mice tissues. IGF-I gene expressions were similar between control and knockout mice tissues, whereas IGF-II mRNA levels were significantly higher in myostatin knockout mice kidney and soleus muscles than those of control mice (P <.01). IGF-R1 mRNA levels from control mice heart (P <.05) and kidney (P <.01) were significantly higher than in myostatin knockout mice, whereas levels were lower in pectoralis muscle of control mice than knockout mice (P <.01). The strongly IGF-II-positive cells in soleus muscle were more common in myostatin knockout mice and were seen in a few foci in control mice. IGF-II immunoreactivity in both control and myostatin knockout mice kidneys was localized to the epithelium of renal tubules and collecting ducts. Reciprocal changes in the expression of myostatin and IGF-II and IGF-R1 may underlie normal growth of skeletal muscle and other organs in mammals, and the changes in these tissues associated with disease. SN - 0148-639X UR - https://www.unboundmedicine.com/medline/citation/12115949/IGF_I_IGF_II_and_IGF_receptor_1_transcript_and_IGF_II_protein_expression_in_myostatin_knockout_mice_tissues_ DB - PRIME DP - Unbound Medicine ER -